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EMBO Practical Course on

Solution Scattering from Biological Macromolecules

October 27 - November 3, 2014
EMBL Hamburg, Germany
Organisers: Dmitri Svergun, Al Kikhney, Maxim Petoukhov, Rob Meijers

Programme
Registration deadline: July 31, 2014
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The course aims at young biochemists/biophysicists and researchers active in related structural methods with little or no experience in solution scattering. The course will cover basics of SAXS/SANS, instrumentation, data collection, modelling techniques and complementary use with other methods. The students are encouraged to bring their own samples to perform synchrotron solution scattering experiments on-site and the results will be used for practical tutorials and discussed on the last day of the course.

The course is supported by BIOSAXS.

Abstracts of the talks and slides in PDF

Abstract	Scattering of X-rays (basics) (3MB) – Patrice Vachette
Abstract	Scattering of neutrons (basics) (14MB) – Cy Jeffries
	Biodeuteration and SANS facilities (0.05MB) – Cy Jeffries
Abstract	Sample preparation and characterisation (2MB) – Rob Meijers
Abstract	Data reduction and processing (3MB) – Al Kikhney
Abstract	Ab initio methods: how/why do they work (3MB) – Dmitri Svergun
Abstract	Rigid body refinement (basics) (2MB) – Dmitri Svergun
Abstract	Form and structure factors: modeling and interactions (3MB) – Jan Skov Pedersen
Abstract	Studies of flexible systems (28MB) – Pau Bernadó
	Analysis of mixtures: tutorial (10MB) – Haydyn Mertens
Abstract	Structural investigation of protein and peptide fibrillation (84MB) – Bente Vestergaard
Abstract	Joint Use of SAXS and SANS (10MB) – Jill Trewhella
	Joint use of SAXS and NMR (7MB) – Annalisa Pastore
	Membrane proteins: structural and functional challenges (40MB) – Susana Andrade
	Joint use of SAS and AUC (18MB) – Olwyn Byron
Abstract	Using SAXS to study DNA regulation (5MB) – Titia Sixma
	High brilliance SAXS on synchrotrons (4MB) – Manfred Roessle
	Microfluidic sample environments (43MB) – Bente Vestergaard
Abstract	Validation of biomacromolecular structures (9MB) – Gerard Kleywegt
	SASBDB: a curated repository for small angle scattering data (5MB) – Erica Valentini

Recommended reading

Single copies of the articles below can be downloaded and printed only for the reader's personal research and study.

Books	Svergun, D.I., Koch, M.H.J., Timmins, P.A., May, R.P. (2013) Small Angle X-Ray and Neutron Scattering from Solutions of Biological Macromolecules Oxford University Press
	Feigin, L.A. & Svergun, D.I. (1987) Structure Analysis by Small-Angle X-Ray and Neutron Scattering © Plenum Press / Springer
Reviews	Blanchet, C.E. & Svergun, D.I. (2013) Small-angle X-ray scattering on biological macromolecules and nanocomposites in solution. Annu. Rev. Phys. Chem. 64, 37-54 © Annual Reviews DOI
	Mertens H.D.T. & Svergun D.I. (2010) Structural characterization of proteins and complexes using small-angle X-ray solution scattering. J. Struct. Biol. 172(1), 128-41 © Elsevier DOI
	Svergun, D.I. (2007) Small-angle scattering studies of macromolecular solutions. J. Appl. Cryst. 40, s10-s17
ATSAS	Petoukhov, M.V., Franke, D., Shkumatov, A.V., Tria, G., Kikhney, A.G., Gajda, M., Gorba, C., Mertens, H.D.T., Konarev, P.V. and Svergun, D.I. (2012) New developments in the ATSAS program package for small-angle scattering data analysis. J. Appl. Cryst. 45, 342-350
	Petoukhov, M.V., Konarev, P.V., Kikhney A.G. & Svergun, D.I. (2007) ATSAS 2.1 - towards automated and web-supported small-angle scattering data analysis. J. Appl. Cryst. 40, s223-s228
PRIMUS	Konarev, P.V., Volkov, V.V., Sokolova, A.V., Koch M.H.J. & Svergun, D.I. (2003) PRIMUS: a Windows PC-based system for small-angle scattering data analysis. J. Appl. Cryst. 36, 1277-1282
GNOM	Svergun D.I. (1992) Determination of the regularization parameter in indirect-transform methods using perceptual criteria. J. Appl. Crystallogr. 25, 495-503
DAMMIN	Svergun, D. I. (1999) Restoring low resolution structure of biological macromolecules from solution scattering using simulated annealing. Biophys. J. 76, 2879-2886
DAMMIF	Franke, D. and Svergun, D.I. (2009) DAMMIF, a program for rapid ab-initio shape determination in small-angle scattering. J. Appl. Cryst., 42, 342-346
GASBOR	Svergun, D.I., Petoukhov, M.V. & Koch, M.H.J. (2001) Determination of domain structure of proteins from X-ray solution scattering. Biophys. J. 80, 2946-2953
CRYSOL	Svergun D.I., Barberato C., & Koch M.H.J. (1995) CRYSOL - a program to evalate X-ray solution scattering of biological macromolecules from atomic coordinates. J. Appl. Crystallogr. 28, 768-773
SASREF, BUNCH	Petoukhov, M.V. & Svergun, D.I. (2005) Global rigid body modelling of macromolecular complexes against small-angle scattering data. Biophys. J. 89, 1237-1250
MASSHA	Konarev, P.V., Petoukhov, M.V. & Svergun, D.I. (2001) MASSHA - a graphic system for rigid body modelling of macromolecular complexes against solution scattering data. J. Appl. Crystallogr. 34, 527-532
EOM	Bernado, P., Mylonas, E., Petoukhov, M.V., Blackledge, M., Svergun, D.I. (2007) Structural Characterization of Flexible Proteins Using Small-Angle X-ray Scattering. J. Am. Chem. Soc. 129(17), 5656-5664

Last modified: November 5, 2014