DATABSOLUTE may be used to scale concentration normalized data to absolute scale using water as a standard. The forward scattering of water can be calculated as it only depends on the isothermal compressibility and the temperature. To scale a sample to absolute scale a calibration factor needs to be obtained. Data of the water and the empty capillary is collected, and a subtracted curve is obtained. To obtain the forward scattering of water, the intensity plateu between 0.2-0.5A is averaged. Finally, this forward scattering is divided by the calculated forward scattering, resulting in a calibration factor. It is important that the water used for calibration is measured at the same temperature as the sample intended for absolute scaling.
Input files for databsolute may be in any of the supported data formats as long as scattering vector, experimental intensity and errors are available. The scattering of the water should ideally be measured
at the same temperature as the sample intended for absolute scaling. The scattering of the water and the
empty cell should be on the same grid to allow subtraction.