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| Tetsuro Fujisawa - Time-Resolved Small-Angle X-ray Scattering | |
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Tetsuro Fujisawa
RIKEN Harima Institute, SPring-8 Center, Laboratory for Biometal Science, Hyogo, Japan
One of the key features for the use of synchroton scattering is to perform time-resolved experiments. Time-resolved SAXS experiment is the experiment where the reaction is started by various perturbations such as mixing solutions and sudden changes of temperatures and pressures, and then the time course of scatterings is recorded. In solution, several conformers are mixed and inevitably time-resolved experiments should deal with this problem.
Its advantage lies in that the change of the fraction of each component is predictable based on the reaction kinetics. Time-resolved SAXS together with independent source of information (such as CD or fluorescence kinetics) becomes very powerful, especially for the reactions with limited number of intermediates. One of the most successful examples is the study for the polypeptide collapse in the early stage of folding.
In collaboration with Dr. Satoshi Takahashi's group of Osaka University, we developed a rapid solution mixer for the submillisecond-resolved SAXS measurement, in which neutralizing pH with mixing solutions does the initiation of folding. The observed dynamics for several proteins with different topologies in terms of their compactness opened the new understanding of folding collapse. My talk covers the theoretical and experimental basics of time-resolved SAXS and our applications including our recent results of pressure jump experiments.
Date/time: Saturday, 28 October, 10:45
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