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Staff at EMBL

Dr. Matthias Wilmanns

Head of Outstation and Senior Scientist
Wilmanns Group

EMBL Hamburg c/o DESY, Notkestraße 85, 22603 Hamburg, Germany

E-mail: send a mail


PhD 1990, University of Basel.
Postdoctoral research at the University of California, Los Angeles.
Group leader at EMBL Heidelberg 1993-1997.
Head of EMBL Hamburg since 1997.
Research Director of the Center for Structural Systems Biology (CSSB) since 2014.


Induction of insulin-like growth factor 1 splice forms by subfragments of myofibrillar proteins. Kravchenko, I.V., Furalyov, V.A., Chatziefthimiou, S., Wilmanns, M. & Popov, V.O. Mol Cell Endocrinol. 2014 Aug 21. pii: S0303-7207(14)00247-0. doi:10.1016/j.mce.2014.08.010. Europe PMC

Active site conformational changes upon reaction intermediate biotinyl-5'-AMP binding in biotin protein ligase from Mycobacterium tuberculosis. Ma, Q., Akhter, Y., Wilmanns, M. & Ehebauer, M.T. Protein Sci. 2014 Apr 9. doi: 10.1002/pro.2475. Europe PMC

Development of Accessible Peptidic Tool Compounds To Study the Phosphatase PTP1B in Intact Cells. Meyer, C., Hoeger, B., Temmerman, K., Tatarek-Nossol, M., Pogenberg, V., Bernhagen, J., Wilmanns, M., Kapurniotu, A. & Köhn, M. ACS Chem Biol. 2014 Mar 21;9(3):769-76. doi: 10.1021/cb400903u. Epub 2014 Jan 15. Europe PMC

Role of kappa-->lambda light-chain constant-domain switch in the structure and functionality of A17 reactibody. Ponomarenko, N., Chatziefthimiou, S.D., Kurkova, I., Mokrushina, Y., Mokrushina, Y., Stepanova, A., Smirnov, I., Avakyan, M., Bobik, T., Mamedov, A., Mitkevich, V., Belogurov A, J.r, Fedorova, O.S., Dubina, M., Golovin, A., Lamzin, V., Friboulet, A., Makarov, A.A., Wilmanns, M. & Gabibov, A. Acta Crystallogr D Biol Crystallogr. 2014 Mar;70(Pt 3):708-19. doi:10.1107/S1399004713032446. Epub 2014 Feb 15. Europe PMC

Design of a bZip Transcription Factor with Homo/Heterodimer-Induced DNA-Binding Preference. Pogenberg, V., Consani Textor, L., Vanhille, L., Holton, S.J., Sieweke, M.H. & Wilmanns, M. Structure. 2014 Feb 11. pii: S0969-2126(14)00009-4. doi:10.1016/j.str.2013.12.017. Europe PMC

A PEF/Y Substrate Recognition and Signature Motif Plays a Critical Role in DAPK-Related Kinase Activity. Temmerman, K., de Diego, I., Pogenberg, V., Simon, B., Jonko, W., Li, X. & Wilmanns, M. Chem Biol. 2014 Jan 14. pii: S1074-5521(13)00457-2. doi:10.1016/j.chembiol.2013.12.008. Europe PMC

The 11th International Conference on Biology and Synchrotron Radiation. Wilmanns, M. & Svergun, D.I. J Synchrotron Radiat. 2014 Jan;21(Pt 1):280-2. doi: 10.1107/S1600577513030609.Epub 2013 Dec 3. Europe PMC

WXG100 protein superfamily consists of three subfamilies and exhibits an alpha-helical C-terminal conserved residue pattern. Poulsen, C., Panjikar, S., Holton, S.J., Wilmanns, M. & Song, Y.H. PLoS One. 2014 Feb 26;9(2):e89313. doi: 10.1371/journal.pone.0089313. eCollection2014. Europe PMC

Catalytic mechanism of alpha-phosphate attack in dUTPase is revealed by X-ray crystallographic snapshots of distinct intermediates, 31P-NMR spectroscopy and reaction path modelling. Barabas, O., Nemeth, V., Bodor, A., Perczel, A., Rosta, E., Kele, Z., Zagyva, I., Szabadka, Z., Grolmusz, V.I., Wilmanns, M. & Vertessy, B.G. Nucleic Acids Res. 2013 Dec;41(22):10542-55. doi: 10.1093/nar/gkt756. Epub 2013Aug 27. Europe PMC

Structural and functional diversity in the activity and regulation of DAPK-related protein kinases. Temmerman, K., Simon, B. & Wilmanns, M. FEBS J. 2013 Nov;280(21):5533-50. doi: 10.1111/febs.12384. Epub 2013 Jul 5. Europe PMC

Identification of Xin-repeat proteins as novel ligands of the SH3 domains of nebulin and nebulette and analysis of their interaction during myofibril formation and remodeling. Eulitz, S., Sauer, F., Pelissier, M.C., Boisguerin, P., Molt, S., Schuld, J., Orfanos, Z., Kley, R.A., Volkmer, R., Wilmanns, M., Kirfel, G., van der Ven, P.F. & Furst, D.O. Mol Biol Cell. 2013 Oct;24(20):3215-26. doi: 10.1091/mbc.E13-04-0202. Epub 2013Aug 28. Europe PMC

Structural and biochemical characterisation of a NAD(+)-dependent alcohol dehydrogenase from Oenococcus oeni as a new model molecule for industrial biotechnology applications. Elleuche, S., Fodor, K., Klippel, B., von der Heyde, A., Wilmanns, M. & Antranikian, G. Appl Microbiol Biotechnol. 2013 Oct;97(20):8963-75. doi:10.1007/s00253-013-4725-0. Epub 2013 Feb 6. Europe PMC

