Location & dates EMBL Hamburg, Germany 7 - 9 Oct 2019
Deadlines Registration closed Abstract submission closed
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Programme

Programme

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Day 1 - Monday 7 October 2019
TimeSpeaker
11:00-12:45 Arrival and Registration with light refreshments
12:45-13:00 Welcome address: Christian Löw, EMBL Hamburg, Germany
13:00-15:00

Session 1 - Membrane mimetics for biochemistry and biophysics
Chair:
Franz Hagn, Technical University of Munich, Germany

13:00-13:30

Structure and Dynamics of Membrane Proteins in Nanodiscs
Ilia G. Denisov - University of Illinois at Urbana-Champaign, USA 

13:30-13:45

Circularizing a reconstituted nanodisc using a fusion construct
Frederik Tidemand, University of Copenhagen, Denmark

13:45-14:15

Evolving GPCRs for Enabling Structural and Functional Studies
Andreas Plückthun, University of Zurich, Switzerland

14:15-14:30

Stabilization of Native Membrane Protein Targets for Drug Discovery
Anass Jawhari - CALIXAR, France

14:30-15:00

Structure based Drug Design for GPCRs
Jana Broecker - Sosei Heptares, UK 

15.00-15:30

Coffee break and Meet the Speakers: Ilia G. Densiov, Jana Broecker, Michael Overduin, Yifan Cheng

15:30-17:30

Session 2 - Structure, dynamics and folding of MP in discs
Chair:
Ayyalusamy Ramamoorthy, University of Michigan, USA

15:30-16:00

Converging lipidomics, metabolomics, interactomics and proteomics within native nanodiscs
Michael Overduin - University of Alberta, Canada

16:00-16:15

Nanodisc insertion affects water mobility at intra-membrane protein hydration sites
Karim Fahmy - Helmholtz-Zentrum Dresden-Rossendorf, Germany

16:15-16:45

Co-translational f;olding of alpha helical membrane proteins in lipid bilayers
Paula Booth - King's College London, UK 

16:45-17:00

The mechanism of membrane insertion of the CLIC1 chloride channel
Jose Ortega-Rolden - University of Kent, UK

17:00-17:30

Covalently circularized nanodiscs for studies of membrane proteins in a stable near-native environment
Gerhard Wagner - Harvard Medical School, USA 

17:30-19:00 Poster session 1 (odd numbers)
19:00-20:00 Dinner
20:00-21:30 Session 3 - Membrane protein structure and biophysics
Chair:
Christian Löw, EMBL Hamburg, Germany
20:00-20:30

Small angle X ray scattering to study membrane protein solutions
Haydyn Mertens - EMBL Hamburg, Germany

20:30-20:45 Electron cryo-microscopy structure of the canonical TRPC4 ion channel reveals the binding site for its regulatory proteins
Deivanayagabarathy Vinayagam, Max-Planck Institute for Molecular Pysiology, Germany
20:45-21:15

Single particle cryo-EM of membrane proteins in lipid nanodiscs
Yifan Cheng - University of California, San Francisco, USA

21:15-23:00 Welcome Reception
Day 2 - Tuesday 8 October 2019
TimeSpeaker
08:15-08:45

Beamline and Electron Microscopy Facility Tours (optional: limited places available, pre-registration required)

09:00-10:30

Session 4 - Novel membrane tools for structural biology
Chair: Ilia G. Denisov - University of Illinois at Urbana-Champaign, USA

09:00-09:30

Shaping bacterial cells with genetics to produce membrane proteins
Bruno Miroux - Institut de Biologie Physico-Chimique, CNRS-Université de Paris, France 

09:30-09:45

Realtime single-molecule FRET analysis of the conformational cycle of multidrug transporter LmrP
Jelle Hendrix, Hasselt University, Belgium

09:45-10:15

The Salipro system for membrane proteins: Structures, Antibodies and Direct Extraction
Jens Frauenfeld - Salipro, Sweden

10:15-10:30

In meso in situ serial crystallography at synchrotron and XFEL
Chia-Ying Huang - Swiss Light Source/PSI, Switzerland

10:30-11:00

Coffee break and Meet the Speakers: Paula Booth, Bruno Miroux, Gerhard Wagner, Jens Frauenfeld

11:00-12:30

Session 5 - Scattering methods on membrane proteins
Chair:
Antoinette Killian - Utrecht University, The Netherlands

11:00-11:30

New Polymer-Based Nanodiscs for Membrane Biophysics
Sandro Keller - University of Kaiserslautern, Germany

11:30-11:45

Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs
Inokentijs Josts - University of Hamburg, Germany

11:45-12:15

Methods for interpreting small angle scattering scattering data from membrane proteins
Lise Arleth - University of Copenhagen, Denemar

