| Wednesday, February 28 - Day 1 |
| 09:00-13:00 |
| Arrival, sample storage, coffee and lunch at EMBL |
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Session 1: Introductions
|
| 13:00-13:45 |
| Introduction, goals of the workshop - Tassos Perrakis |
|
| 13:45-14:30 |
| Predicting crystallization - Bernhard Rupp |
|
| 14:30-15:10 |
| Introduction to the Fluidigm technology - Eve Marquis |
|
| 15:10-15:45 |
| Reductive lysine methylation for crystallization - Jon Grimes |
|
| 15:45-16;30 |
| Counter diffusion in µ-capillaries - Juan Garcia-Ruiz |
|
| 16:30-20:00 |
| Sample preparation and setup |
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| Thursday, March 1 - Day 2 |
| 09:00-12:00 |
| Continuation of sample preparation and setup |
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| 12:00-13:00 | Lunch |
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Session 2: Technologies
|
| 13:00-13:45 |
| Surface mutations as a tool for crystallization - Zygmunt Derewenda |
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| 13:45-14:15 |
| Routine HTP proteolysis for construct design - Tom Walter |
|
| 14:15-14:45 |
| Screening crystallization space: size matters - Ian Berry |
|
| 14:45-15:15 |
| µ-fluidics, µ-capillaries, n-drops: what a mess - Patrick Celie |
|
| 15:45-16:00 |
|
| 16:00-16:15 | Coffee |
| 16:15-16:45 |
| Imaging and analysis of µ-fluidic experiments - Anja Wienecke |
|
| 16:45-17:05 |
| Image processing in automated X-ray crystallography - Tilo Strutz |
|
| 17:05-17:35 |
| PIMS crystallization information data management - Ian Berry |
|
| 17:35-20:00 |
| Inspection of experiments |
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|
| Friday, March 2 - Day 3 |
| Session 3: Case studies
|
| 09:00-09:30 |
| HTP construct screening coupled with crystallization - Darren Hart |
|
| 09:30-09:45 |
| SAXS accidents and µ-capillaries - Tassos Perrakis |
|
| 09:45-10:00 |
| µ-fluidics virus success from Oxford - Jon Grimes |
|
| 10:00-10:15 |
| One protein, three species and nineteen space groups - Patrick Celie |
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| 10:15-10:45 | Coffee |
| 10:45-11:15 |
| Lysine methylation stories - Jon Grimes |
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| 12:00-13:30 | Lunch |
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Discussions session
|
| 13:30-16:00 |
| Topic 1: from µ-fluidics to real-life crystals |
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| |
| Topic 2: Seeding in smaller and bigger volumes |
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