10th EMBO Practical Course on
Solution Scattering from Biological Macromolecules
October 25 — November 3, 2021Online
The course aims at young biochemists/biophysicists and researchers active in related structural methods with little or no experience in solution scattering. The course will cover basics of SAXS, instrumentation, data collection, modelling techniques and complementary use with other methods. The students are encouraged to send their own samples for synchrotron solution scattering data collection; the results will be used for practical tutorials and discussed on the last day of the course.
BIOSAXS GmbH .
Recommended reading
Single copies of the articles below can be downloaded and printed only for the reader's personal research and study.
Books
Svergun, D.I., Koch, M.H.J., Timmins, P.A., May, R.P. (2013)
Feigin, L.A. & Svergun, D.I. (1987)Structure Analysis by Small-Angle X-Ray and Neutron Scattering
© Plenum Press / Springer
Publication gudelines
Trewhella et al. (2017)2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update
Acta Crystallogr D Struct Biol 73(9), 710-728
DOI
Reviews
Gräwert, M.A. and Svergun, D.I. (2020)A beginner's guide to solution small-angle X-ray scattering (SAXS)
Biochem (Lond) 42(1), 36-42
DOI
Jeffries, C.M., Gräwert, M.A., Blanchet, C.E., Langley, D.B., Whitten A.E. and Svergun, D.I. (2016)Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments
Nature Protocols 11, 2122-2153
DOI
Blanchet, C.E. & Svergun, D.I. (2013)Small-angle X-ray scattering on biological macromolecules and nanocomposites in solution.
Annu. Rev. Phys. Chem. 64, 37-54
© Annual Reviews DOI
Mertens H.D.T. & Svergun D.I. (2010)Structural characterization of proteins and complexes using small-angle X-ray solution scattering.
J. Struct. Biol. 172(1), 128-41
© Elsevier DOI
Kikhney, A.G. and Svergun, D.I (2015)A practical guide to small angle X-ray scattering (SAXS) of flexible and intrinsically disordered proteins.
FEBS Lett. 589(19A), 2570-2577
DOI
ATSAS
Manalastas-Cantos, K., Konarev, P.V., Hajizadeh, N.R., Kikhney, A.G., Petoukhov, M.V., Molodenskiy, D.S., Panjkovich, A., Mertens, H.D.T., Gruzinov, A., Borges, C., Jeffries, C.M., Svergun, D.I., Franke, D. (2021)ATSAS 3.0: expanded functionality and new tools for small-angle scattering data analysis .J. Appl. Cryst. 54, 343-355
GNOM
Svergun D.I. (1992)Determination of the regularization parameter in indirect-transform methods using perceptual criteria .
J. Appl. Crystallogr. 25, 495-503
DAMMIN
Svergun, D. I. (1999)Restoring low resolution structure of biological macromolecules from solution scattering using simulated annealing .
Biophys. J. 76, 2879-2886
DAMMIF
Franke, D. and Svergun, D.I. (2009)DAMMIF, a program for rapid ab-initio shape determination in small-angle scattering .J. Appl. Cryst. , 42, 342-346
GASBOR
Svergun, D.I., Petoukhov, M.V. & Koch, M.H.J. (2001)Determination of domain structure of proteins from X-ray solution scattering .
Biophys. J. 80, 2946-2953
CRYSOL
Svergun D.I., Barberato C., & Koch M.H.J. (1995)CRYSOL - a program to evalate X-ray solution scattering of biological macromolecules from atomic coordinates .
J. Appl. Crystallogr. 28, 768-773
SASREF
Petoukhov, M.V. & Svergun, D.I. (2005)Global rigid body modelling of macromolecular complexes against
small-angle scattering data .
Biophys. J. 89, 1237-1250
EOM
Tria, G., Mertens, H. D. T., Kachala, M. & Svergun, D. I. (2015)Advanced ensemble modelling of flexible macromolecules using X-ray solution scattering . IUCrJ 2, 207-217
SASBDB
Kikhney A.G., Borges C.R., Molodenskiy D.S., Jeffries C.M., Svergun D.I. (2020)SASBDB: Towards an automatically curated andvalidated repository for biological scattering data
Protein Science 29(1); 66-75
DOI
DARA
Kikhney, A.G., Panjkovich, A., Sokolova, A.V. & Svergun, D.I. (2016)DARA: a web server for rapid search of structural neighbours using solution small angle X-ray scattering data.
Bioinformatics 32(4), 616-618
DOI
