Figure 1: Crystal structure of the oligopeptide transporter PepTSt in the inward open conformation at 2.0 Å resolution. Bound lipids and water molecules are illustrated.
The Löw group uses innovative structural and biochemical tools to enhance the understanding of substrate and drug recognition in nutrient transporters.
Previous and current research
Cell membranes compartmentalise metabolic processes and serve as selective barriers for permeation. Therefore, nutrient transport through the plasma membrane is essential to maintain homeostasis within the cell. Many proton-coupled secondary active transporters of the major facilitator superfamily (MFS) are involved in the accumulation of nutrients above extracellular levels. Structural and functional analyses of MFS transporters suggest an alternating-access mechanism for the transport of substrates across the membrane. Here the transporter adopts different conformational states, allowing the substrate binding site to face either side of the membrane. Since MFS transporters are found in all branches of life and often with numerous gene copies, we believe that many if not all of these transporters follow a common transport mechanism.
Proton-coupled oligopeptide transporters of the PepT family (also known as the POT family) are responsible for the uptake of a range of different di- and tripeptides, derived from the digestion of dietary proteins, and are highly conserved in all kingdoms of life. The best studied members of this family include the two human peptide transporters, PepT1 and PepT2. Both are also of great pharmacological and pharmaceutical interest as they accept a number of drugs and amino acid-conjugated prodrugs as substrates. A detailed understanding of the structural basis for substrate recognition can therefore help to convert pharmacologically active compounds into substrates for PepT1 and PepT2 and improve their absorption in the small intestine and subsequent distribution in the body. We therefore study the proton-dependent oligopeptide transporter (POT) family using a combination of biochemical and biophysical methods.
Future projects and goals
- Characterisation of pro- and eukaryotic nutrient transporters in various states of the transport cycle using X-ray crystallography and/or cryo-EM to decipher a common transport mechanism of MFS transporters.
- Structural and dynamic insights into the binding mode of POTs to peptides, drugs, and inhibitors.
- Insights into structure and function of transport regulators of nutrient transporters by structural systems biology approaches.
Integral membrane proteins are a challenging class of proteins in terms of their structural and functional characterisation. Over the years we have developed and established new tools and a workflow for protein production and quality control of membrane proteins including functional assays. Furthermore, we are developing new ways to stabilise integral membrane proteins in vitro upon extraction from their natural environment.