SAXS beamline X33 (shut down since October 2012)
Beamline characteristics
The X33 beamline was located at the D-Fan in the HASYLAB's experimental hall in building 25, DESY. The source of the D-fan was a bending magnet from which the emitted synchrotron radiation was guided to different experimental stations.
The beamline was dedicated to small angle scattering of biological macromolecules, especially to protein and nucleic acids solutions. Scattering experiments on other biological materials such as lipids, on microemulsions and other nanostructured systems were performed as well. Red light setup for light-sensitive samples was available.
Optics
Source
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Bending Magnet
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Monochromator
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Horizontal focusing triangular Si (111) asymmetric cut 7°
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Mirror
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Rhodium coated flat mirror on Zerodur substrate with gravimetrical bending
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Beam size at the detector
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2 x 0.6 mm2
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Wavelength
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0.15 nm
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Flux at sample
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5 x 1011 ph/s/100mA
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Sample to detector distances
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Standard: 2.7 m (other distances possible)
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Resolution SAXS @ 2.7 m
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From s = 0.06 nm-1 to 6 nm-1
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Resolution WAXS*
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From s = 3.8 nm-1 to 17.5 nm-1
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The momentum transfer s = 4π sin(θ)/λ, where 2θ is the scattering angle and λ = 0.15 nm is the X-ray wavelength.

PS - Primary Slits
SS - Secondary Slits
BM - Beam Monitor
Sample stage
Vacuum cell
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30 μl volume, automated filling and rinsing;
temperature range: 7°C to 55°C (up to 70°C for a short time)
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Small automated sample changer
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8 samples (30-200 μl)
3 buffers (1.5 ml)
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Large automated sample changer
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2 x 96 samples (90-200 μl)
2 x 24 buffers (1.5 ml)
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Detectors
2D Photon counting Pilatus 1M-W pixel X-ray detector
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0.172 mm pixel size, 67 x 420 mm2 useful area, 3 ms frame rate.
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2D Photon counting Pilatus 300K-W pixel X-ray detector for WAXS*
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0.172 mm pixel size, 33.5 x 254 mm2 useful area
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Data acquisition: CanOpen fieldbus system.
*The WAXS setup was typically used only on samples with concentration higher than 10 mg/ml.
References
Blanchet, C.E., Zozulya, A.V., Kikhney, A.G., Franke, D., Konarev, P.V., Shang, W., Klaering, R., Robrahn, B., Hermes, C., Cipriani, F., Svergun, D.I and Roessle, M. (2012)
Instrumental setup for high throughput solution scattering at the X33 beamline of EMBL-Hamburg.
J. Appl. Cryst. 45, 489-495
Round, A.R., Franke, D., Moritz, S., Huchler, R., Fritsche, M., Malthan, D., Klaering, R., Svergun, D.I. and Roessle, M. (2008)
Automated sample-changing robot for solution scattering experiments at the EMBL Hamburg SAXS station X33.
J. Appl. Cryst. 41, 913-917
Roessle, M.W., Klaering, R., Ristau, U., Robrahn, B. Jahn, D., Gehrmann, T., Konarev, P. V., Round, A., Fiedler, S., Hermes, S. and Svergun, D. I. (2007)
Upgrade of the Small Angle X-ray scattering Beamline X33 at the European Molecular Biology Laboratory, Hamburg.
J. Appl. Cryst. 40, s190-s194
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