Structural and biochemical characterization of Rv2140c, a phosphatidylethanolamine-binding protein from Mycobacterium tuberculosis. Eulenburg, G., Higman, V.A., Diehl, A., Wilmanns, M. & Holton, S.J. FEBS Lett. 2013 Sep 17;587(18):2936-42. doi: 10.1016/j.febslet.2013.07.038. Epub2013 Jul 29. Europe PMC

P450 BM3 crystal structures reveal the role of the charged surface residue Lys/Arg184 in inversion of enantioselective styrene epoxidation. Shehzad, A., Panneerselvam, S., Linow, M., Bocola, M., Roccatano, D., Mueller-Dieckmann, J., Wilmanns, M. & Schwaneberg, U. Chem Commun (Camb). 2013 May 21;49(41):4694-6. doi: 10.1039/c3cc39076d. Epub 2013Apr 15. Europe PMC

Elucidating human phosphatase-substrate networks. Li, X., Wilmanns, M., Thornton, J. & Köhn, M. Sci Signal. 2013 May 14;6(275):rs10. doi: 10.1126/scisignal.2003203. Europe PMC

A unique Oct4 interface is crucial for reprogramming to pluripotency. Esch, D., Vahokoski, J., Groves, M.R., Pogenberg, V., Cojocaru, V., Vom Bruch, H., Han, D., Drexler, H.C., Arauzo-Bravo, M.J., Ng, C.K., Jauch, R., Wilmanns, M. & Scholer, H.R. Nat Cell Biol. 2013 Feb 3;15(3):295-301. doi: 10.1038/ncb2680. Epub 2013 Feb 3. Europe PMC

Crystal structure of the S187F variant of human liver alanine: aminotransferase associated with primary hyperoxaluria type I and its functional implications. Oppici, E., Fodor, K., Paiardini, A., Williams, C., Voltattorni, C.B., Wilmanns, M. & Cellini, B. Proteins. 2013 Aug;81(8):1457-65. doi: 10.1002/prot.24300. Epub 2013 Jun 1. Europe PMC

A disulphide bond in the E2 enzyme Pex4p modulates ubiquitin-conjugating activity. Williams, C., van den Berg, M., Stanley, W.A., Wilmanns, M. & Distel, B. Sci Rep. 2013;3:2212. doi: 10.1038/srep02212. Europe PMC

Catalysis uncoupling in a glutamine amidotransferase bienzyme by unblocking the glutaminase active site. List, F., Vega, M.C., Razeto, A., Hager, M.C., Sterner, R. & Wilmanns, M. Chem Biol. 2012 Dec 21;19(12):1589-99. doi: 10.1016/j.chembiol.2012.10.012. Europe PMC

Restricted leucine zipper dimerization and specificity of DNA recognition of the melanocyte master regulator MITF. Pogenberg, V., Ogmundsdottir, M.H., Bergsteinsdottir, K., Schepsky, A., Phung, B., Deineko, V., Milewski, M., Steingrimsson, E. & Wilmanns, M. Genes Dev. 2012 Dec 1;26(23):2647-58. doi: 10.1101/gad.198192.112. Europe PMC

Structural characterization of a D-isomer specific 2-hydroxyacid dehydrogenase from Lactobacillus delbrueckii ssp. bulgaricus. Holton, S.J., Anandhakrishnan, M., Geerlof, A. & Wilmanns, M. J Struct Biol. 2012 Oct 27. pii: S1047-8477(12)00291-2. doi:10.1016/j.jsb.2012.10.009. Europe PMC

Mycobacterium tuberculosis prokaryotic ubiquitin-like protein-deconjugating enzyme is an unusual aspartate amidase. Burns, K.E., McAllister, F.E., Schwerdtfeger, C., Mintseris, J., Cerda-Maira, F., Noens, E.E., Wilmanns, M., Hubbard, S.R., Melandri, F., Ovaa, H., Gygi, S.P. & Darwin, K.H. J Biol Chem. 2012 Oct 26;287(44):37522-9. doi: 10.1074/jbc.M112.384784. Epub 2012Aug 31. Europe PMC

The role of active-site Phe87 in modulating the organic co-solvent tolerance of cytochrome P450 BM3 monooxygenase. Kuper, J., Tee, K.L., Wilmanns, M., Roccatano, D., Schwaneberg, U. & Wong, T.S. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Sep 1;68(Pt 9):1013-7.doi: 10.1107/S1744309112031570. Epub 2012 Aug 29. Europe PMC

Monospecific inhibitors show that both mannan-binding lectin-associated serine protease-1 (MASP-1) and -2 Are essential for lectin pathway activation and reveal structural plasticity of MASP-2. Heja, D., Harmat, V., Fodor, K., Wilmanns, M., Dobo, J., Kekesi, K.A., Zavodszky, P., Gal, P. & Pal, G. J Biol Chem. 2012 Jun 8;287(24):20290-300. doi: 10.1074/jbc.M112.354332. Epub2012 Apr 16. Europe PMC

Molecular requirements for peroxisomal targeting of alanine-glyoxylate aminotransferase as an essential determinant in primary hyperoxaluria type 1. Fodor, K., Wolf, J., Erdmann, R., Schliebs, W. & Wilmanns, M. PLoS Biol. 2012;10(4):e1001309. doi: 10.1371/journal.pbio.1001309. Epub 2012 Apr17. Europe PMC

Superhelical architecture of the myosin filament-linking protein myomesin with unusual elastic properties. Pinotsis, N., Chatziefthimiou, S.D., Berkemeier, F., Beuron, F., Mavridis, I.M., Konarev, P.V., Svergun, D.I., Morris, E., Rief, M. & Wilmanns, M. PLoS Biol. 2012 Feb;10(2):e1001261. doi: 10.1371/journal.pbio.1001261. Epub 2012Feb 14. Europe PMC

Two immunoglobulin tandem proteins with a linking beta-strand reveal unexpected differences in cooperativity and folding pathways. Steward, A., Chen, Q., Chapman, R.I., Borgia, M.B., Rogers, J.M., Wojtala, A., Wilmanns, M. & Clarke, J. J Mol Biol. 2012 Feb 10;416(1):137-47. doi: 10.1016/j.jmb.2011.12.012. Epub 2011Dec 13. Europe PMC

