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EMBL Hamburg Biological
Small Angle Scattering
BioSAXS
  Home  >  Courses  >  EMBO' 2012

Solution Scattering from Biological Macromolecules October 17-24, 2012
EMBL Hamburg, Germany
Organisers: Dmitri Svergun, Al Kikhney, Maxim Petoukhov, Manfred Roessle

The course aims at young biochemists/biophysicists and researchers active in related structural methods with little or no experience in solution scattering. The course will cover basics of SAXS/SANS, instrumentation, data collection, modelling techniques and complementary use with other methods. The students are encouraged to bring their own samples to perform synchrotron solution scattering experiments on-site and the results will be used for practical tutorials and discussed on the last day of the course.

P3 There will be an additional possibility for a combination of SAXS with sample optimization and crystallization experiments, supported by the EU program P-Cube. The selected participants will be offered the opportunity to send their samples in advance to the EMBL Hamburg high-throughput crystallization platform. Depending on the crystallization results, P-Cube may sponsor optimization of the sample conditions, use of the EMBL Sample Preparation and Characterization facility and more extensive SAXS experiments during the course. Please contact Rob Meijers for more information.
BI((O))SAXS   The course is supported by BIOSAXS.

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Abstracts of the talks and slides in PDF

Abstract X-ray scattering – the basics (3MB) – Manfred Roessle
Abstract Neutron scattering – the basics (8MB) – Jill Trewhella
Abstract Validation of biomacromolecular structures (17MB) – Gerard Kleywegt
Abstract Sample preparation and characterisation (3MB) – Rob Meijers
Abstract Data reduction and processing (7MB) – Al Kikhney
  Data reduction and processing tutorial (3MB) – Petr Konarev
Abstract Ab initio methods: how they work (10MB) – Dmitri Svergun
  Ab initio modelling: DAMMIN and DAMMIF (1MB) – Daniel Franke
  Ab initio modelling: GASBOR and MONSA (1MB) – Maxim Petoukhov
Abstract Rigid body refinement (basics) (6MB) – Dmitri Svergun
Abstract Form and structure factors, interactions, modelling (4MB) – Jan Skov Pedersen
  EOM tutorial (4MB) – Giancarlo Tria
Abstract Joint use of SAXS and NMR (10MB) – Annalisa Pastore
Abstract Joint use of SAS and AUC (7MB) – Olwyn Byron
Abstract Maximum occurrence of conformations from average data (28MB) – Claudio Luchinat
Abstract Ubiquitin conjugation and deconjugation in DNA regulation (6MB) – Titia Sixma
Abstract Structural studies of lipid systems (3MB) – Regine Willumeit
Abstract High brilliance synchrotrons for SAXS and WAXS (9MB) – Robert Fischetti
Abstract Combining microfluidics and synchrotron SAXS (5MB) – Lise Arleth

Recommended reading

Single copies of the articles below can be downloaded and printed only for the reader's personal research and study.
Book Feigin, L.A. & Svergun, D.I. (1987)
Structure Analysis by Small-Angle X-Ray and Neutron Scattering © Plenum Press / Springer
Reviews Mertens H.D.T. & Svergun D.I. (2010)
Structural characterization of proteins and complexes using small-angle X-ray solution scattering. J. Struct. Biol. 172(1), 128-41
  Svergun, D.I. (2007)
Small-angle scattering studies of macromolecular solutions. J. Appl. Cryst. 40, s10-s17
  Svergun, D.I. & Koch, M.H.J. (2003)
Small-angle scattering studies of biological macromolecules in solution.
Rep. Prog. Phys. 66, 1735-1782
ATSAS Petoukhov, M.V., Franke, D., Shkumatov, A.V., Tria, G., Kikhney, A.G., Gajda, M., Gorba, C., Mertens, H.D.T., Konarev, P.V. and Svergun, D.I. (2012)
New developments in the ATSAS program package for small-angle scattering data analysis.
J. Appl. Cryst. 45, 342-350
  Petoukhov, M.V., Konarev, P.V., Kikhney A.G. & Svergun, D.I. (2007)
ATSAS 2.1 - towards automated and web-supported small-angle scattering data analysis.
J. Appl. Cryst. 40, s223-s228
  Konarev, P.V., Petoukhov, M.V., Volkov, V.V., & Svergun, D.I. (2006)
ATSAS 2.1, a program package for small-angle scattering data analysis.
J. Appl. Cryst. 39, 277-286
PRIMUS Konarev, P.V., Volkov, V.V., Sokolova, A.V., Koch M.H.J. & Svergun, D.I. (2003)
PRIMUS: a Windows PC-based system for small-angle scattering data analysis. J. Appl. Cryst. 36, 1277-1282
GNOM Svergun D.I. (1992)
Determination of the regularization parameter in indirect-transform methods using perceptual criteria. J. Appl. Crystallogr. 25, 495-503
DAMMIN Svergun, D. I. (1999)
Restoring low resolution structure of biological macromolecules from solution scattering using simulated annealing. Biophys. J. 76, 2879-2886
DAMMIF Franke, D. and Svergun, D.I. (2009)
DAMMIF, a program for rapid ab-initio shape determination in small-angle scattering.
J. Appl. Cryst., 42, 342-346
GASBOR Svergun, D.I., Petoukhov, M.V. & Koch, M.H.J. (2001)
Determination of domain structure of proteins from X-ray solution scattering. Biophys. J. 80, 2946-2953
CRYSOL Svergun D.I., Barberato C., & Koch M.H.J. (1995)
CRYSOL - a program to evalate X-ray solution scattering of biological macromolecules from atomic coordinates. J. Appl. Crystallogr. 28, 768-773
SASREF,
BUNCH
Petoukhov, M.V. & Svergun, D.I. (2005)
Global rigid body modelling of macromolecular complexes against small-angle scattering data. Biophys. J. 89, 1237-1250
MASSHA Konarev, P.V., Petoukhov, M.V. & Svergun, D.I. (2001)
MASSHA - a graphic system for rigid body modelling of macromolecular complexes against solution scattering data. J. Appl. Crystallogr. 34, 527-532
EOM Bernado, P., Mylonas, E., Petoukhov, M.V., Blackledge, M., Svergun, D.I. (2007)
Structural Characterization of Flexible Proteins Using Small-Angle X-ray Scattering. J. Am. Chem. Soc. 129(17), 5656-5664

  Last modified: October 25, 2012

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