EMBL Hamburg Biological
Small Angle Scattering

WeNMR workshop on structure determination of biomolecular complexes

SAXS from Biological Macromolecules in Solution

March 18, 2013
Academia Sinica, Taipei, Taiwan

Official workshop page   Watch on YouTube.com


Part I

Small angle X-ray scattering from biological macromolecules: introduction (PDF, 3.3 MB)

SAXS data reduction and processing (PDF, 3.0 MB)

  • Experiment setup
  • Exposure and radial averaging
  • Notations and units
  • Data quality: radiation damage
  • Background subtraction
  • Data quality: aggregation
  • Dilution series
  • Inter-particle interactions, merging
  • Kratky plot

Primary data analysis: overall parameters

  • Radius of gyration (Rg)
  • Guinier plot
  • I(0), molecular mass
  • Porod law, porod volume
  • Distance distribution function p(r)

Part II

Ab initio shape determination (PDF, 2.5 MB)

  • Simple bodies
  • Dummy atom models
  • Dummy residue models
  • Model validity

Part III

Modelling against small angle scattering data (PDF, 1.5 MB)

  • Validation of atomic models
  • Rigid body modelling
  • Oligomeric mixtures
  • Flexible systems

  Last modified: November 1, 2017

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