Insights into ubiquitin-conjugating enzyme/ co-activator interactions from the structure of the Pex4p:Pex22p complex. Williams, C., van den Berg, M., Panjikar, S., Stanley, W.A., Distel, B. & Wilmanns, M. EMBO J. 2012 Jan 18;31(2):391-402. doi: 10.1038/emboj.2011.411. Epub 2011 Nov 15. Europe PMC

Telethonin deficiency is associated with maladaptation to biomechanical stress in the mammalian heart. Knoll, R., Linke, W.A., Zou, P., Miocic, S., Kostin, S., Buyandelger, B., Ku, C.H., Neef, S., Bug, M., Schafer, K., Knoll, G., Felkin, L.E., Wessels, J., Toischer, K., Hagn, F., Kessler, H., Didie, M., Quentin, T., Maier, L.S., Teucher, N., Unsold, B., Schmidt, A., Birks, E.J., Gunkel, S., Lang, P., Granzier, H., Zimmermann, W.H., Field, L.J., Faulkner, G., Dobbelstein, M., Barton, P.J., Sattler, M., Wilmanns, M. & Chien, K.R. Circ Res. 2011 Sep 16;109(7):758-69. Epub 2011 Jul 28. Europe PMC

Fast-folding alpha-helices as reversible strain absorbers in the muscle protein myomesin. Berkemeier, F., Bertz, M., Xiao, S., Pinotsis, N., Wilmanns, M., Grater, F. & Rief, M. Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14139-44. Epub 2011 Aug 8. Europe PMC

The progress made in determining the Mycobacterium tuberculosis structural proteome. Ehebauer, M.T. & Wilmanns, M. Proteomics. 2011 Aug;11(15):3128-33. doi: 10.1002/pmic.201000787. Epub 2011 Jun14. Europe PMC

Structural insights into transcription complexes. Berger, I., Blanco, A.G., Boelens, R., Cavarelli, J., Coll, M., Folkers, G.E., Nie, Y., Pogenberg, V., Schultz, P., Wilmanns, M., Moras, D. & Poterszman, A. J Struct Biol. 2011 Aug;175(2):135-46. doi: 10.1016/j.jsb.2011.04.015. Epub 2011May 6. Europe PMC

Structural features of peroxisomal catalase from the yeast Hansenula polymorpha. Pena-Soler, E., Vega, M.C., Wilmanns, M. & Williams, C. Acta Crystallogr D Biol Crystallogr. 2011 Aug;67(Pt 8):690-8. Epub 2011Jul 12. Europe PMC

Expression of protein complexes using multiple Escherichia coli protein co-expression systems: A benchmarking study. Busso, D., Peleg, Y., Heidebrecht, T., Romier, C., Jacobovitch, Y., Dantes, A., Salim, L., Troesch, E., Schuetz, A., Heinemann, U., Folkers, G.E., Geerlof, A., Wilmanns, M., Polewacz, A., Quedenau, C., Bussow, K., Adamson, R., Blagova, E., Walton, J., Cartwright, J.L., Bird, L.E., Owens, R.J., Berrow, N.S., Wilson, K.S., Sussman, J.L., Perrakis, A. & Celie, P.H. J Struct Biol. 2011 Aug;175(2):159-70. Epub 2011 Mar 5. Europe PMC

Rapid development of genetically encoded FRET reporters. Piljic, A., de Diego, I., Wilmanns, M. & Schultz, C. ACS Chem Biol. 2011 Jul 15;6(7):685-91. Epub 2011 Apr 28. Europe PMC

Related (betaalpha)8-barrel proteins in histidine and tryptophan biosynthesis: a paradigm to study enzyme evolution. List, F., Sterner, R. & Wilmanns, M. Chembiochem. 2011 Jul 4;12(10):1487-94. doi: 10.1002/cbic.201100082. Epub2011 Jun 8. Europe PMC

Bisubstrate specificity in histidine/tryptophan biosynthesis isomerase from Mycobacterium tuberculosis by active site metamorphosis. Due, A.V., Kuper, J., Geerlof, A., von Kries, J.P. & Wilmanns, M. Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3554-9. Epub 2011 Feb 14. Europe PMC

Improved mycobacterial protein production using a Mycobacterium smegmatis groEL1deltaC expression strain. Noens, E.E., Williams, C., Anandhakrishnan, M., Poulsen, C., Ehebauer, M.T. & Wilmanns, M. BMC Biotechnol. 2011 Mar 25;11(1):27. Europe PMC

The Peroxisomal Targeting Signal 1 in sterol carrier protein 2 is autonomous and essential for receptor recognition. Williams, C.P., Schueller, N., Thompson, C.A., van den Berg, M., Van Haren, S.D., Erdmann, R., Bond, C.S., Distel, B., Schliebs, W., Wilmanns, M. & Stanley, W.A. BMC Biochem. 2011 Mar 4;12:12. doi: 10.1186/1471-2091-12-12. Europe PMC

Structural synthetic biotechnology: from molecular structure to predictable design for industrial strain development. Chen, Z., Wilmanns, M. & Zeng, A.P. Trends Biotechnol. 2010 Oct;28(10):534-42. Epub 2010 Aug 19. Europe PMC

The peroxisomal receptor Pex19p forms a helical mPTS recognition domain. Schueller, N., Holton, S.J., Fodor, K., Milewski, M., Konarev, P., Stanley, W.A., Wolf, J., Erdmann, R., Schliebs, W., Song, Y.H. & Wilmanns, M. EMBO J. 2010 Aug 4;29(15):2491-500. Epub 2010 Jun 8. Europe PMC

Proteome-wide identification of mycobacterial pupylation targets. Poulsen, C., Akhter, Y., Jeon, A.H., Schmitt-Ulms, G., Meyer, H.E., Stefanski, A., Stuhler, K., Wilmanns, M. & Song, Y.H. Mol Syst Biol. 2010 Jul 13;6:386. Europe PMC

