Users must verify that samples are both pure and monodisperse as is possible (preferably above 95%) prior to SAXS measurements at P12. Sample purity can be assessed using native gel filtration (size exclusion chromatography, SEC), ultracentrifugation, dynamic light scattering, etc). In particular, sample contaminants with molecular weights higher than a target of interest must be removed from the sample. If the sample is aggregated, the scattering data will be difficult or even impossible to interpret. Please note that a single band on a denaturing/reducing SDS-PAGE gel does not indicate that the sample is pure or monodisperse. It is advised to run, at the very least, both reducing and non-reducing SDS-PAGE as well as native PAGE to assess sample quality.
The chemical composition of the buffer must exactly match that of the sample. The best results are obtained with the last dialysis buffer. Separately prepared buffers may easily lead to mismatch and to severe difficulties during the buffer subtraction procedure.
Sample concentrations for most experiments at P12 span 0.5–10 mg/ml and typically a concentration series is measured for each sample (e.g. 1, 2, 5, 10 mg/ml). The volume of sample required depends on whether samples are measured using the BioSAXS sample changer or SEC-SAXS (see below). For sample changer measurements, it is advised to bring an excess of an exactly-matched buffer (e.g., 10–20 ml) to set up the concentration series for each sample. If the sample is well behaved, high concentration stocks can be diluted before measurement. If the sample is known to undergo aggregation at high concentration, it is best to bring the low concentration stocks, measure them, and then concentrate the samples onsite immediately prior to SAXS.
Sample concentrations must be determined as accurately as possible as the concentrations are used to normalize the scattering data and to obtain critical molecular weight information. At P12, a Nanodrop spectrophotometer is available that spans and absorbance range of 220–750 nm. For most proteins, concentrations can be determined at 280 nm. Bradford assays, unless very-well standardised for a specific protein, are usually not sufficiently precise for determining protein concentration. A refractometer is also available at P12 for estimating concentration that is particularly useful for proteins lacking aromatic amino acids or those with poor Abs 280 nm extinction coefficients.
Potentially toxic samples
We do not collect SAXS data from samples classified as biohazard S2, S3, or S4, e.g. viruses, prions and toxins. Please send us a detailed description of you samples in advance.
Getting samples to P12
If you bring your samples and buffers with you to P12 and require additional laboratory resources, e.g., to make up more buffer, set up sample dialyses, perform SEC (with fraction collection), please book the Sample Preparation and Characterisation (SPC) laboratory at least two weeks prior to arrival.
If you decide to post the samples and matched buffers prior to you arrival, please do so well in advance. Even with courier service (FedEx, DHL, TNT, UPS, etc) allow a week for the samples to arrive. Send frozen samples only on Monday or Tuesday to ensure delivery before Saturday. Note that the samples will be stored exactly according to your instructions. Write these instructions clearly and label the samples. Do not randomly scatter individual loose tubes into dry or blue ice. Collect the individual tubes into a bag or small box, then place the bag/box into the dry or blue ice container. Make sure to seal the tubes (e.g., with parafilm) to prevent 'lid-popping' during shipment.
Address the samples to:
Dr. Dmitri Svergun
EMBL c/o DESY
Notkestr. 85, Geb. 25a
We also offer a post-in SAXS service – either coupled with remote beam line access or, in exceptional circumstances, for the measurements performed by the beamline staff – for users who cannot travel to P12. For the post-in service, samples should be supplied in either specific PCR tube strips or 96 well plates. The catalogue numbers are provided below.
Note: Users travelling to P12 do not need to supply their own tubes or plates, they are provided at the beam line.
Standard SAXS Measurements with the sample changer
Standard 'batch mode' SAXS measurements at P12 are performed using the automated sample changer (Round et al., 2015 Acta Cryst. D 71, 67-75). Using a standard set-up, samples and buffers undergo continuous sample flow during SAXS measurements, generally consisting of 20 × 50 ms exposures, for a total exposure time of 1 s. A complete buffer + sample, wash-and-dry cycle takes approximately 2 min.
Samples and matched buffers are stored under 8–40°C in the sample changer using:
- 8 × 200 μl PCR tube strips;
- 1.5 ml Eppendorf tubes (usually reserved for buffers or water);
- 96 well plates.
PCR strips and 1.5 ml Eppendorf tubes
Lidless 8 × 200 μl PCR tube strips and lidless 1.5 ml Eppendorf tubes are provided for users at the beam line. Usually, samples are pipetted from stock solutions into each PCR tube, while buffers (1 ml) are placed into 1.5 ml Eppendorf tubes prior to loading the sample changer. The sample changer in this configuration has a ~5 μl dead volume, therefore the recommended sample volumes are:
- For standard operation continuous flow measurements: 35 μl (for 30 μl SAXS sample analysis). This setup covers ~80% of user applications.
- For low-volume continuous flow measurements: 25 μl (20 μl analysis).
- For non-standard very low-volume static (i.e., no-flow) measurements: 15–20 μl (10–15 μl analysis).*
*Note 1: Very low sample volume analyses are performed without sample flow enabled during data acquisition. This increases the chances of X-ray induced aggregation to the sample.
Note 2: It is possible to use the PCR strips for the buffers instead of using 1.5 ml tubes. The collection strategy at P12 usually employs two buffer measurements for every sample measurement, e.g., buffer-sample-buffer. Therefore, it is necessary to pipette ~70–100 μl of buffer into the PCR tubes for each sample.
