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Small Angle X-Ray Scattering

Beamline X33 is a bending magnet beamline for biological small-angle scattering on the DORIS Fan D, located in the HASYLAB Hall 3. It uses a fixed wavelength of 1.5 Å. The endstation is equipped with a MAR Image Plate detector (345 mm2) and a photon counting Pilatus 1M pixel detector (67 x 420 mm2). The beamline is optimised for low background data collection from macromolecular solutions. A two-detector setup is available to simultaneously collect small and wide angle scattering (SAXS/WAXS) patterns.

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Contacts

Scientists in charge

Dmitri Svergun

Tel:
+49 40 89902-125

Manfred Roessle

Tel:
+49 40 89902-192

Small angle X-Ray scattering beamline X33

Tel:
+49 40 89902-153

Experimental possibilities

  • SAXS/WAXS experiments on macromolecules ranging from a few kDa to dozens MDa
  • Time resolved measurements and temperature scans
  • Automated liquid handling sample changer
  • Sample holders for solutions, gels, films, powders
  • Resolution range: 700 to 10 Å (Pilatus, Image Plate, SAXS); 25 to 6 Å (delay line gas detector, WAXS)
  • Typical exposure times: 30–180 s

Facts

Synchrotron source   DORIS bending magnet
Monochromator   Asymmetric cut 7° crystal Ge (111), horizontally focusing
Mirror   Rh coated, zerodur, vertically focussing
Acquisition system   CANBus, TINE
Wavelength   1.5 Å
Focal spot   2 mm x 0.6 mm
Flux   5 x 1011 ph/s/100mA
Slits   4 pairs, tungsten
Automated sample changer   2 x 96 samples (200 μl)
2 x 24 buffers (1.5 ml)
temperature controlled storage (5-40°C)
Vacuum cell   50 μl, polycarbonate windows, automated filling and rinsing, temperature controlled (5-50°C)
In-air cell   100 μl, mica windows, manual filling and rinsing – for viscose and solid samples
temperature controlled (0-80°C)
Detectors   photon counting Pilatus 1M pixel detector (67 x 420 mm2);
Mar345 image plate (345 mm2);
gas-filled delay line for WAXS (20 cm)
Sample-detector distance   Standard: 2,7 m (SAXS), 1.1 m (WAXS)

Recent changes, upgrades and developments

Roessle, M.W., Klaering, R., Ristau, U., Robrahn, B. Jahn, D., Gehrmann, T., Konarev, P. V., Round, A., Fiedler, S., Hermes, S. & Svergun, D. I. (2007). Upgrade of the Small Angle X-ray scattering Beamline X33 at the European Molecular Biology Laboratory, Hamburg. J. Appl. Crystallogr., 40, s190-s194.

Round, A.R., Franke, D., Moritz, S., Huchler, R., Fritsche, M., Malthan, D., Klaering, R., Svergun, D.I. & Roessle, M. (2008) Automated sample-changing robot for solution scattering experiments at the EMBL Hamburg SAXS station X33. J. Appl. Crystallogr., 41, 913-917.