EMBL Sample Preparation and Characterization Facility

 The Sample Preparation and Characterization facility (SPC) is located directly next to the EMBL beamlines at the PETRA III synchrotron. We offer a pipeline from the lab bench to our X-ray and SAXS beamlines, helping you to optimize and prepare your samples for structural studies.

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High Through-put Crystallization Facility

Our high-throughput crystallization laboratory offers initial crystallization screens from various suppliers as well as customized screens for optimization of initial hits, both suitable for soluble and membrane proteins. Drops are set with nanolitre dispensing robots; the TTP’s MOSQUITO equipped with an LCP (Lipidic Cubic Phase) module. Online observation of the plates is possible via the Crystallization Information Management System (CRIMS), which makes results available to users in real-time, along with all experimental parameters. We offer the CrystalDirect® technology, able to harvest protein crystals from 96-well CrystalDirectTM plates by means of laser photoablation.

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Molecular Biophysics Facility

Our protein characterization platform is located on the Center for Structural Systems Biology (CSSB) and connected to our HTX facility by a science bridge. It includes several biophysical instruments available for in-house and external users. We offer services for protein quality control in addition to characterization techniques for interaction of biomolecules. Techniques available at the SPC: MS MALDI coupled to a high mass detector, Thermofluor (TF), Circular Dichroism (CD), Fourier-transform infrared spectroscopy (FTIR), Isothermal Titration Calorimetry (ITC), nano Differential Scanning Fluorimetry (nDSF), Surface Plasmon Resonance (SPR), Biolayer interferometry (Octet), Microscale Thermophoresis (MST) and Dynamic Light Scattering (DLS).

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Access to Biological SAXS

 

We offer a direct access streamline to perform SAXS batch measurements. Please contact the SPC for information regarding schedule and sample preparation.
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Beamlines

Macromolecular Crystallography

http://www.embl-hamburg.de/services/mx/

 

Biological SAXS

https://www.embl-hamburg.de/biosaxs/