Molecular basis of the head-to-tail assembly of giant muscle proteins obscurin-like 1 and titin. Sauer, F., Vahokoski, J., Song, Y.H. & Wilmanns, M. EMBO Rep. 2010 Jul;11(7):534-40. Epub 2010 May 21. Europe PMC

Highlight: of systems and structures. Heinz, D.W., Betzel, C. & Wilmanns, M. Biol Chem. 2010 Jul;391(7):717-8. Europe PMC

Stoichiometric protein complex formation and over-expression using the prokaryotic native operon structure. Poulsen, C., Holton, S., Geerlof, A., Wilmanns, M. & Song, Y.H. FEBS Lett. 2010 Feb 19;584(4):669-74. Epub 2010 Jan 18. Europe PMC

Molecular basis of the death-associated protein kinase-calcium/calmodulin regulator complex. de Diego, I., Kuper, J., Bakalova, N., Kursula, P. & Wilmanns, M. Sci Signal. 2010 Jan 26;3(106):ra6. Europe PMC

Solution structure of human Pex5.Pex14.PTS1 protein complexes obtained by small angle X-ray scattering. Shiozawa, K., Konarev, P.V., Neufeld, C., Wilmanns, M. & Svergun, D.I. J Biol Chem. 2009 Sep 11;284(37):25334-42. Epub 2009 Jul 6. Europe PMC

The titin-telethonin complex is a directed, superstable molecular bond in the muscle Z-disk. Bertz, M., Wilmanns, M. & Rief, M. Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13307-133310. Epub 2009 Jul21. Europe PMC

Structure-based prediction of the Saccharomyces cerevisiae SH3-ligand interactions. Fernandez-Ballester, G., Beltrao, P., Gonzalez, J.M., Song, Y.H., Wilmanns, M., Valencia, A. & Serrano, L. J Mol Biol. 2009 May 15;388(4):902-16. Epub 2009 Mar 24. Europe PMC

Accurate solution structures of proteins from X-ray data and a minimal set of NMR data: calmodulin-peptide complexes as examples. Bertini, I., Kursula, P., Luchinat, C., Parigi, G., Vahokoski, J., Wilmanns, M. & Yuan, J. J Am Chem Soc. 2009 Apr 15;131(14):5134-44. Europe PMC

Structural basis for competitive interactions of Pex14 with the import receptors Pex5 and Pex19. Neufeld, C., Filipp, F.V., Simon, B., Neuhaus, A., Schuller, N., David, C., Kooshapur, H., Madl, T., Erdmann, R., Schliebs, W., Wilmanns, M. & Sattler, M. EMBO J. 2009 Mar 18;28(6):745-54. Epub 2009 Feb 5. Europe PMC

Mechanical network in titin immunoglobulin from force distribution analysis. Stacklies, W., Vega, M.C., Wilmanns, M. & Grater, F. PLoS Comput Biol. 2009 Mar;5(3):e1000306. Epub 2009 Mar 13. Europe PMC

Crystal structure of the resuscitation-promoting factor (DeltaDUF)RpfB from M. tuberculosis. Ruggiero, A., Tizzano, B., Pedone, E., Pedone, C., Wilmanns, M. & Berisio, R. J Mol Biol. 2009 Jan 9;385(1):153-62. Epub 2008 Oct 22. Europe PMC

Terminal assembly of sarcomeric filaments by intermolecular beta-sheet formation. Pinotsis, N., Abrusci, P., Djinovic-Carugo, K. & Wilmanns, M. Trends Biochem Sci. 2009 Jan;34(1):33-9. Epub 2008 Nov 6. Europe PMC

Protein assemblies with palindromic structure motifs. Pinotsis, N. & Wilmanns, M. Cell Mol Life Sci. 2008 Oct;65(19):2953-6. Europe PMC

Regulation of the transcription factor Ets-1 by DNA-mediated homo-dimerization. Lamber, E.P., Vanhille, L., Textor, L.C., Kachalova, G.S., Sieweke, M.H. & Wilmanns, M. EMBO J. 2008 Jul 23;27(14):2006-17. Epub 2008 Jun 19. Europe PMC

Bimolecular fluorescence complementation in structural biology. Song, Y.H. & Wilmanns, M. Methods. 2008 Jul;45(3):219-22. Epub 2008 Jul 9. Europe PMC

Probing lipid- and drug-binding domains with fluorescent dyes. Black, S.L., Stanley, W.A., Filipp, F.V., Bhairo, M., Verma, A., Wichmann, O., Sattler, M., Wilmanns, M. & Schultz, C. Bioorg Med Chem. 2008 Feb 1;16(3):1162-73. Epub 2007 Nov 19. Europe PMC

Molecular basis of the C-terminal tail-to-tail assembly of the sarcomeric filament protein myomesin. Pinotsis, N., Lange, S., Perriard, J.C., Svergun, D.I. & Wilmanns, M. EMBO J. 2008 Jan 9;27(1):253-64. Epub 2007 Dec 6. Europe PMC

Low resolution structural models of the basic helix-loop-helix leucine zipper domain of upstream stimulatory factor 1 and its complexes with DNA from small angle X-ray scattering data. Lamber, E.P., Wilmanns, M. & Svergun, D.I. Biophys J. 2008 Jan 1;94(1):193-7. Epub 2007 Sep 7. Europe PMC

High-resolution Structural Analysis of Mammalian Profilin 2a Complex Formation with Two Physiological Ligands: The Formin Homology 1 Domain of mDia1 and the Proline-rich Domain of VASP. Kursula, P., Kursula, I., Massimi, M., Song, Y.H., Downer, J., Stanley, W.A., Witke, W. & Wilmanns, M. J Mol Biol. 2007 Oct 24;. Europe PMC

