Full length:
4215 bp
Lac operator:
5-19
Shine-Delgarno sequence: 58-63
Start codon (ATG): 71
MAD gene (stuffer): 71-733
Multiple cloning side: 742-793
T7 terminator: 896-943
Spectinomycin/Streptomycin resistance gene: 1905-1117
CDF origin of replication: 2783-2045
LacI gene: 4075-2996
T7 promoter: 4199-4215
Composition:
1017 A; 1137 C; 1094 G; 967 T; 0 OTHER
Percentage: 24% A; 27% C; 26% G; 23% T; 0%OTHER
Molecular Weight (kDa): ssDNA: 1300.81 dsDNA: 2598.7
ORIGIN
1 GGGGAATTGT GAGCGGATAA CAATTCCCCT GTAGAAATAA TTTTGTTTAA
CTTTAATAAG
61 GAGATATACC ATGGCGGCGG CGGTTCGGAT GAACATCCAG ATGCTGCTGG
AGGCGGCCGA
121 CTATCTGGAG CGGCGGGAGA GAGAAGCTGA ACATGGTTAT GCCTCCATGT
TACCATACAA
181 AAACAAGGAC AGAGATGCCT TAAAACGGAG GAACAAATCC AAAAAGAATA
ACAGCAGTAG
241 CAGATCAACT CACAATGAAA TGGAGAAGAA TAGACGGGCT CATCTTCGCT
TGTGCCTGGA
301 GAAGTTGAAG GGGCTGGTGC CACTGGGACC CGAATCAAGT CGACACACTA
CGTTGAGTTT
361 ATTAACAAAA GCCAAATTGC ACATAAAGAA ACTTGAAGAT TGTGACAGAA
AAGCCGTTCA
421 CCAAATCGAC CAGCTTCAGC GAGAGCAGCG ACACCTGAAG AGGCAGCTGG
AGAAGCTGGG
481 CATTGAGAGG ATCCGGATGG ACAGCATCGG CTCCACCGTC TCCTCGGAGC
GCTCCGACTC
541 CGACAGGGAA GAAATCGACG TTGACGTGGA GAGCACGGAC TATCTCACAG
GTGATCTGGA
601 CTGGAGCAGC AGCAGTGTGA GCGACTCTGA CGAGCGGGGC AGCATGCAGA
GCCTCGGCAG
661 TGATGAGGGC TATTCCAGCA CCAGCATCAA GAGAATAAAG CTGCAGGACA
GTCACAAGGC
721 GTGTCTTGGT CTCTAACTAG TGGTACCGGA TCCGAATTCG AGCTCCGTCG
ACAAGCTTGC
781 GGCCGCACTC GAGTCTGGTA AAGAAACCGC TGCTGCGAAA TTTGAACGCC
AGCACATGGA
841 CTCGTCTACT AGCGCAGCTT AATTAACCTA GGCTGCTGCC ACCGCTGAGC
AATAACTAGC
901 ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAC
CTCAGGCATT
961 TGAGAAGCAC ACGGTCACAC TGCTTCCGGT AGTCAATAAA CCGGTAAACC
AGCAATAGAC
1021 ATAAGCGGCT ATTTAACGAC CCTGCCCTGA ACCGACGACC GGGTCATCGT
GGCCGGATCT
1081 TGCGGCCCCT CGGCTTGAAC GAATTGTTAG ACATTATTTG CCGACTACCT
TGGTGATCTC
1141 GCCTTTCACG TAGTGGACAA ATTCTTCCAA CTGATCTGCG CGCGAGGCCA
AGCGATCTTC
1201 TTCTTGTCCA AGATAAGCCT GTCTAGCTTC AAGTATGACG GGCTGATACT
GGGCCGGCAG
1261 GCGCTCCATT GCCCAGTCGG CAGCGACATC CTTCGGCGCG ATTTTGCCGG
TTACTGCGCT
1321 GTACCAAATG CGGGACAACG TAAGCACTAC ATTTCGCTCA TCGCCAGCCC
AGTCGGGCGG
1381 CGAGTTCCAT AGCGTTAAGG TTTCATTTAG CGCCTCAAAT AGATCCTGTT
CAGGAACCGG
1441 ATCAAAGAGT TCCTCCGCCG CTGGACCTAC CAAGGCAACG CTATGTTCTC
TTGCTTTTGT
1501 CAGCAAGATA GCCAGATCAA TGTCGATCGT GGCTGGCTCG AAGATACCTG
CAAGAATGTC
1561 ATTGCGCTGC CATTCTCCAA ATTGCAGTTC GCGCTTAGCT GGATAACGCC
ACGGAATGAT
1621 GTCGTCGTGC ACAACAATGG TGACTTCTAC AGCGCGGAGA ATCTCGCTCT
CTCCAGGGGA
1681 AGCCGAAGTT TCCAAAAGGT CGTTGATCAA AGCTCGCCGC GTTGTTTCAT
CAAGCCTTAC
1741 GGTCACCGTA ACCAGCAAAT CAATATCACT GTGTGGCTTC AGGCCGCCAT
CCACTGCGGA
1801 GCCGTACAAA TGTACGGCCA GCAACGTCGG TTCGAGATGG CGCTCGATGA
CGCCAACTAC
1861 CTCTGATAGT TGAGTCGATA CTTCGGCGAT CACCGCTTCC CTCATACTCT
TCCTTTTTCA