12:15-12:30

Combining invisible detergents and SEC-SANS to study membrane protein structure in solution
Nicolai Johansen - University of Copenhagen, Denmark

12:30-13:30

Lunch

13:30-15:00

Poster Session 2 (even numbers)

15:00-16:30

Session 6 - Drug design and function I
Chair: Sandro Keller - University of Kaiserslautern, Germany

15:00-15:30

Crystal structure of CC chemokine receptor 2A provides insights for the design of selective antagonists
Gisela Schnapp - Boehringer Ingelheim, Germany

15:30-15:45

Structure of amyloid-ß(1- 42) tetramers and octamers reveal edge conductivity channels as a mechanism for membrane damage
Edward Puig - IRB Barcelona, Spain

15:45 - 16:15

Biophysics platform integration in  LeadXpro’s structure-based drug discovery pipeline
Nicolas Bocquet - leadXpro AG, Switzerland

16:15-16:30

Gating of a divalent cation channel by 100 kHz magic-angle spinning NMR in lipid bilayers
Marta Bonaccorsi - Centre de RMN à Très Hauts Champs, FRE 2034  CNRS  Lyon, France

16:30-17:00 Coffee break and Meet the Speakers: Sandro Keller, Lise Arleth, Gisela Schnapp, Nicolas Bocquet, Daniel Nietlispach, Russell Bishop
17:00-18:30

Session 7 - Membrane Protein Structural Biology
Chair: Russell Bishop - McMaster University, Canada

17:00-17:30 Solution NMR studies of membrane proteins embedded in a lipid bilayer using the saposin nanoparticle system
Daniel Nietlispach - University of Cambridge, UK
17:30-17:45 In situ NMR spectroscopy of bacterial outer membrane proteins
Johannes Thoma - University of Gothenburg, Sweden
17:45-18:00 Developing ApoA1 and ApoE4 nanodiscs as a tool for XFEL-based structural biology
Megan Shelby - Lawrence Livermore National Lab, USA
18:00-18:30

Single particle cryo-EM of membrane proteins
Stefan Raunser - Max-Planck-Institut für molekulare Physiologie, Germany

19:00-22:00 Conference Dinner (DESY bistro/canteen)
Day 3 - Wednesday 9 October 2019
TimeSpeaker
08:15-08:45 Beamline and Electron Microscopy Facility Tours (optional: limited places available, pre-registration required)
09:00-10:30 Session 8 - Production and reconstitution of MPs
Chair: Cristina Paulino - University of Groningen, The Netherlands
09:00-09:30 The use of Styrene Maleic Acid Co-polymer (SMALP) for membrane protein characterisation
Tim Dafforn - University of Birmingham, UK
09:30-09:45 Purification of Mycobacteria tuberculosis ESX-1 inner membrane core complex and its individual subunits
Ye Gao - Maastricht University, The Netherlands
09:45-10:15 Precursor-based selective methyl labelling of cell-free synthesized proteins
Volker Dötsch - Goethe University Frankfurt, Germany
10:15-10:30 Functional reconstitution of the 5-HT3 receptor
Uriel López-Sánchez - CNRS- Institut de Biologie Structurale, France
10:30-11:15

Coffee break and Meet the Speakers: Tim Dafforn, Volker Dötsch, Tzviya Zeev Ben Mordehai, Cristina Paulino

11:15-12.45 Session 9 - Structure of function of MPs I
Chair: Paula Booth - King's College London, UK
11:15-11:45 Factors influencing the solubilization of membrane proteins by SMA copolymers
Antoinette Killian - Utrecht University, The Netherlands 
11:45-12:00 Structure of SLC4 transporter reveals novel conformational changes for ion transport
Jiansen Jiang - National Institutes of Health (NIH), USA
12:00-12:30

Membrane protein enriched extracellular vesicles for structural characterisation on native membranes
Tzviya Zeev Ben Mordehai - Utrecht University, The Netherlands 


12:30-12:45
The ion-transporter NKCC1: characterization of the structure-function relationship
Corinne Portioli -Istituto Italiano di Tecnologia, Italy and Baylor College of Medicine, USA
12.45-13:45 Lunch
13:45-15:15

Session 10 - Structure of function of MPs II
Chair: Michael Overduin - University of Alberta, Canada

13:45-14:00 Poster Prize Award
14:00-14:30 cryo-EM in lipid nanodiscs:The flips and flops of a scramblase story
Cristina Paulino - University of Groningen, The Netherlands 
14:30-14:45 Functional determinants of a small protein repressor controlling a broadly conserved bacterial sensor kinase
Jing Yuan - Max Planck Institute for Terrestrial Microbiology, Germany
14:45-15:15 The multifunctional enzymology of PagP
Russell Bishop - McMaster University, Canada
15:15-15:30 Final Remarks and Farewell