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SPINE-2 / BioXhit practical workshop - Preliminary programme
Wednesday, February 28 - Day 1
  09:00-13:00  
Arrival, sample storage, coffee and lunch at EMBL

  Session 1: Introductions
  13:00-13:45  
Introduction, goals of the workshop - Tassos Perrakis
  13:45-14:30  
Predicting crystallization - Bernhard Rupp
  14:30-15:10  
Introduction to the Fluidigm technology - Eve Marquis
  15:10-15:45  
Reductive lysine methylation for crystallization - Jon Grimes
  15:45-16;30  
Counter diffusion in µ-capillaries - Juan Garcia-Ruiz
  16:30-20:00  
Sample preparation and setup
 

Thursday, March 1 - Day 2
  09:00-12:00  
Continuation of sample preparation and setup
  12:00-13:00Lunch  

  Session 2: Technologies
  13:00-13:45  
Surface mutations as a tool for crystallization - Zygmunt Derewenda
  13:45-14:15  
Routine HTP proteolysis for construct design - Tom Walter
  14:15-14:45  
Screening crystallization space: size matters - Ian Berry
  14:45-15:15  
µ-fluidics, µ-capillaries, n-drops: what a mess - Patrick Celie
  15:45-16:00  
Thermofluor - Tom Walter
  16:00-16:15Coffee  
  16:15-16:45  
Imaging and analysis of µ-fluidic experiments - Anja Wienecke
  16:45-17:05  
Image processing in automated X-ray crystallography - Tilo Strutz
  17:05-17:35  
PIMS crystallization information data management - Ian Berry
  17:35-20:00  
Inspection of experiments
 

Friday, March 2 - Day 3
  Session 3: Case studies
  09:00-09:30  
HTP construct screening coupled with crystallization - Darren Hart
  09:30-09:45  
SAXS accidents and µ-capillaries - Tassos Perrakis
  09:45-10:00  
µ-fluidics virus success from Oxford - Jon Grimes
  10:00-10:15  
One protein, three species and nineteen space groups - Patrick Celie
  10:15-10:45Coffee  
  10:45-11:15  
Lysine methylation stories - Jon Grimes
  12:00-13:30Lunch  

  Discussions session
  13:30-16:00  
Topic 1: from µ-fluidics to real-life crystals
    
Topic 2: Seeding in smaller and bigger volumes