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EMBL Hamburg Biological
Small Angle Scattering
BioSAXS

Data reduction and processing

Manfred Roessle

EMBL, Hamburg Outstation, Germany

This lecture will explain the initial steps of a solution SAXS experiments. After an introductory part of SAXS instrumentation, the different data reduction and processing steps will be discussed in detail:

  1. Recording of Small Angle Scattering (SAS) data
    • SAXS beamlines;
    • SAXS beamline optics;
    • detection of X-rays for SAXS;
    • automation.
  2. Primary Data Reduction
    • radial averaging;
    • normalization;
    • subtraction;
    • pipeline for data analysis.
  3. Initial data processing
    • Guinier approximation;
    • estimation of the molecular weight;
    • quality check of SAXS data.
Download presentation (PDF, 6MB)

Date/time: Tuesday, 26 October 2010, 9:00


  Last modified: September 5, 2012

© BioSAXS group 2012