Small-Angle Scattering and Biophysical Methods

Olwyn Byron
Glasgow University, UK

In this session I will describe in detail the principles underlying analytical ultracentrifugation and its utility in characterising biomacromolecular systems that are also being studied with small-angle scattering. This will be illustrated with a number of examples chosen from our work and from the literature (see below, for example) in which additional complementary biophysical methods are used (e.g. circular dichroism, NMR, hydrodynamic bead modelling and bioinformatics-based modelling).

Dempsey, B.R., A. Economou, S.D. Dunn, and B.H. Shilton (2002). The ATPase domain of SecA can form a tetramer in solution. J. Mol. Biol. 315 831-843.

Solovyova, A.S., M. Nöllmann, T.J. Mitchell, and O. Byron (2004). The solution structure and oligomerisation behaviour of two bacterial toxins: pneumolysin and perfringolysin. O. Biophys. J. 87 540-552.

Nöllmann, M., J. He, O. Byron, and W. Stark (2004). Solution structure of the Tn3 resolvase-crossover site synaptic complex. Molec. Cell 16 127-137.

Mattinen, M.-L., K. Paakkonen, T. Ikonen, J. Craven, T. Drakenberg, R. Serimaa, J. Waltho, and A. Annila (2002). Quaternary structure built from subunits combining NMR and small-angle x-ray scattering data. Biophys. J. 83 1177-1183.

Smolle, M., A.E. Prior, A.E. Brown, A. Cooper, O. Byron, and J.G. Lindsay (2006). A new level of architectural complexity in the human pyruvate dehydrogenase complex. J. Biol. Chem. 281 19772-19780.

Date/time: Friday, 24 October, 9:00