1921 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
TTGAATGTAT
1981 TTAGAAAAAT AAACAAATAG CTAGCTCACT CGGTCGCTAC GCTCCGGGCG
TGAGACTGCG
2041 GCGGGCGCTG CGGACACATA CAAAGTTACC CACAGATTCC GTGGATAAGC
AGGGGACTAA
2101 CATGTGAGGC AAAACAGCAG GGCCGCGCCG GTGGCGTTTT TCCATAGGCT
CCGCCCTCCT
2161 GCCAGAGTTC ACATAAACAG ACGCTTTTCC GGTGCATCTG TGGGAGCCGT
GAGGCTCAAC
2221 CATGAATCTG ACAGTACGGG CGAAACCCGA CAGGACTTAA AGATCCCCAC
CGTTTCCGGC
2281 GGGTCGCTCC CTCTTGCGCT CTCCTGTTCC GACCCTGCCG TTTACCGGAT
ACCTGTTCCG
2341 CCTTTCTCCC TTACGGGAAG TGTGGCGCTT TCTCATAGCT CACACACTGG TATCTCGGCT
2401 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTAAGCAAGA ACTCCCCGTT
CAGCCCGACT
2461 GCTGCGCCTT ATCCGGTAAC TGTTCACTTG AGTCCAACCC GGAAAAGCAC
GGTAAAACGC
2521 CACTGGCAGC AGCCATTGGT AACTGGGAGT TCGCAGAGGA TTTGTTTAGC
TAAACACGCG
2581 GTTGCTCTTG AAGTGTGCGC CAAAGTCCGG CTACACTGGA AGGACAGATT
TGGTTGCTGT
2641 GCTCTGCGAA AGCCAGTTAC CACGGTTAAG CAGTTCCCCA ACTGACTTAA
CCTTCGATCA
2701 AACCACCTCC CCAGGTGGTT TTTTCGTTTA CAGGGCAAAA GATTACGCGC
AGAAAAAAAG
2761 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CTGAACCGCT CTAGATTTCA
GTGCAATTTA
2821 TCTCTTCAAA TGTAGCACCT GAAGTCAGCC CCATACGATA TAAGTTGTAA
TTCTCATGTT
2881 AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA
AGGGCATCGG
2941 TCGAGATCCC GGTGCCTAAT GAGTGAGCTA ACTTACATTA ATTGCGTTGC
GCTCACTGCC
3001 CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC
AACGCGCGGG
3061 GAGAGGCGGT TTGCGTATTG GGCGCCAGGG TGGTTTTTCT TTTCACCAGT
GAGACGGGCA
3121 ACAGCTGATT GCCCTTCACC GCCTGGCCCT GAGAGAGTTG CAGCAAGCGG
TCCACGCTGG
3181 TTTGCCCCAG CAGGCGAAAA TCCTGTTTGA TGGTGGTTAA CGGCGGGATA
TAACATGAGC
3241 TGTCTTCGGT ATCGTCGTAT CCCACTACCG AGATGTCCGC ACCAACGCGC
AGCCCGGACT
3301 CGGTAATGGC GCGCATTGCG CCCAGCGCCA TCTGATCGTT GGCAACCAGC
ATCGCAGTGG
3361 GAACGATGCC CTCATTCAGC ATTTGCATGG TTTGTTGAAA ACCGGACATG
GCACTCCAGT
3421 CGCCTTCCCG TTCCGCTATC GGCTGAATTT GATTGCGAGT GAGATATTTA
TGCCAGCCAG
3481 CCAGACGCAG ACGCGCCGAG ACAGAACTTA ATGGGCCCGC TAACAGCGCG
ATTTGCTGGT
3541 GACCCAATGC GACCAGATGC TCCACGCCCA GTCGCGTACC GTCTTCATGG
GAGAAAATAA
3601 TACTGTTGAT GGGTGTCTGG TCAGAGACAT CAAGAAATAA CGCCGGAACA
TTAGTGCAGG
3661 CAGCTTCCAC AGCAATGGCA TCCTGGTCAT CCAGCGGATA GTTAATGATC
AGCCCACTGA
3721 CGCGTTGCGC GAGAAGATTG TGCACCGCCG CTTTACAGGC TTCGACGCCG
CTTCGTTCTA
3781 CCATCGACAC CACCACGCTG GCACCCAGTT GATCGGCGCG AGATTTAATC
GCCGCGACAA
3841 TTTGCGACGG CGCGTGCAGG GCCAGACTGG AGGTGGCAAC GCCAATCAGC
AACGACTGTT
3901 TGCCCGCCAG TTGTTGTGCC ACGCGGTTGG GAATGTAATT CAGCTCCGCC
ATCGCCGCTT
3961 CCACTTTTTC CCGCGTTTTC GCAGAAACGT GGCTGGCCTG GTTCACCACG
CGGGAAACGG
4021 TCTGATAAGA GACACCGGCA TACTCTGCGA CATCGTATAA CGTTACTGGT
TTCACATTCA
4081 CCACCCTGAA TTGACTCTCT TCCGGGCGCT ATCATGCCAT ACCGCGAAAG
GTTTTGCGCC
4141 ATTCGATGGT GTCCGGGATC TCGACGCTCT CCCTTATGCG ACTCCTGCAT
TAGGAAATTA
4201 ATACGACTCA CTATA