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EMBL Hamburg Biological
Small Angle Scattering
BioSAXS

EMBL Online Course on SAXS, May 2020

EMBL Online Lecture Course on

Solution Scattering from Biological Macromolecules

— June 2, 2020
Tuesdays and Thursdays 9:20-10:30, CEST (UTC +2)
Organisers: Dmitri Svergun, Melissa Gräwert, Al Kikhney

This online course aims at young biochemists/biophysicists and researchers active in related structural methods with little or no experience in solution scattering. The course will cover basics of SAXS, instrumentation, sample preparation, modelling techniques and complementary use with other methods. Participants are encouraged to send their questions/comments through the text chat during/after the lectures.

BIOSAXS   The course is supported by BIOSAXS GmbH.

 

Programme/slides

Recommended reading

Single copies of the articles below can be downloaded and printed only for the reader's personal research and study.
Books Svergun, D.I., Koch, M.H.J., Timmins, P.A., May, R.P. (2013)
Small Angle X-Ray and Neutron Scattering from Solutions of Biological Macromolecules
Oxford University Press
  Feigin, L.A. & Svergun, D.I. (1987)
Structure Analysis by Small-Angle X-Ray and Neutron Scattering © Plenum Press / Springer
Publication gudelines Trewhella et al. (2017)
2017 publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution: an update Acta Crystallogr D Struct Biol 73(9), 710-728 DOI
Reviews Graewert, M.A. and Svergun, D.I. (2020)
A beginner's guide to solution small-angle X-ray scattering (SAXS) Biochem (Lond) 42(1), 36-42 DOI
  Jeffries, C.M., Graewert, M.A., Blanchet, C.E., Langley, D.B., Whitten A.E. and Svergun, D.I. (2016)
Preparing monodisperse macromolecular samples for successful biological small-angle X-ray and neutron-scattering experiments Nature Protocols 11, 2122-2153 DOI
  Blanchet, C.E. & Svergun, D.I. (2013)
Small-angle X-ray scattering on biological macromolecules and nanocomposites in solution Annu. Rev. Phys. Chem. 64, 37-54 © Annual Reviews DOI
  Mertens H.D.T. & Svergun D.I. (2010)
Structural characterization of proteins and complexes using small-angle X-ray solution scattering J. Struct. Biol. 172(1), 128-41 © Elsevier DOI
  Kikhney, A.G. and Svergun, D.I (2015)
A practical guide to small angle X-ray scattering (SAXS) of flexible and intrinsically disordered proteins FEBS Lett. 589(19A), 2570-2577 DOI
ATSAS Franke, D., Petoukhov, M.V., Konarev, P.V., Panjkovich, A., Tuukkanen, A., Mertens, H.D.T., Kikhney, A.G., Hajizadeh, N.R., Franklin, J.M., Jeffries, C.M. and Svergun, D.I. (2017)
ATSAS 2.8: a comprehensive data analysis suite for small-angle scattering from macromolecular solutions.
J. Appl. Cryst. 50, 1212-1225
GNOM Svergun D.I. (1992)
Determination of the regularization parameter in indirect-transform methods using perceptual criteria. J. Appl. Crystallogr. 25, 495-503
DAMMIN Svergun, D. I. (1999)
Restoring low resolution structure of biological macromolecules from solution scattering using simulated annealing. Biophys. J. 76, 2879-2886
DAMMIF Franke, D. and Svergun, D.I. (2009)
DAMMIF, a program for rapid ab-initio shape determination in small-angle scattering.
J. Appl. Cryst., 42, 342-346
GASBOR Svergun, D.I., Petoukhov, M.V. & Koch, M.H.J. (2001)
Determination of domain structure of proteins from X-ray solution scattering. Biophys. J. 80, 2946-2953
CRYSOL Svergun D.I., Barberato C., & Koch M.H.J. (1995)
CRYSOL - a program to evalate X-ray solution scattering of biological macromolecules from atomic coordinates. J. Appl. Crystallogr. 28, 768-773
SASREF,
BUNCH
Petoukhov, M.V. & Svergun, D.I. (2005)
Global rigid body modelling of macromolecular complexes against small-angle scattering data. Biophys. J. 89, 1237-1250
EOM Tria, G., Mertens, H. D. T., Kachala, M. & Svergun, D. I. (2015)
Advanced ensemble modelling of flexible macromolecules using X-ray solution scattering. IUCrJ 2, 207-217
SASBDB Kikhney A.G., Borges C.R., Molodenskiy D.S., Jeffries C.M., Svergun D.I. (2020)
SASBDB: Towards an automatically curated andvalidated repository for biological scattering data Protein Science 29(1); 66-75 DOI

  Last modified: June 3, 2020

© BioSAXS group 2020