Protein translocation into peroxisomes by ring-shaped import receptors. Stanley, W.A., Fodor, K., Marti-Renom, M.A., Schliebs, W. & Wilmanns, M. FEBS Lett. 2007 Oct 16;581(25):4795-802. Epub 2007 Sep 11. Europe PMC

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of a resuscitation-promoting factor from Mycobacterium tuberculosis. Ruggiero, A., Tizzano, B., Geerlof, A., Pedone, E., Pedone, C., Wilmanns, M. & Berisio, R. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Oct 1;63(Pt10):870-3. Epub 2007 Sep 19. Europe PMC

Second SH3 Domain of Ponsin Solved from Powder Diffraction. Margiolaki, I., Wright, J.P., Wilmanns, M., Fitch, A.N. & Pinotsis, N. J Am Chem Soc. 2007 Sep 5;. Europe PMC

Rigid conformation of an immunoglobulin domain tandem repeat in the A-band of the elastic muscle protein titin. Muller, S., Lange, S., Gautel, M. & Wilmanns, M. J Mol Biol. 2007 Aug 10;371(2):469-80. Epub 2007 May 25. Europe PMC

Expression, purification, crystallization and preliminary crystallographic analysis of the mouse transcription factor MafB in complex with its DNA-recognition motif Cmare. Textor, L.C., Wilmanns, M. & Holton, S.J. Acta Crystallograph Sect F Struct Biol Cryst Commun. 2007 Aug 1;63(Pt8):657-61. Epub 2007 Jul 7. Europe PMC

Structure-based approaches to drug discovery against tuberculosis. Holton, S.J., Weiss, M.S., Tucker, P.A. & Wilmanns, M. Curr Protein Pept Sci. 2007 Aug;8(4):365-75. Europe PMC

Paxillin and ponsin interact in nascent costameres of muscle cells. Gehmlich, K., Pinotsis, N., Hayess, K., van der Ven, P.F., Milting, H., El Banayosy, A., Korfer, R., Wilmanns, M., Ehler, E. & Furst, D.O. J Mol Biol. 2007 Jun 8;369(3):665-82. Epub 2007 Mar 24. Europe PMC

Understanding a mechanism of organic cosolvent inactivation in heme monooxygenase P450 BM-3. Kuper, J., Wong, T.S., Roccatano, D., Wilmanns, M. & Schwaneberg, U. J Am Chem Soc. 2007 May 9;129(18):5786-7. Epub 2007 Apr 13. Europe PMC

Investigation of the ligand spectrum of human sterol carrier protein 2 using a direct mass spectrometry assay. Stanley, W.A., Versluis, K., Schultz, C., Heck, A.J. & Wilmanns, M. Arch Biochem Biophys. 2007 May 1;461(1):50-8. Epub 2007 Mar 15. Europe PMC

Octameric alcohol oxidase dissociates into stable, soluble monomers upon incubation with dimethylsulfoxide. Visser, N.V., Wang, D., Stanley, W.A., Groves, M.R., Wilmanns, M., Veenhuis, M. & van der Klei, I.J. Arch Biochem Biophys. 2007 Mar 15;459(2):208-13. Epub 2007 Jan 23. Europe PMC

On the routine use of soft X-rays in macromolecular crystallography. Part IV. Efficient determination of anomalous substructures in biomacromolecules using longer X-ray wavelengths. Mueller-Dieckmann, C., Panjikar, S., Schmidt, A., Mueller, S., Kuper, J., Geerlof, A., Wilmanns, M., Singh, R.K., Tucker, P.A. & Weiss, M.S. Acta Crystallogr D Biol Crystallogr. 2007 Mar;63(Pt 3):366-80. Epub 2007Feb 21. Europe PMC

A previously unobserved conformation for the human Pex5p receptor suggests roles for intrinsic flexibility and rigid domain motions in ligand binding. Stanley, W.A., Pursiainen, N.V., Garman, E.F., Juffer, A.H., Wilmanns, M. & Kursula, P. BMC Struct Biol. 2007 Apr 11;7:24. Europe PMC

Recognition of a functional peroxisome type 1 target by the dynamic import receptor pex5p. Stanley, W.A., Filipp, F.V., Kursula, P., Schuller, N., Erdmann, R., Schliebs, W., Sattler, M. & Wilmanns, M. Mol Cell. 2006 Dec 8;24(5):653-63. Europe PMC

Structural diversity in the six-fold redundant set of acyl-CoA carboxyltransferases in Mycobacterium tuberculosis. Holton, S.J., King-Scott, S., Nasser Eddine, A., Kaufmann, S.H. & Wilmanns, M. FEBS Lett. 2006 Dec 22;580(30):6898-902. Epub 2006 Nov 30. Europe PMC

Dynamic architecture of the peroxisomal import receptor Pex5p. A Stanley, W. & Wilmanns, M. Biochim Biophys Acta. 2006 Dec;1763(12):1592-8. Epub 2006 Nov 10. Europe PMC

Cellular target genes of Epstein-Barr virus nuclear antigen 2. Maier, S., Staffler, G., Hartmann, A., Hock, J., Henning, K., Grabusic, K., Mailhammer, R., Hoffmann, R., Wilmanns, M., Lang, R., Mages, J. & Kempkes, B. J Virol. 2006 Oct;80(19):9761-71. Europe PMC

The impact of protein characterization in structural proteomics. Geerlof, A., Brown, J., Coutard, B., Egloff, M.P., Enguita, F.J., Fogg, M.J., Gilbert, R.J., Groves, M.R., Haouz, A., Nettleship, J.E., Nordlund, P., Owens, R.J., Ruff, M., Sainsbury, S., Svergun, D.I. & Wilmanns, M. Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1125-36. Epub 2006Sep 19. Europe PMC

Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE. Alzari, P.M., Berglund, H., Berrow, N.S., Blagova, E., Busso, D., Cambillau, C., Campanacci, V., Christodoulou, E., Eiler, S., Fogg, M.J., Folkers, G., Geerlof, A., Hart, D., Haouz, A., Herman, M.D., Macieira, S., Nordlund, P., Perrakis, A., Quevillon-Cheruel, S., Tarandeau, F., van Tilbeurgh, H., Unger, T., Luna-Vargas, M.P., Velarde, M., Wilmanns, M. & Owens, R.J. Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1103-13. Epub 2006Sep 19. Europe PMC

