Rolling proposals are now being accepted and will be reviewed on a regular basis throughout the year. To apply for beamtime, submit a proposal via the EMBL user portal

Status of the beamline as of 30 Oct 2018

  • Beam: P14 offers micro-beam conditions with a beam size of 5 x 10 micron2 (V x H) and divergence below 0.3 mrad. For large crystals, the collimated beam with a maximum size of 300 micron can be used. Moving between collimated and microfocusing beam conditions can be controlled easily via the user interface and takes less than 20 secs. Typical crystal life times are between 0.2 secs (5 x 10 micron2 beam with 1013 ph/sec @ 12 keV) and 2 mins (300 micron beam).
  • Energy tunability: The beamline is tunable between 6 and 20 keV (2.1 and 0.6 A). Fluorescence spectra can be recorded with an AMPTEK silicon drift detector.
  • Diffractometer: The diffractometer is an MD3 instrument with the spindle axis mounted in a vertical and downward configuration (including a mini-kappa goniostat). This instrument has a sphere of confusion smaller than 100 nm when the mini-kappa goniostat is mounted. Three-click centring and continuous '4D'-scans between two centring points are available.
  • In situ data collection: Diffraction data can be collected from crystals in CrystalDirectTM plates.
  • Serial Crystallography: Serial Helical Line Scans are callable from an easy-to-use user interface. Real-time filtering of diffraction images is available.
  • Detector: EIGER 16M (133 Hz, 750 Hz in 4M ROI mode).
  • User Interface: The user interacts with the beamline via the Hamburg version of mxCuBE v2 (ESRF) user interface.
  • Sample mounting: A MARVIN Sample Changer is available for automatic mounting of SPINE-pins from SPINE-pucks.
  • Data processing: Several 72 CPU linux servers are available for data processing with XDS or DIALS.
  • Data storage and archiving: Diffraction images are stored on a fast petabyte cluster storage system in gzip-compressed mini-cbf format. Instructions on how to download your data can be found on the data backup page.
  • Structure determination: The autoRickshaw and hkl2map pipelines for structure solution are available.
  • Ancillary facilities: A lab specifically designed and equipped for preparing derivatized crystals is accessible upon request.

Typical experiments presently possible

  • Collection of native data on crystals with minimum dimension of 5 microns.
  • Collection of high-multiplicity anomalous data using different kappa settings.
  • Crystal characterization with beam sizes between 5 and 200 microns.
  • Continuous helical data collection ('4d'-scan) on long crystals.
  • Serial crystallography (see Gati et al. (2014) IUCrJ 1:87 (OPEN ACCESS)).

Next steps

  • 2019 Installation of improved X-ray mirrors.
  • 2019 MD3 upgrade.

How to reference P14

The use of the EMBL beamlines must be acknowledged in all publications or presentations arising from data collection at the EMBL Hamburg unit. The following note is expected to appear in the acknowledgements, giving reference to the station(s) used, supporting beamline staff or any other assistance or information as appropriate, e.g. EU contract numbers, proposal numbers.

“The synchrotron <SAXS/MX> data was collected at beamline <P12/P13/P14> operated by EMBL Hamburg at the PETRA III storage ring (DESY, Hamburg, Germany). We would like to thank N.N.** for  the assistance in using the beamline.”

**If appropriate, names of the beamline staff in case they are not co-authors.


Beamline Scientist
Gleb Bourenkov
Tel: +49 (0) 40 89902-120

MX@PETRA3 coordinator
Thomas R. Schneider
Tel.:+49 (0) 40 89902-111