EMBL Hamburg c/o DESY, Notkestraße 85, 22607 Hamburg, Germany


Total: 51 publication(s)


Shtykova EV, Dadinova LA, Fedorova NV, Golanikov AE, Bogacheva EN, Ksenofontov AL, Baratova LA, Shilova LA, Tashkin VY, Galimzyanov TR, Jeffries CM, Svergun DI, Batishchev OV. (2017)

Influenza virus Matrix Protein M1 preserves its conformation with pH, changing multimerization state at the priming stage due to electrostatics.

Sci Rep 7(1) doi: 10.1038/s41598-017-16986-y
Europe PMC | doi

Shtykova, EV., Bogacheva, EN, Dadinova, LA, Jeffries, CM, Fedorova, NV, Golovko, AO, Baratova, LA, Batishchev, OV. (2017)

Small-angle X-Ray analysis of macromolecular structure: the structure of protein NS2 (NEP) in solution

Crystallogr. Rep. 62(6):894-902. doi: 10.1134/S1063774517060220

Bhati M, Llamosas E, Jacques DA, Jeffries CM, Dastmalchi S, Ripin N, Nicholas HR, Matthews JM. (2017)

Interactions between LHX3- and ISL1-family LIM-homeodomain transcription factors are conserved in Caenorhabditis elegans.

Sci Rep 7(1):4579. doi: 10.1038/s41598-017-04587-8
Europe PMC | doi

Mauricio RP, Jeffries CM, Svergun DI, Deane JE. (2017)

The Shigella Virulence Factor IcsA Relieves N-WASP Autoinhibition by Displacing the Verprolin Homology/Cofilin/Acidic (VCA) Domain.

J. Biol. Chem. 292(1):134-145. doi: 10.1074/jbc.m116.758003
Europe PMC | doi

Franke D, Petoukhov MV, Konarev PV, Panjkovich A, Tuukkanen A, Mertens HDT, Kikhney AG, Hajizadeh NR, Franklin JM, Jeffries CM, Svergun DI. (2017)

ATSAS 2.8: a comprehensive data analysis suite for small-angle scattering from macromolecular solutions

J Appl Crystallogr 50(4):1212-1225. doi: 10.1107/S1600576717007786
Europe PMC | doi


Jeffries CM, Graewert MA, Blanchet CE, Langley DB, Whitten AE, Svergun DI. (2016)

Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments.

Nat Protoc 11(11):2122-2153. doi: 10.1038/nprot.2016.113
Europe PMC | doi

Dadinova LA, Shtykova EV, Konarev PV, Rodina EV, Snalina NE, Vorobyeva NN, Kurilova SA, Nazarova TI, Jeffries CM, Svergun DI.. (2016)

X-Ray Solution Scattering Study of Four Escherichia coli Enzymes Involved in Stationary-Phase Metabolism

PLoS ONE 11(5):e0156105. doi: 10.1371/journal.pone.0156105
Europe PMC | doi

Rubio-Marrero EN, Vincelli G, Jeffries CM, Shaikh TR, Pakos I, Ranaivoson FM, von Daake S, Demeler B, De Jaco A, Perkins G, Ellisman MH, Trewhella J, Comoletti D. (2016)

Structural characterization of the extracellular domain of CASPR2 and insights into its association with the novel ligand Contactin1.

J Biol Chem 291(11):5788-5802. doi: 10.1074/jbc.M115.705681
Europe PMC | doi

Baker MAB, Hynson RMG, Ganuelas LA, Mohammadi NS, Liew CW, Rey AA, Duff AP, Whitten AE, Jeffries CM, Delalez NJ, Morimoto YV, Stock D, Armitage JP, Turberfield AJ, Namba K, Berry RM, Lee LK.. (2016)

Domain-swap polymerization drives the self-assembly of the bacterial flagellar motor

Nat Struct Mol Biol 23(3):197-203. doi: 10.1038/nsmb.3172
Europe PMC | doi

Sun G, Nematollahi A, Nadvi NA, Kwan AH, Jeffries CM, Church W. (2016)

Expression, purification and crystallization of human Kynurenine Aminotransferase 2 exploiting a highly optimized codon set.

Protein Expr. Purif. doi: 10.1016/j.pep.2016.01.004
Europe PMC | doi


Wood K, Jeffries CM, Knott RB, Sokolova A, Jacques DA, Duff AP. (2015)

Exploring the structure of biological macromolecules in solution using Quokka, the small angle neutron scattering instrument, at ANSTO.

