Small Angle X-Ray Scattering
Beamline X33 is a bending magnet beamline for biological small-angle scattering on the DORIS Fan D, located in the HASYLAB Hall 3. It uses a fixed wavelength of 1.5 A. The endstation is equipped with a photon counting Pilatus 1M-W pixel detector (67 x 420 mm2) and an additional Pilatus 300K-W pixel detector (33.5 x 254 mm2). The beamline is optimised for low background data collection from macromolecular solutions. A two-detector setup is used to simultaneously collect small and wide angle scattering (SAXS/WAXS) patterns.
Contacts
Scientists in charge
Dmitri Svergun
| Tel: | +49 40 89902-125 |
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| E-mail: | svergun@embl-hamburg.de |
Manfred Roessle
| Tel: | +49 40 89902-192 |
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| E-mail: | manfred.roessle@embl-hamburg.de |
Small angle X-ray scattering beamline X33
| Tel: | +49 40 89902-153 |
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Experimental possibilities
- SAXS/WAXS experiments on macromolecules ranging from a few kDa to GDa
- Time resolved measurements and temperature scans
- Automated liquid handling sample changer
- Sample holders for solutions, gels, films, powders
- Resolution range: 700 to 10 A (SAXS); 17 to 4 A (WAXS)
- Typical exposure times: 15-120 s
Facts
| Synchrotron source | DORIS bending magnet | |
| Monochromator | Asymmetric cut 7° crystal Si (111), horizontally focusing | |
| Mirror | Rh coated, Zerodur, vertically focusing | |
| Acquisition system | CANbus, TINE | |
| Wavelength | 1.5 A | |
| Focal spot | 2 mm x 0.6 mm | |
| Flux | 5 x 1011 ph/s/100mA | |
| Slits | 4 pairs, tungsten | |
| Small automated sample changer | 8 samples (200 ?l) 3 buffers (1.5 ml) temperature controlled storage (5-45°C) |
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| Large automated sample changer | 2 x 96 samples (200 ?l) 2 x 24 buffers (1.5 ml) temperature controlled storage (10-40°C) |
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| Vacuum cell | 30 ?l, polycarbonate windows, automated filling and rinsing, temperature controlled (5-50°C) | |
| In-air cell | 100 ?l, mica windows, manual filling and rinsing – for viscous and solid samples temperature controlled (0-80°C) |
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| Detectors | Photon counting Pilatus 1M-W pixel detector (67 x 420 mm2); Photon counting Pilatus 300K-W pixel detector (33.5 x 254 mm2); Mar345 image plate (345 mm2) |
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| Sample-detector distance | Standard: 2.7 m (SAXS), 0.75 m (WAXS) |
Recent changes, upgrades and developments
Roessle, M.W., Klaering, R., Ristau, U., Robrahn, B. Jahn, D., Gehrmann, T., Konarev, P. V., Round, A., Fiedler, S., Hermes, S. & Svergun, D. I. (2007). Upgrade of the Small Angle X-ray scattering Beamline X33 at the European Molecular Biology Laboratory, Hamburg. J. Appl. Crystallogr., 40, s190-s194.
Round, A.R., Franke, D., Moritz, S., Huchler, R., Fritsche, M., Malthan, D., Klaering, R., Svergun, D.I. & Roessle, M. (2008) Automated sample-changing robot for solution scattering experiments at the EMBL Hamburg SAXS station X33. J. Appl. Crystallogr., 41, 913-917.