The Ig doublet Z1Z2: a model system for the hybrid analysis of conformational dynamics in Ig tandems from titin. Marino, M., Zou, P., Svergun, D., Garcia, P., Edlich, C., Simon, B., Wilmanns, M., Muhle-Goll, C. & Mayans, O. Structure. 2006 Sep;14(9):1437-47. Europe PMC

Evidence for a dimeric assembly of two titin/telethonin complexes induced by the telethonin C-terminus. Pinotsis, N., Petoukhov, M., Lange, S., Svergun, D., Zou, P., Gautel, M. & Wilmanns, M. J Struct Biol. 2006 Aug;155(2):239-50. Epub 2006 Apr 27. Europe PMC

The Mycobacterium tuberculosis LipB enzyme functions as a cysteine/lysine dyad acyltransferase. Ma, Q., Zhao, X., Eddine, A.N., Geerlof, A., Li, X., Cronan, J.E., Kaufmann, S.H. & Wilmanns, M. Proc Natl Acad Sci U S A. 2006 Jun 6;103(23):8662-7. Epub 2006 May 30. Europe PMC

The structure of the 2[4Fe-4S] ferredoxin from Pseudomonas aeruginosa at 1.32-A resolution: comparison with other high-resolution structures of ferredoxins and contributing structural features to reduction potential values. Giastas, P., Pinotsis, N., Efthymiou, G., Wilmanns, M., Kyritsis, P., Moulis, J.M. & Mavridis, I.M. J Biol Inorg Chem. 2006 Jun;11(4):445-58. Epub 2006 Apr 5. Europe PMC

Mechanical strength of the titin Z1Z2-telethonin complex. Lee, E.H., Gao, M., Pinotsis, N., Wilmanns, M. & Schulten, K. Structure. 2006 Mar;14(3):497-509. Europe PMC

Crystal structure of the PB1 domain of NBR1. Muller, S., Kursula, I., Zou, P. & Wilmanns, M. FEBS Lett. 2006 Jan 9;580(1):341-4. Epub 2005 Dec 19. Europe PMC

Palindromic assembly of the giant muscle protein titin in the sarcomeric Z-disk. Zou, P., Pinotsis, N., Lange, S., Song, Y.H., Popov, A., Mavridis, I., Mayans, O.M., Gautel, M. & Wilmanns, M. Nature. 2006 Jan 12;439(7073):229-33. Europe PMC

Protein crystallization for X-ray. Mueller-Dieckmann, H.-J., Weiss, M.S. & Wilmanns, M. In "Encyclopedic Reference of Genomics and Proteomics in Molecular Medicine", Ganten, D. & Ruckpaul, K., eds. (2006). Springer Verlag, Heidelberg

Human alpha-defensins neutralize anthrax lethal toxin and protect against its fatal consequences. Kim, C., Gajendran, N., Mittrucker, H.W., Weiwad, M., Song, Y.H., Hurwitz, R., Wilmanns, M., Fischer, G. & Kaufmann, S.H. Proc Natl Acad Sci U S A 2005 Mar 29;102(13):4830-5. Epub 2005 Mar 16. Europe PMC

Regulation of the hetero-octameric ATP phosphoribosyl transferase complex from Thermotoga maritima by a tRNA synthetase-like subunit. Vega, M.C., Zou, P., Fernandez, F.J., Murphy, G.E., Sterner, R., Popov, A. & Wilmanns, M. Mol Microbiol 2005 Feb;55(3):675-86. Europe PMC

Two-fold repeated (betaalpha)(4) half-barrels may provide a molecular tool for dual substrate specificity. Kuper, J., Doenges, C. & Wilmanns, M. EMBO Rep 2005 Feb;6(2):134-9. Europe PMC

Molecular crystallography. Wilmanns, M. & Weiss, M.S. In "Encyclopedia of Condensed Matter Physics", Bassani, G., Liedl, G. & Wyder, P. (eds.), pp.453-458, Academic Press

Synergistic use of synchrotron radiation techniques for biological samples in solution: a case study on protein-ligand recognition by the peroxisomal import receptor Pex5p. Stanley, W.A., Sokolova, A., Brown, A., Clarke, D.T., Wilmanns, M. & Svergun, D.I. J Synchrotron Radiat 2004 Nov 1;11(Pt 6):490-6. Epub 2004 Oct 22. Europe PMC

Structural insights into the catalytic mechanism of phosphate ester hydrolysis by dUTPase. Barabas, O., Pongracz, V., Kovari, J., Wilmanns, M. & Vertessy, B.G. J Biol Chem 2004 Oct 8;279(41):42907-15. Epub 2004 Jun 17. Europe PMC

Combinatorial control of gene expression. Remenyi, A., Scholer, H.R. & Wilmanns, M. Nat Struct Mol Biol 2004 Sep;11(9):812-5. Europe PMC

Structural studies of the catalytic reaction pathway of a hyperthermophilic histidinol-phosphate aminotransferase. Fernandez, F.J., Vega, M.C., Lehmann, F., Sandmeier, E., Gehring, H., Christen, P. & Wilmanns, M. J Biol Chem 2004 Mar 8;. Europe PMC

Solvation in protein folding analysis: combination of theoretical and experimental approaches. Fernandez-Escamilla, A.M., Cheung, M.S., Vega, M.C., Wilmanns, M., Onuchic, J.N. & Serrano, L. Proc Natl Acad Sci U S A 2004 Mar 2;101(9):2834-9. Epub 2004 Feb 20. Europe PMC

Adaptation of Class-13 alpha-Amylases to Diverse Living Conditions. Linden, A. & Wilmanns, M. Chembiochem 2004 Feb 6;5(2):231-9. Europe PMC

