EMBL Hamburg Biological
Small Angle Scattering


Automated SAXS data analysis pipeline

Version 3.0 © ATSAS team 2008-2015

High-throughput SAXS experiments became routine with the introduction of sample changing robots, increased accessibility of dedicated high-flux synchrotron radiation beamlines and advanced laboratory X-ray sources. We have developed a set of protocols for automated SAXS data reduction and analysis and implemented them in the SASFLOW data analysis pipeline. The pipeline provides the user with an immediate feedback about the sample characteristics and allows for correcting the sample preparation conditions if necessary. The data processing steps include:

  • radial averaging of the 2D scattering patterns,
  • radiation damage check,
  • background subtraction,
  • evaluation of the overall geometrical parameters:
    • radius of gyration,
    • volume,
    • Dmax,
    • molecular weight,
  • particle flexibility/compactness and
  • ab initio shape reconstruction.

If you use SASFLOW in your work, please cite:
Franke, D., Kikhney, A.G. and Svergun, D.I. (2012) Automated acquisition and analysis of small angle X-ray scattering data. Nuc. Inst. Meth. A. 689, 52-59 © Elsevier DOI

  Last modified: February 17, 2022

© BioSAXS group 2022