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| EMBO PEPC6 course - Programme | |
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| Monday 1 September |
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Lectures in EMBL seminar room
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| 09.30 - 09.45 |
| Jochen Muller-Dieckmann - Welcome |
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| 09.45 - 11.00 |
| Gunter Stier - Heterologous expression in E. coli |
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| 11.00 - 11.30 | Coffee |
| 11.30 - 12.30 |
| Joop van den Heuvel - Baculovirus/eukaryotic expression. |
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| 12.30 - 13.30 | Lunch break (EMBL) |
| 13.30 - 14.30 |
| Renaud Vincentelli - High throughput cloning methodologies and their application to HTP expression screening |
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| 14.30 - 15.30 |
| Darren Hart - Library based construct screening for soluble protein expression |
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| 15.30 - 18:30 |
| Student poster session at X-FEL hall - |
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| 19:00 - late |
| Buffet at Billard-Café Village, Osdorfer Landstr. 7 |
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| Tuesday 2 September |
| 09.00 - 12.00 |
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| 12.00 - 13.00 | Lunch (EMBL) |
| 13.00 - 16.00 |
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| 16.00 - 16.30 | Tea |
| 16.30 - 19.30 |
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| 20.30 | Dinner vouchers |
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| Wednesday 3 September |
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Lectures in EMBL seminar room
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| 09.00 - 10.00 |
| Imre Berger - Co-expression (pro- and eukaryotic) |
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| 10.00 - 10.30 | Coffee break |
| 10.30 - 13.30 |
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| 13.30 - 14.30 | Lunch (DESY Bistro) |
| 14.30 - 17.30 |
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| 17.30 - 18.00 | Break |
| 18.00 - 19.00 |
| Reinhard Grisshammer - Expression of membrane proteins |
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| 20.30 | Dinner (Don Quixote, Osdorfer Landstr. 162A) |
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| Thursday 4 September |
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Lectures in EMBL seminar room
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| 09.00 - 10.00 |
| Young-Hwa Song - Principles and modern methods of protein purification |
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| 10.00 - 13.00 |
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| 13.00 - 14.00 | Lunch (EMBL) |
| 14.00 - 15.00 |
| Andy Freer - Extraction, purification and crystallization of Membrane Proteins |
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| 15.00 - 15.30 | Tea break |
| 15.30 - 18.30 |
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| 18.30 - 19.15 |
| Par Nordlund - Directed evolution for solubility |
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| 19.15 - 20.00 |
| Joanne Nettleship - Sample characterization in a HT environment |
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| 20.00 | Speakers Dinner (TBA) / Dinner vouchers |
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| Friday 5 September |
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Lectures in EMBL seminar room
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| 09.00 - 09.45 |
| Stephen Fullerton - Microcalorimetry of protein-ligand interactions? |
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| 09.45 - 10.45 |
| Bernhard Rupp - Physico-chemical foundations of crystallization |
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| 10.45 - 11.15 | Coffee break |
| 11.15 - 12.15 |
| Alex McPherson - Crystal growth |
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| 12.15 - 13.15 |
| Zygmunt Derewenda - Surface entropy reduction |
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| 13.15 - 14.15 | Lunch (EMBL) |
| 14.15 - 15.15 |
| Joanne Yeh - I) The use of additives in optimization. II) Non-detergent approaches in membrane protein studies |
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| 15.15 - 16.00 | Tea break |
| 16.00 - 19.00 |
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| 20.00 | Dinner (Gröninger, Ost West Strasse 47) |
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| Saturday 6 September |
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Breakfast for students in EMBL Coffee Room
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| 10.00 - 13.00 |
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| 13.00 - 14.00 |
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| 14.00 | Free afternoon |
| 20.00 | Vouchers for dinner will be provided (in the DESY Bistro) for those with no other plans |
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| Sunday 7 September |
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Breakfast for students in EMBL Coffee Room
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| 09.00 - 12.00 |
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| 12.00 - 12.45 |
| Anne-Claude Gavin - Linking chemical space with the proteome |
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| 12.45 - 13.30 | Lunch (DESY bistro) |
| 13.30 - 16.30 |
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| 16.30 - 19.30 |
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| 20.30 | Dinner (Restaurant Canton, Osdorfer Landstr. 218) |
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| Monday 8 September |
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Room 109
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| 09.00 - 12.00 |
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| 12:00 - 12:45 |
| Chris Oubridge - Preparation of large complexes |
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| 12.45 - 13.30 |
| Terese Bergfors - Interpretation of the crystallization drop results |
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| 13.30 - 14.30 | Lunch (EMBL) |
| 14.30 - 17.30 |
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| 17.30 - 18.15 |
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| 18.15 - 19.30 |
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| 20.00 | Dinner (Country Kitchens, Hemmingstedter Weg 2) |
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| Tuesday 9 September |
| 09.00 - 12.00 |
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| 12.00 - 13.00 |
| Final discussion and lunch (EMBL) |
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Practical Sessions
5 groups of four students.