Evolutionary markers in the (beta/alpha)(8)-barrel fold. Vega, M.C., Lorentzen, E., Linden, A. & Wilmanns, M. Curr Opin Chem Biol 2003 Dec;7(6):694-701. Europe PMC

Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers. Remenyi, A., Lins, K., Nissen, L.J., Reinbold, R., Scholer, H.R. & Wilmanns, M. Genes Dev 2003 Aug 15;17(16):2048-59. Europe PMC

OBF1 enhances transcriptional potential of Oct1. Lins, K., Remenyi, A., Tomilin, A., Massa, S., Wilmanns, M., Matthias, P. & Scholer, H.R. EMBO J 2003 May 1;22(9):2188-98. Europe PMC

Differential Regulation of a Hyperthermophilic alpha -Amylase with a Novel (Ca,Zn) Two-metal Center by Zinc. Linden, A., Mayans, O., Meyer-Klaucke, W., Antranikian, G. & Wilmanns, M. J Biol Chem 2003 Mar 14;278(11):9875-84. Europe PMC

Solution scattering suggests cross-linking function of telethonin in the complex with titin. Zou, P., Gautel, M., Geerlof, A., Wilmanns, M., Koch, M.H. & Svergun, D.I. J Biol Chem. 2003 Jan 24;278(4):2636-44. Epub 2002 Nov 20. Europe PMC

The TB structural genomics consortium: a resource for Mycobacterium tuberculosis biology. Terwilliger, T.C., Park, M.S., Waldo, G.S., Berendzen, J., Hung, L.W., Kim, C.Y., Smith, C.V., Sacchettini, J.C., Bellinzoni, M., Bossi, R., De Rossi, E., Mattevi, A., Milano, A., Riccardi, G., Rizzi, M., Roberts, M.M., Coker, A.R., Fossati, G., Mascagni, P., Coates, A.R., Wood, S.P., Goulding, C.W., Apostol, M.I., Anderson, D.H., Gill, H.S., Eisenberg, D.S., Taneja, B., Mande, S., Pohl, E., Lamzin, V., Tucker, P., Wilmanns, M., Colovos, C., Meyer-Klaucke, W., Munro, A.W., McLean, K.J., Marshall, K.R., Leys, D., Yang, J.K., Yoon, H.J., Lee, B.I., Lee, M.G., Kwak, J.E., Han, B.W., Lee, J.Y., Baek, S.H., Suh, S.W., Komen, M.M., Arcus, V.L., Baker, E.N., Lott, J.S., Jacobs W, J.r, Alber, T. & Rupp, B. Tuberculosis (Edinb) 2003;83(4):223-49. Europe PMC

Steered molecular dynamics studies of titin i1 domain unfolding. Gao, M., Wilmanns, M. & Schulten, K. Biophys J 2002 Dec;83(6):3435-45. Europe PMC

Structural conservation between the actin monomer-binding sites of twinfilin and actin-depolymerizing factor (ADF)/cofilin. Paavilainen, V.O., Merckel, M.C., Falck, S., Ojala, P.J., Pohl, E., Wilmanns, M. & Lappalainen, P. J Biol Chem 2002 Nov 8;277(45):43089-95. Europe PMC

Topography for independent binding of alpha-helical and PPII-helical ligands to a peroxisomal SH3 domain. Douangamath, A., Filipp, F.V., Klein, A.T., Barnett, P., Zou, P., Voorn-Brouwer, T., Vega, M.C., Mayans, O.M., Sattler, M., Distel, B. & Wilmanns, M. Mol Cell 2002 Nov;10(5):1007-17. Europe PMC

Differential activity by DNA-induced quarternary structures of POU transcription factors. Remenyi, A., Tomilin, A., Scholer, H.R. & Wilmanns, M. Biochem Pharmacol 2002 Sep;64(5-6):979-84. Europe PMC

Structural analysis of two enzymes catalysing reverse metabolic reactions implies common ancestry. Mayans, O., Ivens, A., Nissen, L.J., Kirschner, K. & Wilmanns, M. EMBO J 2002 Jul 1;21(13):3245-54. Europe PMC

The crystal structure of the allosteric non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeum Thermoproteus tenax. Pohl, E., Brunner, N., Wilmanns, M. & Hensel, R. J Biol Chem 2002 May 31;277(22):19938-45. Europe PMC

Unusual binding properties of the SH3 domain of the yeast actin-binding protein Abp1: structural and functional analysis. Fazi, B., Cope, M.J., Douangamath, A., Ferracuti, S., Schirwitz, K., Zucconi, A., Drubin, D.G., Wilmanns, M., Cesareni, G. & Castagnoli, L. J Biol Chem 2002 Feb 15;277(7):5290-8. Europe PMC

Stabilization of a ([beta][alpha])8-barrel protein by an engineered disulfide bridge. Ivens, A., Mayans, O., Szadkowski, H., Jurgens, C., Wilmanns, M. & Kirschner, K. Eur J Biochem 2002 Feb;269(4):1145-1153. Europe PMC

Integrated Structural Genomics in Europe Wilmanns, M. Gene Funct. Dis. 2002 3(1-2) 18-24

Structural Genomics in Europe and Beyond - Shifting Scientific Directions at EMBL Hamburg. Wilmanns, M. Acta Physica Polonica A, 101, 635-646

Crystallization of redox-insensitive Oct1 POU domain with different DNA-response elements. Remenyi, A., Pohl, E., Scholer, H.R. & Wilmanns, M. Acta Crystallogr D Biol Crystallogr 2001 Nov;57(Pt 11):1634-8. Europe PMC

Matti Saraste (1949-2001). Wilmanns, M. Structure. 2001 Sep;9(9):769-70. Europe PMC

Differential dimer activities of the transcription factor Oct-1 by DNA-induced interface swapping. Remenyi, A., Tomilin, A., Pohl, E., Lins, K., Philippsen, A., Reinbold, R., Scholer, H.R. & Wilmanns, M. Mol Cell 2001 Sep;8(3):569-80. Europe PMC

