|Proposal submission for beamtime in 2017 has been closed on 23-Jan-2017. Submission of Rolling Proposals will be enabled in due course.|
Status of the beamline as of 13 Dec 2016
- Beam: P14 offers micro-beam conditions with a beam-size of 5 x 5 micron2 (V x H) and divergence below 0.3 mrad. For large crystals, the unfocused beam with a maximum size of 200 micron can be used. Moving between unfocused, half-focused, and fully focused beam conditions can be controlled easily via the user interface and takes less than 20 secs. Typical crystal life times are between 0.5 secs (5 x 5 micron2 beam with 5 x 1012 ph/sec @ 12 keV) and 2 min. (200 micron beam)
- Energy tunability: The beamline is tunable between 6 and 20 keV (2.1 and 0.6 A). Fluorescence spectra can be recorded with an AMPTEK silicon drift detector.
- Diffractometer: The diffractometer is an MD3 instrument with the spindle axis mounted in a vertical and downward configuration (including a mini-kappa goniostat). This instrument has a sphere of confusion smaller than 100 nm when the mini-kappa goniostat is mounted. Three-click centring and continuous '4D'-scans between two centring points are available.
- In situ datacollection: Diffraction data can be collected from crystals in CrystalDirectTM plates.
- Serial Crystallography: Serial Helical Line Scans are callable from an easy-to-use user interface.
- Detector: PILATUS 6M (25 Hz, 450 micron sensor thickness) for shutter-less oscillation data collection. From May 2017, an EIGER 16M detector will be available.
- User Interface: The user interacts with the beamline via the Hamburg-version of mxCuBE v2 (ESRF) user interface.
- Sample mounting: A MARVIN Sample Changer is available for automatic mounting of SPINE-pins from SPINE-pucks.
- Data processing: An 40 CPU linux-server is available for data-processing with XDS.
- Data storage and archival: Data will be stored on local servers for 1 month. At present, there are no facilities for data archival. Users are asked to bring their own back-up media (USB 2 or USB 3) for data archival.
- Structure determination: The autoRickshaw and hkl2map pipelines for structure solution are available.
- Ancillary facilities: A lab specifically designed and equipped for preparing derivatized crystals is accessible upon request.
Typical experiments presently possible
- Collection of native data on crystals with minimum dimension of 5 micron.
- Collection of high-multiplicity anomalous data sing different kappa-settings.
- Crystal characterization with beam sizes between 5 and 200 micron.
- Continuous helical data collection ('4d'-scan) on long crystals.
- Serial crystallography (see Gati et al. (2014) IUCrJ 1:87 (OPEN ACCESS)).
- 5/2017 Installation of EIGER 16M
- 2018 Installation of improved X-ray mirrors.
How to reference P14
The use of the EMBL beamlines must be acknowledged in all publications or presentations arising from data collection at the EMBL Hamburg unit. The following note is expected to appear in the acknowledgements, giving reference to the station(s) used, supporting beamline staff or any other assistance or information as appropriate, e.g. EU contract numbers, proposal numbers.
“The synchrotron <SAXS/MX> data was collected at beamline <P12/P13/P14> operated by EMBL Hamburg at the PETRA III storage ring (DESY, Hamburg, Germany). We would like to thank N.N.** for the assistance in using the beamline.”
**If appropriate, names of the beamline staff in case they are not co-authors.
Thomas R. Schneider
Tel.:+49 (0) 40 89902-111