WeNMR workshop on structure determination of biomolecular complexes

SAXS from Biological Macromolecules in Solution

Official workshop page   Watch on YouTube.com

Part I

Small angle X-ray scattering from biological macromolecules: introduction (PDF, 3.3 MB)

SAXS data reduction and processing (PDF, 3.0 MB)

• Experiment setup
• Exposure and radial averaging
• Notations and units
• Data quality: radiation damage
• Background subtraction
• Data quality: aggregation
• Dilution series
• Inter-particle interactions, merging
• Kratky plot

Primary data analysis: overall parameters

• Radius of gyration (R_g)
• Guinier plot
• I(0), molecular mass
• Porod law, porod volume
• Distance distribution function p(r)

Part II

Ab initio shape determination (PDF, 2.5 MB)

• Simple bodies
• Dummy atom models
• Dummy residue models
• Model validity

Part III

Modelling against small angle scattering data (PDF, 1.5 MB)

• Validation of atomic models
• Rigid body modelling
• Oligomeric mixtures
• Flexible systems