Time resolved biological SAXS at SPring-8
Japan Synchrotron Radiation Research Institute / SPring-8, Japan
Ever since synchrotron radiation became available for X-ray diffraction studies, time-resolved experiments have been attracting much attention. However, the fraction of time-resolved experiments is still low. The technical challenges are two-fold: for the X-ray experiment, a beamline with high intensity and a fast detector are required, and for the biological experiment, a strongly diffracting or scattering sample and a sophisticated trigger method are required. With very high X-ray flux, precaution for radiation damage is mandatory. In this lecture, a few successful (and unsuccessful) experiments made at SPring-8 will be explained.
- Protein solution scattering
- Light-induced conformational change of bacteriorhodopsin
- Skeletal and cardiac muscle diffraction
- Single-molecule tracking
Date/time: Sunday, 31 October, 9:00