The call for 2018 beamtime at the EMBL beamlines is now open with the deadline on Friday 12th January 2018. From the beginning of December, users are welcome to apply for access to the SAXS P12 beamline - we specifically encourage mail-in applications using the robotic sample changer. Access to P12 for translational projects can also be financially supported by iNEXT using regular visits, mail-in and enhanced support modes.
EMBL Hamburg operates a synchrotron beamline P12 for small angle X-ray scattering (SAXS) at the PETRA III storage ring, under the coordination of Dmitri Svergun.
P12 is an undulator beamline utilising the wavelength range between 0.6 and 3 Å. The beamline is optimised for low background data collection from macromolecular solutions and kinetic experiments with high temporal resolution. The endstation is equipped with photon counting Pilatus and Eiger detectors and with a robotic sample changer. P12 allows for fully automated and remote data acquisition and processing using the Beamline Meta Server software.
Access to the beamline P12 is available to all research groups, and is prioritised on scientific grounds only. Project proposals for beamline access are evaluated by the Project Evaluation Committee (PEC). Please read HERE how to prepare an application for BioSAXS beamtime.
Further enquiries may be made to email@example.com.
How to reference P12
The use of the EMBL beamlines must be acknowledged in all publications or presentations arising from data collection at the EMBL Hamburg unit. The following note is expected to appear in the acknowledgements, giving reference to the station(s) used, supporting beamline staff or any other assistance or information, as appropriate, e.g. EU contract numbers, proposal numbers.
“The synchrotron <SAXS/MX> data was collected at beamline <P12/P13/P14> operated by EMBL Hamburg at the PETRA III storage ring (DESY, Hamburg, Germany). We would like to thank N.N.** for the assistance in using the beamline.”
**If appropriate, names of the beamline staff in case they are not co-authors.
In case of P12, please cite the beamline paper:
Blanchet CE, Spilotros A, Schwemmer F, Graewert MA, Kikhney A, Jeffries CM, Franke D, Mark D, Zengerle R, Cipriani F, Fiedler S, Roessle M, Svergun DI. (2015) J Appl Crystallogr. 48(2): 431-443. [Link to Open Access]
|Synchrotron source||Standard Petra U29 undulator|
Oxford double crystal monochromator, crystal Si (111) or Double Multilayer monochromator (DMM)
|Mirror||KB bimorph adaptative mirrors, Rh (-coated) and SiO3 (uncoated)|
|Control system||Beckhoff Twincat, TINE|
|Wavelength||0.06 – 0.3 nm (DCM), 0.08 -0.17 (DMM)|
|Focal spot||0.2 mm x 0.12 mm|
|Flux||1013 ph/s (DCM), 5*10)14 (DMM)|
|Slits||4*2 pairs: two sets of Cinel water cooled slits, defining slits: motorized highly polished tungsten blade, guard slits: Hybrid Scatterless slits (Xenocs)|
|Automated sample changer||2 x 96 wells (crystallization plates)
4 x 8 samples (200 µl PCR stripes) + 12 buffers (1.5 ml Eppendorf tubes)
temperature controlled storage (5-40°C)
FPLC/HPLC – Agilent BioInert system with autosampler
|Exposure unit||Quartz capillary, automated filling and rinsing, temperature controlled (5-55°C). Filling volume: 5 µl (20 µl for radiation sensitive samples)|
|Alternative sample environments||YZ- alignment stage for the positioning for user made sample cell/holder.
Temperature controlled (0-100°C) capillary holder (Ø 2mm).
Stopped flow SFM-400 (Bio-Logic)
|Detectors||Photon counting Pilatus 2M pixel detector (253 x 288 mm2), Pilatus 1M-W detector (423.6 x 70 mm2), Eiger 4M detector (155.2 x 162.5 mm2, 750 Hz frame rate))|
|Sample-detector distance||Tunable: 1.6 to 6.1 m|