The student groups are:
Group I
Sergio Galán Bartual, Charlotta Andersson, Magdalena Wianecka, Elisabeth Meulenbroek
Group II
Jennifer Raaf, Sandro Waltersperger, Maria Dimarogona, David Swainsbury
Group III
Gianluca Varetti, Viivi Majava, Karine Fernanda dos Santos, Abishek Tomar
Group IV
Yujia Zhai, Kjersti Lien, Emese Kónya, Ulrich Eckhard
Group V
Hillary Hoffman, Joana Barata, Nicole Bischoff, Hannes Uchtenhagen
Long practicals of two 3hr sessions on adjacent days.
These four practicals are
A. Prokaryotic Expression (Gunter Stier & Frank Lehmann) [Small lab]
B. Eukaryotic Expression (Joop van den Heuvel + Arie Geerlof) [Small lab. & cell culture room]
C. Protein Purification (Joanne Yeh & Young-Hwa Song) [New lab, first bay]
D. Membrane Protein Preparation & Crystallization (Reinhard Grisshammer & Andy Freer) [New lab, last bay]
Short practicals of one 3hr session.
These seven practicals are
E. Protein complex characterisation. ITC (Stephen Fullerton) [Big lab, right hand bay]
F. Protein Characterisation. (DLS and Thermofluor) (Joanne Nettleship & Xandra Kreplin) [Helgoland, 215]
G. Vapour diffusion methods (Alex McPherson) [Big. Lab, right or left hand bays]
H. Seeding and Batch methods for crystallization (Terese Bergfors) [Big lab, left hand bay]
I. Crystallization in lipidic cubic phases (Joanne Yeh & Xandra Kreplin) [Big lab, middle bay]
J. Dialysis and counter diffusion (Bernhard Rupp) [Helgoland, 215 + 216]
K. HTP expression screening (Renaud Vincentelli & Rajesh Singh) [New lab, middle bay]
The table below shows which practicals will be run in which time slots (1-15).
| Session/Group | I | II | III | IV | V | Date |
| 1 | F | E | K | G | I | Tue., Sep 2nd 9:00 |
| 2 | I | F | E | K | G | Tue., Sep 2nd 13:00 |
| 3 | A | B | C | D | K | Tue., Sep 2nd 16:30 |
| 4 | A | B | C | D | F | Wed., Sep 3rd 10:30 |
| 5 | K | A | B | C | D | Wed., Sep 3rd 14:30 |
| 6 | J | A | B | C | D | Thu., Sep. 4th 10:00 |
| 7 | D | K | A | B | C | Thu., Sep. 4th 15:30 |
| 8 | D | H | A | B | C | Fri., Sep. 5th 16:00 |
| 9 | C | D | H | A | B | Sat., Sep. 6th 10:00 |
| 10 | C | D | I | A | B | Sun., Sep. 7th 9:00 |
| 11 | G | I | F | H | J | Sun., Sep. 7th 13:30 |
| 12 | B | C | D | J | A | Sun., Sep. 7th 16:30 |
| 13 | B | C | D | E | A | Mon., Sep 8th 9:00 |
| 14 | H | J | G | F | E | Mon., Sep 8th 14:30 |
| 15 | E | G | J | I | H | Tue., Sep. 9th 9:00 |
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