EMBL Hamburg Biological
Small Angle Scattering

10th EMBO Practical Course on

Solution Scattering from Biological Macromolecules

— November 3, 2021
Organisers: Dmitri Svergun, Melissa Gräwert, Al Kikhney

The course aims at young biochemists/biophysicists and researchers active in related structural methods with little or no experience in solution scattering. The course will cover basics of SAXS, instrumentation, data collection, modelling techniques and complementary use with other methods. The students are encouraged to send their own samples for synchrotron solution scattering data collection; the results will be used for practical tutorials and discussed on the last day of the course.

BIOSAXS   The course is supported by BIOSAXS GmbH.


Recommended reading

Single copies of the articles below can be downloaded and printed only for the reader's personal research and study.
Books Svergun, D.I., Koch, M.H.J., Timmins, P.A., May, R.P. (2013)
Small Angle X-Ray and Neutron Scattering from Solutions of Biological Macromolecules
Oxford University Press
  Feigin, L.A. & Svergun, D.I. (1987)
Structure Analysis by Small-Angle X-Ray and Neutron Scattering © Plenum Press / Springer
Publication gudelines Trewhella et al. (2017)
2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update Acta Crystallogr D Struct Biol 73(9), 710-728 DOI
Reviews Gräwert, M.A. and Svergun, D.I. (2020)
A beginner's guide to solution small-angle X-ray scattering (SAXS) Biochem (Lond) 42(1), 36-42 DOI
  Jeffries, C.M., Gräwert, M.A., Blanchet, C.E., Langley, D.B., Whitten A.E. and Svergun, D.I. (2016)
Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments Nature Protocols 11, 2122-2153 DOI
  Blanchet, C.E. & Svergun, D.I. (2013)
Small-angle X-ray scattering on biological macromolecules and nanocomposites in solution. Annu. Rev. Phys. Chem. 64, 37-54 © Annual Reviews DOI
  Mertens H.D.T. & Svergun D.I. (2010)
Structural characterization of proteins and complexes using small-angle X-ray solution scattering. J. Struct. Biol. 172(1), 128-41 © Elsevier DOI
  Kikhney, A.G. and Svergun, D.I (2015)
A practical guide to small angle X-ray scattering (SAXS) of flexible and intrinsically disordered proteins. FEBS Lett. 589(19A), 2570-2577 DOI
ATSAS Manalastas-Cantos, K., Konarev, P.V., Hajizadeh, N.R., Kikhney, A.G., Petoukhov, M.V., Molodenskiy, D.S., Panjkovich, A., Mertens, H.D.T., Gruzinov, A., Borges, C., Jeffries, C.M., Svergun, D.I., Franke, D. (2021)
ATSAS 3.0: expanded functionality and new tools for small-angle scattering data analysis.
J. Appl. Cryst. 54, 343-355
GNOM Svergun D.I. (1992)
Determination of the regularization parameter in indirect-transform methods using perceptual criteria. J. Appl. Crystallogr. 25, 495-503
DAMMIN Svergun, D. I. (1999)
Restoring low resolution structure of biological macromolecules from solution scattering using simulated annealing. Biophys. J. 76, 2879-2886
DAMMIF Franke, D. and Svergun, D.I. (2009)
DAMMIF, a program for rapid ab-initio shape determination in small-angle scattering.
J. Appl. Cryst., 42, 342-346
GASBOR Svergun, D.I., Petoukhov, M.V. & Koch, M.H.J. (2001)
Determination of domain structure of proteins from X-ray solution scattering. Biophys. J. 80, 2946-2953
CRYSOL Svergun D.I., Barberato C., & Koch M.H.J. (1995)
CRYSOL - a program to evalate X-ray solution scattering of biological macromolecules from atomic coordinates. J. Appl. Crystallogr. 28, 768-773
SASREF Petoukhov, M.V. & Svergun, D.I. (2005)
Global rigid body modelling of macromolecular complexes against small-angle scattering data. Biophys. J. 89, 1237-1250
EOM Tria, G., Mertens, H. D. T., Kachala, M. & Svergun, D. I. (2015)
Advanced ensemble modelling of flexible macromolecules using X-ray solution scattering. IUCrJ 2, 207-217
SASBDB Kikhney A.G., Borges C.R., Molodenskiy D.S., Jeffries C.M., Svergun D.I. (2020)
SASBDB: Towards an automatically curated andvalidated repository for biological scattering data Protein Science 29(1); 66-75 DOI
DARA Kikhney, A.G., Panjkovich, A., Sokolova, A.V. & Svergun, D.I. (2016)
DARA: a web server for rapid search of structural neighbours using solution small angle X-ray scattering data. Bioinformatics 32(4), 616-618 DOI

  Last modified: June 9, 2021

© BioSAXS group 2021