Contact  Intranet  Sitemap     EMBL Hamburg > Courses and Conferences   Introduction   Programme   Sponsors   Speakers   Organisers   Materials EMBO PEPC5 course - PRELIMINARY Programme Monday 28th August Room 109   09.30 - 09.45     Paul Tucker - Welcome     09.45 - 11.00     Gunter Stier - Heterologous expression in E. Coli     11.00 - 11.30     Coffee Break     11.30 - 12.30     Arie Geerlof - Improving the solubility of expressed proteins     12.30 - 13.30     Lunch (EMBL)     13.30 - 14.30     Renaud Vincentelli - High throughput cloning methodologies and their application to HTP expression screening     14.30 - 15.30     Ray Owens - Eukaryotic expression - the options     15.30 - late     Student poster session. With barbeque, drinks and snacks.     Tuesday 29th August Room 109   09.00 - 12.00     Practical Session 1     12.00 - 13.00     Lunch (EMBL)     13.00 - 16.00     Practical Session 2     16.00 - 16.30     Tea Break     16.30 - 19.30     Practical Session 3     20.30     Dinner: Tsien-Yen, Flurstrasse 226, Tel: 803579     Wednesday 30th August Room 109   09.00 - 10.00     Reinhard Grisshammer - Expression of membrane proteins     10.00 - 10.30     Coffee break     10.30 - 13.30     Practical Session 4     13.30 - 14.30     Lunch (DESY Bistro)     14.30 - 17.30     Practical Session 5     17.30 - 18.00     Tea Break     18.00 - 19.00     Jochen Müller-Dieckmann - Automation and data mining in high-throughput crystallization     20.30     Dinner:          Speakers: Landhaus Flottbek, Baron-Voght-Str. 179, Tel: 822741          Participants: Meal allowance will be provided     Thursday 31th August Room 109   09.00 - 10.00     Young-Hwa Song - Principles and modern methods of protein purification     10.15 - 13.15     Practical Session 6     13.15 - 14.00     Lunch (EMBL)     14.00 - 15.00     Alan Cooper - Microcalorimetry of Protein-Ligand Interactions     15.00 - 15.30     Tea break     15.30 - 18.30     Practical Session 7     18.45 - 19.30     Joanne Nettleship - The ThermoFluor method     19.30 - 20.15     Terese Bergfors - Interpretation of the crystalization drop results     20.00     Dinner: Don Quichotte, Osdorfer Landstr. 162c, Tel. 801304     Friday 1st September Room 109   09.00 - 10.00     Bernhard Rupp - Physico-chemical foundations of crystallization     10.00 - 10.45     Klaus Gewert - FTIR     10.45 - 11.15     Coffee Break     11.15 - 12.15     Joanne Yeh - Calorimetric Approaches to Characterizing Effects of Additives on Protein Crystallization     12.15 - 13.15     Andy Freer - Extraction, purification and crystallization of Membrane Proteins     13.15 - 14.15     Lunch (EMBL)     14.15 - 15.15     Alex McPherson - Crystal growth     15.15 - 15.30     Group Photo     15.30 - 16.00     Tea break     16.15 - 19.15     Practical Session 8     20.30     Dinner: Panthera Rodizio, Ditmar-Koel-Strasse 3, Tel: 3786370     Saturday 2nd September   08.30 - 09.00     Breakfast provided at EMBL     09.00 - 12.00     Practical Session 9     12.00 - 13.00     Lunch (DESY Bistro)     13.00     Free afternoon          Meal allowance will be provided     Sunday 3rd September Room 109   08.30 - 09.00     Breakfast provided at EMBL     09.00 - 12.00     Practical Session 10     12.15 - 13.00     Ehmke Pohl - Complementarity of structural techniques     13.00 - 14.00     Lunch (DESY Bistro)     14.00 - 17.00     Practical Session 11     17.15 - 20.15     Practical Session 12     20.45     Dinner: Block House, Waitzstr. 1 Tel: 8907273     Monday 4th September Room 109   09.00 - 12.00     Practical Session 13     12.00 - 13.45     Bernhard Rupp and Anja Wienecke - Microfluidics     13.45 - 14.30     Lunch (EMBL)     14.30 - 17.30     Practical Session 14     17.30 - 18.15     Joanne Nettleship - The use of Mass Spectrometry in Structural Biology     18.15 - 19.30     Review of Practical G Results/ Crystal Characterization     20.00     Dinner: Panetteria, Osdorfer Landstr. 4, Tel: 821131     Tuesday 5th September Room 109   09.00 - 12.00     Practical Session 15     12.15 - 13.00     Final discussion with lunch     Practical Sessions 5 groups of four students. Long practicals of two 3hr sessions on adjacent days. These three practicals (repeated 5 times !!!) are A. Prokaryotic Expression (Jeanne Perry, Gunter Stiern) B. Protein Purification (Joanne Yeh, Young-Hwa Song, Matthew Groves) C. Membrane Protein Preparation & Crystallisation (Reinhard Grisshammer, Andy Freer) Short practicals of one 3hr session. These nine practicals (repeated 5 times !!!) are D. Protein complex characterisation, ITC (Alan Cooper) E. Protein Characterisation. (DLS and MALDI-TOF) (Bernhard Rupp) F. Setting up samples for high-throughput crystallization (Jochen Müller-Dieckmann) G. Setting up samples for high density cell fermentation (Anni Linden) H. Vapour diffusion methods (Alex McPherson) I. Seeding and Batch methods for crystallization (Terese Bergfors) J. Crystallization in lipidic cubic phases (Joanne Yeh) K. Dialysis and free interface diffusion methods (Tassos Perrakis) L. HTP expression screening (Renaud Vincentelli, Arie Geerlof) The table below shows which practicals will be run in which time slots (1-15). Session/Group I II III IV V 1 F G I D J 2 L I G E K 3 A B C I G 4 A B C G F 5 H A B C I 6 I A B C H 7 D E A B C 8 E D A B C 9 C H D A B 10 C F E A B 11 G L F K L 12 B C J L A 13 B C K J A 14 J K H F E 15 K J L H D