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 | Terese Bergfors - |  |
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The crystallization drop is full of information for the person who knows what to look for. However, for the inexperienced observer, the interpretation of the phenomena in the crystallization drop is not always a straightforward process. While it is sometimes easy to recognize a crystal, what about all those other solid phases of proteins like oils, precipitates, spherulites, and gels? What does it mean if the protein "oils out"? What does phase separation look like and how does it affect the outcome of the experiment? How can you recognize a promising precipitate from a "bad" one? What are the best types of crystals to use as seeds?
This lecture will give present pictorial examples of the most commonly encountered results and discuss 1) how to recognize the different phenomena, and 2) what to do with them.
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