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EMBO Practical Course

Solution Scattering from Biological Macromolecules

EMBL Hamburg, Germany Monday 25 October - Monday 1 November 2010

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Tentative programme

Day 1: Basics of small-angle scattering (Monday, 25 Oct)
  Chair: D. Svergun
 09:00 - 09:15  Introduction - D. Svergun
 09:15 - 10:30 Scattering of X-rays (basics) - P. Vachette
 10:30 - 11:00 Coffee break
 11:00 - 12:00 Scattering of neutrons (basics) - R. Willumeit
 12:30 - 13:30 Lunch on the DESY site
 14:00 - 17:00 Safety training and visiting beamlines at Doris and Petra-III
 17:00 - 17:30 Coffee break
  Chair: D. Svergun
 17:30 - 19:30 Students presentations (10 min each)
 20:00 - 22:00 Welcome dinner


Day 2: Data processing (Tuesday, 26 Oct)
  Chair: P. Vachette
 09:00 - 10:00 Data reduction and processing - M. Roessle
 10:00 - 10:45 Students presentations (10 min each)
 10:45 - 11:15 Coffee break
 11:15 - 12:00 Students presentations (10 min each)
 12:30 - 13:30 Lunch on the DESY site
  Chair: K. Brown
 14:00 - 14:45 Students presentations (10 min each)
 14:45 - 16:45 Data reduction and processing tutorial - P. Konarev, M. Petoukhov
 16:45 - 17:15 Coffee break
 17:15 - 20:00 Practical session (EMBL seminar room), measurements at X33
 20:00 Dinner on the DESY site


Day 3: Ab initio methods (Wednesday, 27 Oct)
  Chair: M. Roessle
 09:00 - 10:00 Ab initio methods: how do they work - D. Svergun
 10:00 - 10:30 Coffee break
 10:30 - 12:30 Ab initio programs tutorial - D. Franke, M. Petoukhov
 13:00 - 14:00 Lunch on the DESY site
  Chair: M. Petoukhov
 14:30 - 15:15 Shape determination: applications to proteins - K. Brown
 15:14 - 15:45 Coffee break
 15:45 - 20:00 Practical session (EMBL seminar room), measurements at X33
 20:00 Dinner on the DESY site


Day 4: Rigid body refinement (Thursday, 28 Oct)
  Chair: P. Bernado
 09:00 - 10:00 Rigid body refinement (basics) - D. Svergun
 10:00 - 10:30 Coffee break
 10:30 - 12:00 Rigid body refinement tutorial - P. Konarev, M. Petoukhov
 12:00 - 12:45 Rigid body modelling: applications to proteins - K. Brown
 13:00 - 14:00 Lunch on the DESY site
 14:00 Free afternoon / Social event
 20:00 Dinner on the DESY site (provided for those with no other plans)


Day 5: Mixtures and interacting systems (Friday, 29 Oct)
  Chair: L. Makowski
 09.00 - 10:15 Form and structure factors, interactions, modeling - J. Skov Pedersen
 10:15 - 10:45 Coffee break
 10:45 - 12:30 Analysis of mixtures: tutorial - P. Konarev
 13:00 - 14:00 Lunch on the DESY site
  Chair: J. Skov Pedersen
 14:30 - 15:30 Analysis of flexible systems - P. Bernado
 15:30 - 16:00 Coffee break
 16:00 - 20:00 Practical session (EMBL seminar room), measurements at X33
 20:00 Dinner on the DESY site


Day 6: Applications and related methods (Saturday, 30 Oct)
  Chair: B. Vestergaard
 09:00 - 09:45 Use of WAXS to study proteins - L. Makowski
 09:45 - 10:30 Complementary use of SAXS and SANS - J. Trewhella
 10:30 - 11:00 Coffee break
 11:00 - 12:00 Sample preparation and characterization around SAXS - R. Meijers
 12:30 - 13:30 Lunch on the DESY site
  Chair: J. Trewhella
 14:00 - 14:45 Fibrillation of proteins - B. Vestergaard
 14:45 - 15:15 Coffee break
 15:45 - 20:00 Practical session (EMBL seminar room), measurements at X33
 20:00 Dinner on the DESY site


Day 7: Future outlook (Sunday, 31 Oct)
  Chair: A. Kikhney
 09:00 - 09:45 Time resolved biological SAXS at SPring-8 – N. Yagi
 09:45 - 10:30 Validation of biomacromolecular structures – G. Kleywegt
 10:30 - 11:00 Coffee break
 11:00 - 12:00 Practical session (EMBL seminar room)
 12:30 - 13:30 Lunch on the DESY site
 14:00 - 16:40 Practical session (EMBL seminar room)
 16:40 - 17:00 Coffee break
  Chair: N. Yagi
 17:00 - 19:30 Participating students: presentation of results (15 min each)
 20:00 Course dinner


Day 8: General discussion (Monday, 1 Nov)
  Chair: H. Mertens
 09.00 - 11.00 Participating students: presentation of results (15 min each)
 11.00 - 11.30 Coffee break
 11.30 - 12.30 Participating students: presentation of results (15 min each)
 12:30 - 13:00 Results of SASQuest competition, concluding remarks
 13:30 - 14:30 Lunch on the DESY site
  Departure