Nucl Instrum Methods Phys Res A 798:44-51. doi: 10.1016/j.nima.2015.06.034

Graewert MA, Franke D, Jeffries CM, Blanchet CE, Ruskule D, Kuhle K, Flieger A, Schäfer B, Tartsch B, Meijers R, Svergun DI. (2015)

Automated pipeline for purification, biophysical and x-ray analysis of biomacromolecular solutions.

Sci Rep 5 doi: 10.1038/srep10734
Europe PMC | doi

Gruszka DT, Whelan F, Farrance OE, Fung HK, Paci E, Jeffries CM, Svergun DI, Baldock C, Baumann CG, Brockwell DJ, Potts JR, Clarke J. (2015)

Cooperative folding of intrinsically disordered domains drives assembly of a strong elongated protein.

Nat Commun 6 doi: 10.1038/ncomms8271
Europe PMC | doi

Franke D, Jeffries CM, Svergun DI. (2015)

Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra.

Nat Methods 12(5):419-422. doi: 10.1038/nmeth.3358
Europe PMC | doi

Blanchet CE, Spilotros A, Schwemmer F, Graewert MA, Kikhney A, Jeffries CM, Franke D, Mark D, Zengerle R, Cipriani F, Fiedler S, Roessle M, Svergun DI. (2015)

Versatile sample environments and automation for biological solution X-ray scattering experiments at the P12 beamline (PETRA III, DESY).

J Appl Crystallogr 48(Pt 2):431-443. doi: 10.1107/s160057671500254x
Europe PMC | doi

Jeffries CM, Graewert MA, Svergun DI, Blanchet CE. (2015)

Limiting radiation damage for high-brilliance biological solution scattering: practical experience at the EMBL P12 beamline PETRAIII

J Synchrotron Radiat 22(Pt 2):273-279. doi: 10.1107/S1600577515000375
Europe PMC | doi

Valentini E, Kikhney AG, Previtali G, Jeffries CM, Svergun DI. (2015)

SASBDB, a repository for biological small-angle scattering data

Nucleic Acids Res. 43(D1):D357-D363. doi: 10.1093/nar/gku1047
Europe PMC | doi

Jeffries CM, Svergun DI. (2015)

High-throughput studies of protein shapes and interactions by synchrotron small-angle x-ray scattering.

Methods Mol. Biol. 1261:277-301. doi: 10.1007/978-1-4939-2230-7_15
Europe PMC | doi


Dunne M, Mertens HD, Garefalaki V, Jeffries CM, Thompson A, Lemke EA, Svergun DI, Mayer MJ, Narbad A, Meijers R. (2014)

The CD27L and CTP1L Endolysins Targeting Clostridia Contain a Built-in Trigger and Release Factor

PLoS Pathog. 10(7):e1004228. doi: 10.1371/journal.ppat.1004228
Europe PMC | doi


Mokbel N, Ilkovski B, Kreissl M, Memo M, Jeffries CM, Marttila M, Lehtokari VL, Lemola E, Grönholm M, Yang N, Menard D, Marcorelles P, Echaniz-Laguna A, Reimann J, Vainzof M, Monnier N, Ravenscroft G, McNamara E, Nowak KJ, Laing NG, Wallgren-Pettersson C, Trewhella J, Marston S, Ottenheijm C, North KN, Clarke NF. (2013)

K7del is a common TPM2 gene mutation associated with nemaline myopathy and raised myofibre calcium sensitivity.

Brain 136(Pt 2):494-507. doi: 10.1093/brain/aws348
Europe PMC | doi

Shtykova EV, Baratova LA, Fedorova NV, Radyukhin VA, Ksenofontov AL, Volkov VV, Shishkov AV, Dolgov AA, Shilova LA, Batishchev OV, Jeffries CM, Svergun DI. (2013)

Structural analysis of influenza A virus matrix protein M1 and its self-assemblies at low pH

PLoS ONE 8(12):e82431. doi: 10.1371/journal.pone.0082431
Europe PMC | doi


Lu Y, Kwan AH, Jeffries CM, Guss JM, Trewhella J. (2012)

The motif of human cardiac myosin-binding protein C is required for its Ca2+-dependent interaction with calmodulin.

J. Biol. Chem. 287(37):31596-31607. doi: 10.1074/jbc.m112.383299
Europe PMC | doi

Hynson RM, Jeffries CM, Trewhella J, Cocklin S. (2012)

Solution structure studies of monomeric human TIP47/perilipin-3 reveal a highly extended conformation.

