th EMBO Practical Course on Solution Scattering from Biological Macromolecules
October 26 — November 1, 2020
EMBL Hamburg, Germany
Organisers: Dmitri Svergun, Al Kikhney, Melissa Gräwert
The course aims at young biochemists/biophysicists and researchers active in related structural methods with little or no experience in solution scattering. The course will cover basics of SAXS/SANS, instrumentation, data collection, modelling techniques and complementary use with other methods. The students are encouraged to bring their own samples to perform synchrotron solution scattering experiments on-site and the results will be used for practical tutorials and discussed on the last day of the course.
The course is supported by BIOSAXS GmbH.
Single copies of the articles below can be downloaded and printed only for the reader's personal research and study.
Svergun, D.I., Koch, M.H.J., Timmins, P.A., May, R.P. (2013)
Small Angle X-Ray and Neutron Scattering from Solutions of Biological Macromolecules
Oxford University Press
Feigin, L.A. & Svergun, D.I. (1987)
Structure Analysis by Small-Angle X-Ray and Neutron Scattering
© Plenum Press / Springer
Jeffries, C.M., Graewert, M.A., Blanchet, C.E., Langley, D.B., Whitten A.E. and Svergun, D.I. (2016)
Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments
Nature Protocols 11, 2122-2153
Blanchet, C.E. & Svergun, D.I. (2013)
Small-angle X-ray scattering on biological macromolecules and nanocomposites in solution.
Annu. Rev. Phys. Chem. 64, 37-54
© Annual Reviews DOI
Mertens H.D.T. & Svergun D.I. (2010)
Structural characterization of proteins and complexes using small-angle X-ray solution scattering.
J. Struct. Biol. 172(1), 128-41
Kikhney, A.G. and Svergun, D.I (2015)
A practical guide to small angle X-ray scattering (SAXS) of flexible and intrinsically disordered proteins.
FEBS Lett. 589(19A), 2570-2577
Franke, D., Petoukhov, M.V., Konarev, P.V., Panjkovich, A., Tuukkanen, A., Mertens, H.D.T., Kikhney, A.G., Hajizadeh, N.R., Franklin, J.M., Jeffries, C.M. and Svergun, D.I. (2017)
ATSAS 2.8: a comprehensive data analysis suite for small-angle scattering from macromolecular solutions.
J. Appl. Cryst. 50, 1212-1225
Svergun D.I. (1992)
Determination of the regularization parameter in indirect-transform methods using perceptual criteria.
J. Appl. Crystallogr. 25, 495-503
Svergun, D. I. (1999)
Restoring low resolution structure of biological macromolecules from solution scattering using simulated annealing.
Biophys. J. 76, 2879-2886
Franke, D. and Svergun, D.I. (2009)
DAMMIF, a program for rapid ab-initio shape determination in small-angle scattering.
J. Appl. Cryst., 42, 342-346
Svergun, D.I., Petoukhov, M.V. & Koch, M.H.J. (2001)
Determination of domain structure of proteins from X-ray solution scattering.
Biophys. J. 80, 2946-2953
Svergun D.I., Barberato C., & Koch M.H.J. (1995)
CRYSOL - a program to evalate X-ray solution scattering of biological macromolecules from atomic coordinates.
J. Appl. Crystallogr. 28, 768-773
Petoukhov, M.V. & Svergun, D.I. (2005)
Global rigid body modelling of macromolecular complexes against
small-angle scattering data.
Biophys. J. 89, 1237-1250
Tria, G., Mertens, H. D. T., Kachala, M. & Svergun, D. I. (2015)
Advanced ensemble modelling of flexible macromolecules using X-ray solution scattering. IUCrJ 2, 207-217
Valentini, E., Kikhney, A.G., Previtali, G., Jeffries, C.M. and Svergun D.I. (2014)
SASBDB, a repository for biological small-angle scattering data
Nucleic Acids Res. 43(D1), D357-D363
Kikhney, A.G., Panjkovich, A., Sokolova, A.V. & Svergun, D.I. (2016)
DARA: a web server for rapid search of structural neighbours using solution small angle X-ray scattering data.
Bioinformatics 32(4), 616-618