Endstation P13 (Photo: EMBL / Photolab)

Endstation P13 (Photo: EMBL / Photolab)

Sample environment P13 (Photo: EMBL / Photolab)

Sample environment P13 (Photo: EMBL / Photolab)

Rolling proposals for beamtime can now be submitted via the EMBL user portal (SMIS).

P13 parameters as of 17 May 2017

  • Beam: P13 offers micro-beam conditions with a beam-size of 30 x 20 micron2 (H x V) and divergence below 0.15 mrad.  Typical crystal life-times are <1 min. for the focused beam (5 x 1012 ph/sec at 12 keV). By adjusting the shape of the adaptive KB-mirrors, the full beam can be defocused to a size of 150 x 70 micron2 at the sample position. Apertures can be used to collimate the beam down to a diameter of 10 micron.
  • Energy tunability: The beamline is tunable between 4.5 and 17.5 keV (2.7 Å to 0.7 Å).
  • Low energy: At 5 keV, the total flux is higher than 2 x 1012 ph/sec. Fluorescence spectra can be recorded with a AmpTek silicon drift detector. For data collection at energies lower than 5.5 keV, a Helium cone is available. Please contact your Local Contact if you intend to collect data at lower than 5.5 keV.
  • Diffractometer: The diffractometer is an MD2 instrument including a mini-kappa goniostat. The horizontal spindle-axis has a sphere of confusion of less than 2 micron when the mini-kappa goniostat is mounted. Three-click centering and 4D-scans are available.
  • Detector: PILATUS 6M (25 Hz, 450 micron sensor thickness) for shutter-less rotation data collection.
  • User Interface: The user interacts with the beamline via the Hamburg-version of mxCuBE v2 (ESRF) user interface.
  • Sample mounting: Crystals can be mounted manually. A MARVIN robot for mounting SPINE pins from a large capacity storage dewar (150 samples) is avaiiable; cycle time between two crystals is < 1 min.
  • Data processing: A 40 CPU linux-server is available for parallel data-processing with XDS. MOSFLM is installed.
  • Data storage and archival: Data will be stored on local servers for 2 months. At present, there are no facilities for data archival. Users are asked to bring their own back-up media (USB 2 or USB 3) for data archival.
  • Structure determination: The autoRickshaw and hkl2map pipelines for structure solution are available.
  • Ancillary facilities: A lab specifically designed and equipped for preparing derivatized crystals is accessible upon request. 42 elements in 150 compounds are available.

Typical experiments presently possible

  • Collection of native data on crystals with minimum dimension of 20 micron.
  • Maximum resolution achievable: 0.97 Å at detector edge (0.86  Å in the detector corner)  using 15 keV (0.827  Å) radiation.
  • Collection of highly redundant anomalous data using different kappa-settings.
  • Crystal characterization with beam sizes between 10 and 50 micron.
  • Typical duration of a full data collection: 1 min with focused beam (to reach lifetime limit of crystal).
  • Low-energy, long wavelength data collection with He-cone down to 4.5 keV upon request.
  • Full MAD-phasing capability

How to reference P13

The use of the EMBL beamlines must be acknowledged in all publications or presentations arising from data collection at the EMBL Hamburg unit. The following note is expected to appear in the acknowledgements, giving reference to the station(s) used, supporting beamline staff or any other assistance or information as appropriate, e.g. EU contract numbers, proposal numbers.

“The synchrotron <SAXS/MX> data was collected at beamline <P12/P13/P14> operated by EMBL Hamburg at the PETRA III storage ring (DESY, Hamburg, Germany). We would like to thank N.N.** for  the assistance in using the beamline.”

**If appropriate, names of the beamline staff in case they are not co-authors.

In case of P13, please cite the beamline paper: Cianci, Bourenkov, Pompidor et al. J.Synchr.Rad. 24:323-332 (OPEN ACCESS)

Contacts:

Beamline Scientist
Guillaume Pompidor
Tel: +49 (0) 40 89902-161
Email: g.pompidor@embl-hamburg.de

MX@PETRA3 coordinator
Thomas R. Schneider
Tel.:+49 (0) 40 89902-111