Proteins 80(8):2046-2055. doi: 10.1002/prot.24095
Europe PMC | doi

Taylor JE, Chow JY, Jeffries CM, Kwan AH, Duff AP, Hamilton WA, Trewhella J. (2012)

Calmodulin binds a highly extended HIV-1 MA protein that refolds upon its release.

Biophys. J. 103(3):541-549. doi: 10.1016/j.bpj.2012.06.042
Europe PMC | doi

Jeffries CM, Farrugia W, Ramsland PA. (2012)

Crystallography and Small-Angle Scattering of Carbohydrate–Protein Complexes and Glycoproteins.

In: Yuriev E, Ramsland PA, Structural Glycobiology. pp 3-27.

Jeffries CM, Trewhella J. (2012)

Small-Angle Scattering

In: Wall ME, Quantitative Biology: From Molecular to Cellular Systems. Chapman & Hall/CRC Mathematical and Computational Biology. Taylor and Francis. pp 113-152.


Bhati M, Lee C, Gadd MS, Jeffries CM, Kwan A, Whitten AE, Trewhella J, Mackay JP, Matthews JM. (2011)

Solution structure of the LIM-homeodomain transcription factor complex Lhx3/Ldb1 and the effects of a pituitary mutation on key Lhx3 interactions.

PLoS ONE 7(7) doi: 10.1371/journal.pone.0040719
Europe PMC | doi

Gadd MS, Bhati M, Jeffries CM, Langley DB, Trewhella J, Guss JM, Matthews JM. (2011)

Structural basis for partial redundancy in a class of transcription factors, the LIM homeodomain proteins, in neural cell type specification.

J. Biol. Chem. 286(50):42971-42980. doi: 10.1074/jbc.m111.248559
Europe PMC | doi

Jeffries CM, Lu Y, Hynson RM, Taylor JE, Ballesteros M, Kwan AH, Trewhella J. (2011)

Human cardiac myosin binding protein C: structural flexibility within an extended modular architecture.

J. Mol. Biol. 414(5):735-748. doi: 10.1016/j.jmb.2011.10.029
Europe PMC | doi

Lu Y, Kwan AH, Trewhella J, Jeffries CM. (2011)

The C0C1 fragment of human cardiac myosin binding protein C has common binding determinants for both actin and myosin.

J. Mol. Biol. 413(5):908-913. doi: 10.1016/j.jmb.2011.09.026
Europe PMC | doi

Li N, Yun P, Jeffries CM, Langley D, Gamsjaeger R, Church WB, Hunter N, Collyer CA. (2011)

The modular structure of haemagglutinin/adhesin regions in gingipains of Porphyromonas gingivalis.

Mol. Microbiol. 81(5):1358-1373. doi: 10.1111/j.1365-2958.2011.07768.x
Europe PMC | doi

Orlova A, Galkin VE, Jeffries CM, Egelman EH, Trewhella J. (2011)

The N-terminal domains of myosin binding protein C can bind polymorphically to F-actin.

J. Mol. Biol. 412(3):379-386. doi: 10.1016/j.jmb.2011.07.056
Europe PMC | doi

Zhai Q, Landesman MB, Chung HY, Dierkers A, Jeffries CM, Trewhella J, Hill CP, Sundquist WI. (2011)

Activation of the retroviral budding factor ALIX.

J. Virol. 85(17):9222-9226. doi: 10.1128/jvi.02653-10
Europe PMC | doi

Lu Y, Jeffries CM, Trewhella J. (2011)

Invited review: probing the structures of muscle regulatory proteins using small-angle solution scattering.

Biopolymers 95(8):505-516. doi: 10.1002/bip.21624
Europe PMC | doi

Johansen D, Jeffries CM, Hammouda B, Trewhella J, Goldenberg DP. (2011)

Effects of macromolecular crowding on an intrinsically disordered protein characterized by small-angle neutron scattering with contrast matching.

Biophys. J. 100(4):1120-1128. doi: 10.1016/j.bpj.2011.01.020
Europe PMC | doi


Comoletti D, Miller MT, Jeffries CM, Wilson J, Demeler B, Taylor P, Trewhella J, Nakagawa T. (2010)

The macromolecular architecture of extracellular domain of alphaNRXN1: domain organization, flexibility, and insights into trans-synaptic disposition.

