EMBO Global Exchange Lecture Course

Structural and Biophysical Methods for Biological Macromolecules in Solution

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• Registration deadline: August 31, 2019
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Slides in PDF

• Introduction - Al Kikhney, Alejandro Panjkovich, Francisco Melo Ledermann
• Scattering of X-rays (basics) - Clément Blanchet
• Neutron scattering (basics) - Jill Trewhella
• SAXS and SANS facilities and experimental practice - Clément Blanchet
• Primary SAXS data reduction and evaluation of the overall geometrical parameters - Al Kikhney
• Ab initio modelling methods - Al Kikhney
• Atomic structure based modeling - Alejandro Panjkovich
• Sample and buffer preparation - Melissa Gräwert
• Limits and potential of in silico target prediction by chemical similarity - Andreas Schüller
• Specificity of Protein-DNA recognition of a long DNA binding motif - Francisco Melo Ledermann
• Introduction to solution NMR - Alexandre Bonvin
• NMR spectral assignment and structural calculations - Lucia Banci
• Recent Advances in Biomolecular NMR - Lucia Banci
• Integrative modeling of biomolecular complexes - Alexandre Bonvin
• Analytical ultracentrifugation - Olwyn Byron
• Joint use of SAXS and SANS - Jill Trewhella
• SAXS studies on mixtures and flexible systems - Melissa Gräwert
• Inline purification methods - Melissa Gräwert
• Processing and analysis of SEC-SAXS data - Alejandro Panjkovich
• Combination of scattering and biophysical methods - Olwyn Byron
• Time-resolved SAXS - Clément Blanchet
• Advanced use of databases in the hybrid structural research: PDB - Kristina Djinović-Carugo
• Hybrid modelling methods using SAXS - Alejandro Panjkovich
• Standards for hybrid and integrative methods - Jill Trewhella