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EMBL Hamburg Biological
Small Angle Scattering
BioSAXS
 

SAXS facilities

The resolution of SAXS is up to about 1.5 nm, the method can be used for macromolecules with MW from about 5 kDa up to 100 MDa.

One can study low resolution shape, quaternary structure, complex formation, add missing portions to high resolution models, analyze oligomeric mixtures and kinetic processes, and responses to changes conditions like pH, ionic strength, temperature etc.

For structure analysis (shape, quaternary structure), monodisperse samples are required. Monodispersity must be checked before doing SAXS by DLS, native gel, ultracentrifugation etc.

About 2-3 mg of purified material is needed for a complete study. The typical concentration range starts from about 0.5-1 mg/ml to about 10 mg/ml. At P12 the minimum sample volume per measurement is currently about 20 μl, recommended sample volume is 40 μl. Please check the detailed sample requirements.

If you are interested in a collaborative proposal, EMBL staff will be happy to help you not only with data collection and processing, but also with the interpretation and model building.


  Last modified: June 14, 2018

© BioSAXS group 2018