The resolution of SAXS is up to about 1.5 nm, the method can be used for macromolecules with MW from about 5 kDa up to 100 MDa.
One can study low resolution shape, quaternary structure, complex formation, add missing portions to high resolution models, analyze oligomeric mixtures and kinetic processes, and responses to changes conditions like pH, ionic strength, temperature etc.
For structure analysis (shape, quaternary structure), monodisperse samples are required. Monodispersity must be checked before doing SAXS by DLS, native gel, ultracentrifugation etc.
In SAXS, a concentration series (usually 3–5 concentrations, from 0.5 mg/ml to 10 mg/ml) needs to be measured. At P12, the sample volume for each concentration
is about 40 μl, such that about 2–3 mg of purified material is typically required for a
complete study. Please check the detailed sample requirements.
If you are interested in a collaborative proposal, EMBL staff will be happy to help you not only with data collection and processing, but also with the interpretation and model building.