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  Home  >  Courses  >  WeNMR' 2013 lecture

WeNMR workshop on structure determination of biomolecular complexes

SAXS from Biological Macromolecules in Solution

March 18, 2013
Academia Sinica, Taipei, Taiwan

Official workshop page   Watch on YouTube.com

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Small angle X-ray scattering from biological macromolecules: introduction (PDF, 3.3 MB)

 

SAXS data reduction and processing (PDF, 3.0 MB)

  • Experiment setup
  • Exposure and radial averaging
  • Notations and units
  • Data quality: radiation damage
  • Background subtraction
  • Data quality: aggregation
  • Dilution series
  • Inter-particle interactions, merging
  • Kratky plot

Primary data analysis: overall parameters

  • Radius of gyration (Rg)
  • Guinier plot
  • I(0), molecular mass
  • Porod law, porod volume
  • Distance distribution function p(r)

Ab initio shape determination (PDF, 2.5 MB)

  • Simple bodies
  • Dummy atom models
  • Dummy residue models
  • Model validity

Modelling against small angle scattering data (PDF, 1.5 MB)

  • Validation of atomic models
  • Rigid body modelling
  • Oligomeric mixtures
  • Flexible systems

  Last modified: December 4, 2013

© BioSAXS group 2013