Divergent evolution of (betaalpha)8-barrel enzymes. Henn-Sax, M., Hocker, B., Wilmanns, M. & Sterner, R. Biol Chem 2001 Sep;382(9):1315-20. Europe PMC

Stability, catalytic versatility and evolution of the (beta alpha)(8)-barrel fold. Hocker, B., Jurgens, C., Wilmanns, M. & Sterner, R. Curr Opin Biotechnol 2001 Aug;12(4):376-81. Europe PMC

Structural evidence for a possible role of reversible disulphide bridge formation in the elasticity of the muscle protein titin. Mayans, O., Wuerges, J., Canela, S., Gautel, M. & Wilmanns, M. Structure (Camb) 2001 Apr 4;9(4):331-40. Europe PMC

Purification, characterization and crystallization of thermostable anthranilate phosphoribosyltransferase from Sulfolobus solfataricus. Ivens, A., Mayans, O., Szadkowski, H., Wilmanns, M. & Kirschner, K. Eur J Biochem 2001 Apr;268(8):2246-52. Europe PMC

Atomic resolution structure of a mutant of the spectrin SH3 domain. Berisio, R., Viguera, A., Serrano, L. & Wilmanns, M. Acta Crystallogr D Biol Crystallogr 2001 Feb;57(Pt 2):337-40. Europe PMC

Synergism with the coactivator OBF-1 (OCA-B, BOB-1) is mediated by a specific POU dimer configuration. Tomilin, A., Remenyi, A., Lins, K., Bak, H., Leidel, S., Vriend, G., Wilmanns, M. & Scholer, H.R. Cell 2000 Dec 8;103(6):853-64. Europe PMC

Structural evidence for evolution of the beta/alpha barrel scaffold by gene duplication and fusion. Lang, D., Thoma, R., Henn-Sax, M., Sterner, R. & Wilmanns, M. Science 2000 Sep 1;289(5484):1546-50. Europe PMC

Directed evolution of a (beta alpha)8-barrel enzyme to catalyze related reactions in two different metabolic pathways. Jurgens, C., Strom, A., Wegener, D., Hettwer, S., Wilmanns, M. & Sterner, R. Proc Natl Acad Sci U S A 2000 Aug 29;97(18):9925-30. Europe PMC

Activation of calcium/calmodulin regulated kinases. Wilmanns, M., Gautel, M. & Mayans, O. Cell Mol Biol (Noisy-le-grand) 2000 Jul;46(5):883-94. Europe PMC

Future structural biology applications with a free-electron laser - more than wild dreams? Wilmanns, M. J Synchrotron Radiat. 2000 Jan;7(Pt 1):41-6. Epub 2000 Jan 1. Europe PMC

Crystallization and preliminary X-ray diffraction analysis of the NAD- dependent non--phosphorylating GAPDH of the hyperthermophilic archaeon thermoproteus tenax. Brunner, N.A., Lang, D.A., Wilmanns, M. & Hensel, R. Acta Crystallogr D Biol Crystallogr 2000 Jan;56 (Pt 1):89-91 Europe PMC

The crystal structure of anthranilate synthase from Sulfolobus solfataricus: functional implications. Knochel, T., Ivens, A., Hester, G., Gonzalez, A., Bauerle, R., Wilmanns, M., Kirschner, K. & Jansonius, J.N. Proc Natl Acad Sci U S A 1999 Aug 17;96(17):9479-84 Europe PMC

Efficient expression, purification and crystallisation of two hyperthermostable enzymes of histidine biosynthesis. Thoma, R., Obmolova, G., Lang, D.A., Schwander, M., Jeno, P., Sterner, R. & Wilmanns, M. FEBS Lett 1999 Jul 2;454(1-2):1-6 Europe PMC

Thermodynamic analysis of alpha-spectrin SH3 and two of its circular permutants with different loop lengths: discerning the reasons for rapid folding in proteins. Martinez, J.C., Viguera, A.R., Berisio, R., Wilmanns, M., Mateo, P.L., Filimonov, V.V. & Serrano, L. Biochemistry. 1999 Jan 12;38(2):549-59. Europe PMC

Regulation von Proteinkinasen der MLCK-Familie. Wilmanns, M. & Gautel, M. Biospektrum 1999 5 198-201

X-ray analysis of protein crystals with thin plate morphology. Mayans, O. & Wilmanns, M. J. Synchr. Rad. 1999 6 1016-1020

Structural basis for activation of the titin kinase domain during myofibrillogenesis Mayans, O., van der Ven, P.F., Wilm, M., Mues, A., Young, P., Furst, D.O., Wilmanns, M. & Gautel, M. Nature 1998 Oct 29 395(6705) 863-869 Europe PMC

Crystal structure of the abl-SH3 domain complexed with a designed high-affinity peptide ligand: implications for SH3-ligand interactions. Pisabarro, M.T., Serrano, L. & Wilmanns, M. J Mol Biol 1998 Aug 21;281(3):513-21. Europe PMC

Different folding transition states may result in the same native structure. Viguera, A.R., Serrano, L. & Wilmanns, M. Nat Struct Biol 1996 Oct;3(10):874-80 Europe PMC

Structure of the binding site for inositol phosphates in a PH domain. Hyvonen, M., Macias, M.J., Nilges, M., Oschkinat, H., Saraste, M. & Wilmanns, M. EMBO J 1995 Oct 2;14(19):4676-85 Europe PMC

Inverse protein folding by the residue pair preference profile method: estimating the correctness of alignments of structurally compatible sequences. Wilmanns, M. & Eisenberg, D. Protein Eng 1995 Jul;8(7):627-39. Europe PMC

High-resolution crystal structures of tyrosine kinase SH3 domains complexed with proline-rich peptides. Musacchio, A., Saraste, M. & Wilmanns, M. Nat Struct Biol 1994 Aug;1(8):546-51. Europe PMC