EMBO Practical Course on
Solution Scattering from Biological Macromolecules
October 17-24, 2012
EMBL Hamburg, Germany
Organisers: Dmitri Svergun, Al Kikhney, Manfred Roessle, Maxim Petoukhov
The course aims at young biochemists/biophysicists and researchers active in related structural methods with little or no experience in solution scattering. The course will cover basics of SAXS/SANS, instrumentation, data collection, modelling techniques and complementary use with other methods. The students are encouraged to bring their own samples to perform synchrotron solution scattering experiments on-site and the results will be used for practical tutorials and discussed on the last day of the course.
There will be an additional possibility for a combination of SAXS with sample optimization and crystallization experiments, supported by the EU program P-Cube. The selected participants will be offered the opportunity to send their samples in advance to the EMBL Hamburg high-throughput crystallization platform. Depending on the crystallization results, P-Cube may sponsor optimization of the sample conditions, use of the EMBL Sample Preparation and Characterization facility and more extensive SAXS experiments during the course. Please contact Rob Meijers for more information.
 The course is supported by BIOSAXS.
Registration deadline: July 31, 2012
There is no registration fee except the scientists from industry who will be required to pay a fee of €1000. Accommodation and subsistence are covered for accepted applicants. Limited travel grants are available to selected participants coming from labs in countries in need of scientific strengthening. Travel support may also be offered for some of the P-cube sponsored participants.
Tentative programme
Questions and feedback
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Recommended reading
Single copies of the articles below can be downloaded and printed only for the reader's personal research and study.
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Book
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Feigin, L.A. & Svergun, D.I. (1987)
Structure Analysis by Small-Angle X-Ray and Neutron Scattering
© Plenum Press / Springer
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Reviews
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Mertens H.D.T. & Svergun D.I. (2010)
Structural characterization of proteins and complexes using small-angle X-ray solution scattering.
J. Struct. Biol. 172(1), 128-41.
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Svergun, D.I. (2007)
Small-angle scattering studies of macromolecular solutions. J. Appl. Cryst. 40, s10-s17
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Svergun, D.I. & Koch, M.H.J. (2003)
Small-angle scattering studies of biological macromolecules in solution.
Rep. Prog. Phys. 66, 1735-1782
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ATSAS
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Petoukhov, M.V., Franke, D., Shkumatov, A.V., Tria, G., Kikhney, A.G., Gajda, M., Gorba, C., Mertens, H.D.T., Konarev, P.V. and Svergun, D.I. (2012)
New developments in the ATSAS program package for small-angle scattering data analysis.
J. Appl. Cryst. 45, 342-350.
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Petoukhov, M.V., Konarev, P.V., Kikhney A.G. & Svergun, D.I. (2007)
ATSAS 2.1 - towards automated and web-supported small-angle scattering data analysis.
J. Appl. Cryst. 40, s223-s228
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Konarev, P.V., Petoukhov, M.V., Volkov, V.V., & Svergun, D.I. (2006)
ATSAS 2.1, a program package for small-angle scattering data analysis.
J. Appl. Cryst. 39, 277-286.
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PRIMUS
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Konarev, P.V., Volkov, V.V., Sokolova, A.V., Koch M.H.J. & Svergun, D.I. (2003)
PRIMUS: a Windows PC-based system for small-angle scattering data analysis.
J. Appl. Cryst. 36, 1277-1282.
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GNOM
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Svergun D.I. (1992)
Determination of the regularization parameter in indirect-transform methods using perceptual criteria.
J. Appl. Crystallogr. 25, 495-503
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DAMMIN
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Svergun, D. I. (1999)
Restoring low resolution structure of biological macromolecules from solution scattering using simulated annealing.
Biophys. J. 76, 2879-2886.
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DAMMIF
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Franke, D. and Svergun, D.I. (2009)
DAMMIF, a program for rapid ab-initio shape determination in small-angle scattering. J. Appl. Cryst., 42, 342-346.
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GASBOR
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Svergun, D.I., Petoukhov, M.V. & Koch, M.H.J. (2001)
Determination of domain structure of proteins from X-ray solution scattering.
Biophys. J. 80, 2946-2953.
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CRYSOL
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Svergun D.I., Barberato C., & Koch M.H.J. (1995)
CRYSOL - a program to evalate X-ray solution scattering of biological macromolecules from atomic coordinates.
J. Appl. Crystallogr. 28, 768-773
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MASSHA
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Konarev, P.V., Petoukhov, M.V. & Svergun, D.I. (2001)
MASSHA - a graphic system for rigid body modelling of macromolecular complexes against solution scattering data.
J. Appl. Crystallogr. 34, 527-532.
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SASREF,
BUNCH,
CORAL
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Petoukhov, M.V. & Svergun, D.I. (2005)
Global rigid body modelling of macromolecular complexes against
small-angle scattering data.
Biophys. J. 89, 1237-1250
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EOM
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Bernado, P., Mylonas, E., Petoukhov, M.V., Blackledge, M., Svergun, D.I. (2007)
Structural Characterization of Flexible Proteins Using Small-Angle X-ray Scattering. J. Am. Chem. Soc. 129(17), 5656-5664
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