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EMBO Practical Course on

Solution Scattering from Biological Macromolecules

October 25 - November 1, 2010
European Molecular Biology Laboratory, Hamburg outstation

The course aims at young biochemists/biophysicists and researchers active in related structural methods with little or no experience in solution scattering. The course will cover basics of SAXS/SANS, instrumentation, data collection, modelling techniques and complementary use with other methods.

Organisers: Dmitri Svergun, Manfred Roessle, Maxim Petoukhov, Peter Konarev, Daniel Franke, Al Kikhney.
Tentative programme

Photos

Course photos at Picasa »

SAXS group at Facebook »

Abstracts of the talks and slides in PDF

	Introduction (1MB) - Dmitri Svergun
Abstract	Scattering of X-rays (2MB) - Patrice Vachette
Abstract	Scattering of neutrons (3MB) - Regine Willumeit
Abstract	Data recording, reduction and processing (6MB) - Manfred Roessle
	Data reduction and processing tutorial (3MB) - Peter Konarev
Abstract	Ab initio methods: how/why do they work (4MB) - Dmitri Svergun
	Ab initio modelling tutorial: DAMMIN and DAMMIF (2MB) - Daniel Franke
	Ab initio modelling tutorial: DAMCLUST, GASBOR (1MB) - Maxim Petoukhov
Abstract	Sample preparation and shape determination (1MB) - Katherine Brown
Abstract	Rigid body refinement (basics) (2MB) - Dmitri Svergun
	Rigid body refinement tutorial: MASSHA (5MB) - Peter Konarev
	Rigid body refinement tutorial: SASREF, BUNCH, CORAL (1MB) - Maxim Petoukhov
Abstract	Rigid body modelling: applications to proteins (12MB) - Katherine Brown
Abstract	Form & structure factors (3MB) - Jan Skov Pedersen
	Analysis of mixtures tutorial (25MB) - Peter Konarev
Abstract	Flexible systems (PPT, 35MB) - Pau Bernado
Abstract	WAXS (4MB) - Lee Makowski
Abstract	SAXS and SANS (10MB) - Jill Trewhella
Abstract	Sample preparation and characterization (2MB) - Rob Meijers
Abstract	Fibrillation of proteins (7MB) - Bente Vestergaard
Abstract	Time resolved bioSAXS (15MB) - Naoto Yagi
Abstract	Validation of biomacromolecular structures (7MB) - Gerard Kleywegt

Recommended reading

Single copies of the articles below can be downloaded and printed only for the reader's personal research and study.

Book	Feigin, L.A. & Svergun, D.I. (1987) Structure Analysis by Small-Angle X-Ray and Neutron Scattering © Plenum Press / Springer
Reviews	Mertens H.D.T. & Svergun D.I. (2010) Structural characterization of proteins and complexes using small-angle X-ray solution scattering. J. Struct. Biol. 172(1), 128-41.
	Svergun, D.I. (2007) Small-angle scattering studies of macromolecular solutions. J. Appl. Cryst. 40, s10-s17
	Svergun, D.I. & Koch, M.H.J. (2003) Small-angle scattering studies of biological macromolecules in solution. Rep. Prog. Phys. 66, 1735-1782
ATSAS	Konarev, P.V., Petoukhov, M.V., Volkov, V.V., & Svergun, D.I. (2006) ATSAS 2.1, a program package for small-angle scattering data analysis. J. Appl. Cryst. 39, 277-286.
	Petoukhov, M.V., Konarev, P.V., Kikhney A.G. & Svergun, D.I. (2007) ATSAS 2.1 - towards automated and web-supported small-angle scattering data analysis. J. Appl. Cryst. 40, s223-s228
PRIMUS	Konarev, P.V., Volkov, V.V., Sokolova, A.V., Koch M.H.J. & Svergun, D.I. (2003) PRIMUS: a Windows PC-based system for small-angle scattering data analysis. J. Appl. Cryst. 36, 1277-1282.
GNOM	Svergun D.I. (1992) Determination of the regularization parameter in indirect-transform methods using perceptual criteria. J. Appl. Crystallogr. 25, 495-503
DAMMIN	Svergun, D. I. (1999) Restoring low resolution structure of biological macromolecules from solution scattering using simulated annealing. Biophys. J. 76, 2879-2886.
DAMMIF	Franke, D. and Svergun, D.I. (2009) DAMMIF, a program for rapid ab-initio shape determination in small-angle scattering. J. Appl. Cryst., 42, 342-346.
GASBOR	Svergun, D.I., Petoukhov, M.V. & Koch, M.H.J. (2001) Determination of domain structure of proteins from X-ray solution scattering. Biophys. J. 80, 2946-2953.
CRYSOL	Svergun D.I., Barberato C., & Koch M.H.J. (1995) CRYSOL - a program to evalate X-ray solution scattering of biological macromolecules from atomic coordinates. J. Appl. Crystallogr. 28, 768-773
MASSHA	Konarev, P.V., Petoukhov, M.V. & Svergun, D.I. (2001) MASSHA - a graphic system for rigid body modelling of macromolecular complexes against solution scattering data. J. Appl. Crystallogr. 34, 527-532.
SASREF, BUNCH	Petoukhov, M.V. & Svergun, D.I. (2005) Global rigid body modelling of macromolecular complexes against small-angle scattering data. Biophys. J. 89, 1237-1250
EOM	Bernado, P., Mylonas, E., Petoukhov, M.V., Blackledge, M., Svergun, D.I. (2007) Structural Characterization of Flexible Proteins Using Small-Angle X-ray Scattering. J. Am. Chem. Soc. 129(17), 5656-5664

Last modified: October 25, 2012