Note 3: For users who opt for our post-in service and wish to pre-prepare their samples in PCR tubes the supplier and catalogue number of the strips and lids are:
Make sure the tubes are labelled and well sealed to prevent 'lid-popping' during transport (especially when using airfreight.)
- Sarstedt. cat# 72.985.992 (Multiply® microstrip PCR Tubes (no lid));
- Sarstedt. cat# 65.989.002 (Multiply® strip of 8 lids).
96 well plates
The BioSAXS sample changer can also use 96 well plates for sample and buffer loading. The dead volume for 96 well plates is larger (~20 μl) compared to the PCR tube option. Therefore it is necessary to load more sample and buffer into each well. For standard measurements, 50 μl of sample (at a minimum) and 100–120 μl of buffer should be loaded per well for a continuous flow SAXS measurement. Again, 96 well plates are provided at the P12 beam line at no cost to users. For those wishing to pre-prepare their 96 well plates prior to arrival, or to use our post-in service, the catalogue numbers for the plates and sealing material are:
- Greiner Bio-One, cat#. 651201 (plates);
- Greiner Bio-One, cat#. 676070 (Platesealer, ViewSeal, Transparent Sealing Film).
We offer a room-temperature triple-detector-array (TDA: UV/RI/RALLS) size exclusion chromatography system in parallel with SAXS that enables:
- the separation of components of polydisperse samples immediately prior to SAXS;
- molecular weight validation of the separated components analysed by UV/RI/RALLS and SAXS.
Note 1. Unless otherwise agreed, SEC-SAXS/TDA experiments are by default collaborative experiments between users and the local P12 contact.
SEC-SAXS requires more sample, 50–100 μl at 7–15 mg/ml and more time (typically 70-80 minutes per sample run). The increased sample and time requirements for SEC-SAXS are due to the pressure limits (i.e., flow rate) and dilution effects of the SEC columns. It is advised to bring, or make up onsite, at least 500 ml of SEC buffer.
- It is recommended that 3% v/v glycerol is added to the SEC column buffer to reduce the effects of radiation damage (see below), assuming that the addition of glycerol does not affect the sample.
- It is recommended that a small sample is measured prior to SEC-SAXS using the default sample changer setup (see above) to assess radiation sensitivity.
Buffers can be made at the SPC facility next to the beam line (requires advanced booking and additional safety training).
Note 2. Users are advised to use their own SEC columns. Dextran-based analytical FPLC columns (e.g., 24 ml Superdex S200 Increase) and, as of 2015, HPLC columns are supported.
Note 3: It may take 1–2 hrs (even more) to appropriately equilibrate a SEC column to a stable RI baseline prior to a SEC-SAXS-TDA measurement. Baseline equilibration is best performed overnight, the day prior to SEC-SAXS measurements.
For more details, please refer to the Malvern box instructions.
Decreasing the effects of radiation damage
Samples can be damaged by X-rays that induce aggregation during the course of exposure which will ruin data interpretation. Radiation damage can be reduced via the addition of:
- 3–5% v/v (maximum) glycerol.
- 1–2 mM dithiothreitol (DTT, note will affect Abs280 nm readings).
- 1–2 mM ascorbic acid (vitamin C).
- 2–5 mM Tris(2-carboxyethyl)phosphine (TCEP; a more stable reducing agent).
- The use of Tris or HEPES buffers.
- The addition of other polyols at low concentration, e.g., mono-ethylene glycol.
Note 1. It is very important to know prior to SAXS that the addition of these small molecules does not destabilise the solute (e.g., cause the protein to aggregate).
Note 2. The addition of polyols, e.g., glycerol, mono-ethylene glycol, sucrose, etc, should not generally exceed 5% v/v as the contrast of the system will be lowered. For SEC-SAXS using dextran FPLC columns, 3% v/v is the recommended maximum due to column pressure limits.
Note 3. The exact same quantities of additive must be added to both the sample and to the matched buffer.
Note 4. It is also possible to attenuate the X-ray beam to reduce the X-ray flux and thus decrease X-ray damage. However, beam attenuation results in a significant reduction in data quality. Please consult with your local contact.
For more details on limiting radiation damage, please refer to Jeffries et al., 2015 J. Synchrotron Rad. 22, 273-279.
Jeffries, C.M., Graewert, M.A., Blanchet, C.E., Langley, D.B., Whitten A.E. and Svergun, D.I. (2016)
Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments
Nature Protocols 11, 2122-2153
Jeffries, C.M., Gräwert, M.A., Svergun, D.I. and Blanchet C.E. (2015)
Limiting radiation damage for high-brilliance biological solution scattering: practical experience at the EMBL P12 beamline
J. Synchrotron Rad. 22, 273-279
© IUCr DOI
Round, A., Felisaz, F., Fodinger, L., Gobbo, A., Huet, J., Villard, C., Blanchet, C.E., Pernot, P., McSweeney, S., Roessle, M., Svergun, D.I. and Cipriani, F. (2015)
BioSAXS Sample Changer: a robotic sample changer for rapid and reliable high-throughput X-ray solution scattering experiments
Acta Cryst. D71, 67-75
© IUCr DOI