Time resolved biological SAXS at SPring-8

Naoto Yagi

Ever since synchrotron radiation became available for X-ray diffraction studies, time-resolved experiments have been attracting much attention. However, the fraction of time-resolved experiments is still low. The technical challenges are two-fold: for the X-ray experiment, a beamline with high intensity and a fast detector are required, and for the biological experiment, a strongly diffracting or scattering sample and a sophisticated trigger method are required. With very high X-ray flux, precaution for radiation damage is mandatory. In this lecture, a few successful (and unsuccessful) experiments made at SPring-8 will be explained.

1. Protein solution scattering
2. Light-induced conformational change of bacteriorhodopsin
3. Skeletal and cardiac muscle diffraction
4. Single-molecule tracking

Date/time: Sunday, 31 October, 9:00