EMBO Practical Course
Protein Expression, Purification and Crystallisation
EMBL Hamburg, Germany Monday 23 August - Tuesday 31 August 2010
Programme
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All lectures in Seminar Room 48E |
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Monday 23rd August |
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09.30 - 09.45 |
Welcome |
Jochen Müller-Dieckmann |
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09.45 - 11.00 |
Heterologous expression in E. coli |
Gunter Stier and Arie Geerlof |
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11.00 - 11.30 |
Coffee |
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11.30 - 12.30 |
Mammalian expression systems |
David Hacker |
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12.30 - 13.30 |
Sandwich Lunch (EMBL) |
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13.30 - 14.30 |
Co-expression of protein complexes (pro- and eukaryotic) |
Imre Berger |
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14.30 - 15.30 |
High throughput cloning methodologies and their application to HTP expression screening |
Renaud Vincentelli and Jerome Basquin |
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15.30 - 18:30 |
Student poster session (Seminar room 48E) |
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19:00 - late |
BBQ |
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Tuesday 24th August |
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09.00 - 12.00 |
Practical Session 1 |
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12.00 - 13.00 |
Indian Lunch (EMBL) |
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13.00 – 16.00 |
Practical Session 2 |
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16.00 – 16.30 |
Tea |
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16.30 – 19.30 |
Practical Session 3 |
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20:30 |
Dinner vouchers |
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Wednesday 25th August |
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09.00 - 10.00 |
Purification, characterization and crystal quality optimization of membrane proteins - how to go beyond the 7 A limit |
Preben Morth |
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10.00 - 10.30 |
Coffee |
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10.30 - 13.30 |
Practical Session 4 |
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13.30 - 14.30 |
Sandwich lunch (EMBL) |
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14.30 - 17.30 |
Practical Session 5 |
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17.30 - 18.00 |
Break |
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18.00 - 19.00 |
Expression, purification and crystallization of membrane proteins |
Gebhard Schertler |
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20.30 |
Dinner (Schweinske, Luruper Hauptstr. 164) |
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Thursday 26th August |
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09.00 - 10.00 |
Library based construct screening for soluble protein expression |
Darren Hart |
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10.00 - 13.00 |
Practical Session 6 |
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13.00 - 14.00 |
Pizza Lunch (EMBL) |
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14.00 - 15.00 |
Identification and quantitation of proteins by mass spectrometry |
Jeroen Krijgsveld |
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15.00 - 15.30 |
Tea break |
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15.30 - 18.30 |
Practical Session 7 |
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18.30 - 19.15 |
Sample characterization in a HT environment |
Joanne Nettleship |
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19.15 - 20.00 |
Microcalorimetry of protein-ligand interactions |
Heather Lamb |
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20.00 |
Speakers Dinner / Dinner vouchers |
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Friday 27th August |
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09.00 - 09.45 |
Linking chemical space with the proteome |
Anne-Claude Gavin |
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09.45 - 10.45 |
Physico-chemical foundations of crystallization |
Bernhard Rupp |
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10.45 - 11.15 |
Coffee break |
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11.15 - 12.15 |
Crystal growth |
Alex McPherson |
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12.15 - 13.15 |
Engineering proteins for enhanced crystallizability |
Zygmunt Derewenda |
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13.15 - 14.15 |
Sandwich Lunch (EMBL) |
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14.15 - 15.15 |
Interpretation of the crystallization drop results |
Jeroen Mesters |
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15.15 – 16.00 |
Tea break |
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16.00 – 19.00 |
Practical Session 8 |
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20.00 |
Dinner (Don Quixote, Osdorfer Landstr. 162A) |
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Saturday 28th August |
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10.00 - 13.00 |
Practical Session 9 |
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13.00 - 14.00 |
Brunch (DESY bistro) |
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14.00 |
Free afternoon |
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20.00 |
Dinner vouchers |
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Sunday 29th August |
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09.00 - 12.00 |
Practical Session 10 |
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12.00 – 12.45 |
I) The use of additives in optimization |
Joanne Yeh |
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12.45 - 13.30 |
Lunch (DESY bistro) |
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13.30 - 16.30 |
Practical Session 11 |
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16.30 - 19.30 |
Practical Session 12 |
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20.30 |
Dinner (Einstein, Bahrenfelder Chaussee 45) |
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Monday 30th August |
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09.00 - 12.00 |
Practical Session 13 |
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12.00 - 12.45 |
Principles and modern methods of protein purification |
Young-Hwa Song |
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12.45 - 13.30 |
Localization of proteins in an organism |
Paul Tucker |
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13.30 - 14.30 |
Sandwich Lunch (EMBL) |
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14.30 - 17.30 |
Practical Session 14 |
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17.30 - 18.15 |
Protein complex formation with single-molecule fluorescence |
Christian Huebner |
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18.15 – 19.30 |
Review of Practical sessions |
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20.00 |
BBQ |
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Tuesday 31st August |
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09.00 - 12.00 |
Practical Session 15 |
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12.00 - 13.00 |
Sandwich lunch (EMBL) |
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