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EMBL Hamburg Biological
Small Angle Scattering
BioSAXS
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EMBO Practical Course on

Solution Scattering from Biological Macromolecules

October 27 - November 3, 2014
EMBL Hamburg, Germany
Organisers: Dmitri Svergun, Al Kikhney, Maxim Petoukhov, Rob Meijers

The course aims at young biochemists/biophysicists and researchers active in related structural methods with little or no experience in solution scattering. The course will cover basics of SAXS/SANS, instrumentation, data collection, modelling techniques and complementary use with other methods. The students are encouraged to bring their own samples to perform synchrotron solution scattering experiments on-site and the results will be used for practical tutorials and discussed on the last day of the course.

BIOSAXS   The course is supported by BIOSAXS.



Abstracts of the talks and slides in PDF

Abstract Scattering of X-rays (basics) (3MB) – Patrice Vachette
Abstract Scattering of neutrons (basics) (14MB) – Cy Jeffries
  Biodeuteration and SANS facilities (0.05MB) – Cy Jeffries
Abstract Sample preparation and characterisation (2MB) – Rob Meijers
Abstract Data reduction and processing (3MB) – Al Kikhney
Abstract Ab initio methods: how/why do they work (3MB) – Dmitri Svergun
Abstract Rigid body refinement (basics) (2MB) – Dmitri Svergun
Abstract Form and structure factors: modeling and interactions (3MB) – Jan Skov Pedersen
Abstract Studies of flexible systems (28MB) – Pau Bernadó
  Analysis of mixtures: tutorial (10MB) – Haydyn Mertens
Abstract Structural investigation of protein and peptide fibrillation (84MB) – Bente Vestergaard
Abstract Joint Use of SAXS and SANS (10MB) – Jill Trewhella
  Joint use of SAXS and NMR (7MB) – Annalisa Pastore
  Membrane proteins: structural and functional challenges (40MB) – Susana Andrade
  Joint use of SAS and AUC (18MB) – Olwyn Byron
Abstract Using SAXS to study DNA regulation (5MB) – Titia Sixma
  High brilliance SAXS on synchrotrons (4MB) – Manfred Roessle
  Microfluidic sample environments (43MB) – Bente Vestergaard
Abstract Validation of biomacromolecular structures (9MB) – Gerard Kleywegt
  SASBDB: a curated repository for small angle scattering data (5MB) – Erica Valentini

Recommended reading

Single copies of the articles below can be downloaded and printed only for the reader's personal research and study.
Books Svergun, D.I., Koch, M.H.J., Timmins, P.A., May, R.P. (2013)
Small Angle X-Ray and Neutron Scattering from Solutions of Biological Macromolecules
Oxford University Press
  Feigin, L.A. & Svergun, D.I. (1987)
Structure Analysis by Small-Angle X-Ray and Neutron Scattering © Plenum Press / Springer
Reviews Blanchet, C.E. & Svergun, D.I. (2013)
Small-angle X-ray scattering on biological macromolecules and nanocomposites in solution. Annu. Rev. Phys. Chem. 64, 37-54 © Annual Reviews DOI
  Mertens H.D.T. & Svergun D.I. (2010)
Structural characterization of proteins and complexes using small-angle X-ray solution scattering. J. Struct. Biol. 172(1), 128-41 © Elsevier DOI
  Svergun, D.I. (2007)
Small-angle scattering studies of macromolecular solutions. J. Appl. Cryst. 40, s10-s17
ATSAS Petoukhov, M.V., Franke, D., Shkumatov, A.V., Tria, G., Kikhney, A.G., Gajda, M., Gorba, C., Mertens, H.D.T., Konarev, P.V. and Svergun, D.I. (2012)
New developments in the ATSAS program package for small-angle scattering data analysis.
J. Appl. Cryst. 45, 342-350
  Petoukhov, M.V., Konarev, P.V., Kikhney A.G. & Svergun, D.I. (2007)
ATSAS 2.1 - towards automated and web-supported small-angle scattering data analysis.
J. Appl. Cryst. 40, s223-s228
PRIMUS Konarev, P.V., Volkov, V.V., Sokolova, A.V., Koch M.H.J. & Svergun, D.I. (2003)
PRIMUS: a Windows PC-based system for small-angle scattering data analysis. J. Appl. Cryst. 36, 1277-1282
GNOM Svergun D.I. (1992)
Determination of the regularization parameter in indirect-transform methods using perceptual criteria. J. Appl. Crystallogr. 25, 495-503
DAMMIN Svergun, D. I. (1999)
Restoring low resolution structure of biological macromolecules from solution scattering using simulated annealing. Biophys. J. 76, 2879-2886
DAMMIF Franke, D. and Svergun, D.I. (2009)
DAMMIF, a program for rapid ab-initio shape determination in small-angle scattering.
J. Appl. Cryst., 42, 342-346
GASBOR Svergun, D.I., Petoukhov, M.V. & Koch, M.H.J. (2001)
Determination of domain structure of proteins from X-ray solution scattering. Biophys. J. 80, 2946-2953
CRYSOL Svergun D.I., Barberato C., & Koch M.H.J. (1995)
CRYSOL - a program to evalate X-ray solution scattering of biological macromolecules from atomic coordinates. J. Appl. Crystallogr. 28, 768-773
SASREF,
BUNCH
Petoukhov, M.V. & Svergun, D.I. (2005)
Global rigid body modelling of macromolecular complexes against small-angle scattering data. Biophys. J. 89, 1237-1250
MASSHA Konarev, P.V., Petoukhov, M.V. & Svergun, D.I. (2001)
MASSHA - a graphic system for rigid body modelling of macromolecular complexes against solution scattering data. J. Appl. Crystallogr. 34, 527-532
EOM Bernado, P., Mylonas, E., Petoukhov, M.V., Blackledge, M., Svergun, D.I. (2007)
Structural Characterization of Flexible Proteins Using Small-Angle X-ray Scattering. J. Am. Chem. Soc. 129(17), 5656-5664

  Last modified: November 5, 2014

© BioSAXS group 2014