Structure 18(8):1044-1053. doi: 10.1016/j.str.2010.06.005
Europe PMC | doi

Chow JY, Jeffries CM, Kwan AH, Guss JM, Trewhella J. (2010)

Calmodulin disrupts the structure of the HIV-1 MA protein.

J. Mol. Biol. 400(4):702-714. doi: 10.1016/j.jmb.2010.05.022
Europe PMC | doi

Cross AJ, Jeffries CM, Trewhella J, Matthews JM. (2010)

LIM domain binding proteins 1 and 2 have different oligomeric states.

J. Mol. Biol. 399(1):133-144. doi: 10.1016/j.jmb.2010.04.006
Europe PMC | doi


Claridge JK, Headey SJ, Chow JY, Schwalbe M, Edwards PJ, Jeffries CM, Venugopal H, Trewhella J, Pascal SM. (2009)

A picornaviral loop-to-loop replication complex.

J. Struct. Biol. 166(3):251-262. doi: 10.1016/j.jsb.2009.02.010
Europe PMC | doi


Jacques DA, Langley DB, Jeffries CM, Cunningham KA, Burkholder WF, Guss JM, Trewhella J. (2008)

Histidine kinase regulation by a cyclophilin-like inhibitor.

J. Mol. Biol. 384(2):422-435. doi: 10.1016/j.jmb.2008.09.017
Europe PMC | doi

Matthews JM, Bhati M, Craig VJ, Deane JE, Jeffries C, Lee C, Nancarrow AL, Ryan DP, Sunde M. (2008)

Competition between LIM-binding domains

BIOCHEM SOC T 36:1393-1397. doi: 10.1042/BST0361393

Whitten AE, Jeffries CM, Harris SP, Trewhella J. (2008)

Cardiac myosin-binding protein C decorates F-actin: implications for cardiac function.

Proc. Natl. Acad. Sci. U.S.A. 105(47):18360-18365. doi: 10.1073/pnas.0808903105
Europe PMC | doi

Jeffries CM, Whitten AE, Harris SP, Trewhella J. (2008)

Small-angle X-ray scattering reveals the N-terminal domain organization of cardiac myosin binding protein C.

J. Mol. Biol. 377(4):1186-1199. doi: 10.1016/j.jmb.2008.01.080
Europe PMC | doi


Chan J, Whitten AE, Jeffries CM, Bosanac I, Mal TK, Ito J, Porumb H, Michikawa T, Mikoshiba K, Trewhella J, Ikura M. (2007)

Ligand-induced conformational changes via flexible linkers in the amino-terminal region of the inositol 1,4,5-trisphosphate receptor.

J. Mol. Biol. 373(5):1269-1280. doi: 10.1016/j.jmb.2007.08.057
Europe PMC | doi


Jeffries CM, Graham SC, Stokes PH, Collyer CA, Guss JM, Matthews JM. (2006)

Stabilization of a binary protein complex by intein-mediated cyclization.

Protein Sci. 15(11):2612-2618. doi: 10.1110/ps.062377006
Europe PMC | doi


Pasco N, Baronian KB, Jeffries C, Webber J, Hay J. (2004)

MICREDOX (R) - development of a ferricyanide-mediated rapid biochemical oxygen demand method using an immobilised Proteus vulgaris biocomponent

BIOSENS BIOELECTRON 20:524-532. doi: 10.1016/j.bios.2004.02.016


Yang H, Carr PD, McLoughlin SY, Liu JW, Horne I, Qiu X, Jeffries CM, Russell RJ, Oakeshott JG, Ollis DL. (2003)

Evolution of an organophosphate-degrading enzyme: a comparison of natural and directed evolution.

Protein Eng. 16(2):135-145. doi: 10.1093/proeng/gzg013
Europe PMC | doi


Pasco N, Baronian K, Jeffries C, Hay J. (2000)

Biochemical mediator demand - a novel rapid alternative for measuring biochemical oxygen demand



Heikinheimo P, Goldman A, Jeffries C, Ollis DL. (1999)

Of barn owls and bankers: a lush variety of alpha/beta hydrolases

STRUCTURE 7:R141-R146.

Pasco N, Jeffries C, Davies Q, Downard AJ, Roddick-Lanzilotta AD, Gorton L. (1999)

Characterisation of a thermophilic L-glutamate dehydrogenase biosensor for amperometric determination of L-glutamate by flow injection analysis



Jeffries C, Pasco N, Baronian K, Gorton L. (1997)

Evaluation of a thermophile enzyme for a carbon paste amperometric biosensor: L-glutamate dehydrogenase